Article
Immunology
Matthew D. Patrick, Ramkumar T. Annamalai
Summary: This study enhances the immunomodulatory potential of mesenchymal stromal cells (MSC) by priming them with IFN gamma and TNF alpha, and develops gelatin-based microgels. These microgels possess adsorption capacity and sustained release effect, showing great potential in immunomodulation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Shaojun Liu, Dongyu Li, Tingting Yu, Jingtan Zhu, Oxana Semyachkina-Glushkovskaya, Dan Zhu
Summary: This study explores the effects of transcranial photobiomodulation on microglial function in diabetic mice and finds that it improves microglial morphology and reactivity, stimulates the brain drainage system, and increases the expression of the microglial purinergic receptor P2RY12. Additionally, transcranial photobiomodulation improves energy expenditure and locomotor activity in diabetic mice.
COMMUNICATIONS BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Raquel Guillamat-Prats
Summary: MSCs play a vital role in controlling various phases in response to injuries, immune reactions, wound healing, and regeneration. They can regulate coagulation pathways, trigger platelet production, and contribute to platelet activation. Proper characterization of MSCs is important to prevent adverse coagulation events.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Brian J. Kwee, Johnny Lam, Adovi Akue, Mark A. KuKuruga, Kunyu Zhang, Luo Gu, Kyung E. Sung
Summary: This study evaluated the immunosuppressive capacity of IFN-gamma-licensed MSCs from multiple donors on different biomaterials, showing that specific integrin expression levels can impact the immunosuppressive capacity of MSCs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Endocrinology & Metabolism
Qiong Wu, Shuangli He, Yu Zhu, Shiming Pu, Zuping Zhou
Summary: This study demonstrated the effectiveness of adipose-derived stromal/stem cells (ASCs) in treating obesity and aging in aged mice. ASCs treatment led to significant weight loss, improved organ function, increased antioxidant capacity, enhanced lipid metabolism, and reduced inflammation in old mice. The positive effects of ASCs suggest their potential as primary antiaging and antiobesity agents for the elderly.
Article
Biochemistry & Molecular Biology
Jin Yoon, Seul Ki Lee, Arum Park, Jiho Lee, Inuk Jung, Kun Baek Song, Eom Ji Choi, Soo Kim, Jinho Yu
Summary: The pathogenesis of atopic dermatitis involves immune dysregulation and epidermal barrier defects. This study investigated the therapeutic effects of IFN-γ-iExo on mice with Aspergillus fumigatus-induced AD. Treatment with IFN-γ-iExo improved clinical and histological outcomes by restoring skin barrier function and suppressing T cell-mediated immune response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Hui Li, Qi Liu, Xiaofeng Gao, Di Zhang, Shengjun Mao, Yongqian Jia
Summary: The study explored the potential of human umbilical mesenchymal stem cells transfected with IFN-γ gene and found that this treatment significantly reduced the incidence and severity of GVHD in vivo, providing a significant survival benefit.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Immunology
Raghavan Chinnadurai, Paul D. Bates, Keith A. Kunugi, Kwangok P. Nickel, Larry A. DeWerd, Christian M. Capitini, Jacques Galipeau, Randall J. Kimple
Summary: MSCs are being tested as a cell therapy in clinical trials for inflammatory disorders, but suitable preclinical animal models are rare. In this study, two robust animal models of immune pathology were introduced to study the effect of IFN gamma primed bone marrow derived MSCs in acute radiation syndrome and graft versus host disease. The results showed that infused IFN gamma primed allogeneic MSCs protect against lethality from ARS, but not GvHD.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Beverly Brooks, Dominique Ebedes, Ahsan Usmani, Joaquin Vega Gonzales-Portillo, Daniel Gonzales-Portillo, Cesario Borlongan
Summary: Ischemic brain injury is a major cause of death worldwide with limited treatment options. Stem cell-based regenerative therapies, specifically mesenchymal stromal cells, show promise in stroke treatment, but further research is needed to fully understand their cell function.
Article
Cell & Tissue Engineering
Ariel Joy Lipat, Chasen Cottle, Bonnie M. Pirlot, James Mitchell, Brian Pando, Brian Helmly, Joanna Kosko, Devi Rajan, Peiman Hematti, Raghavan Chinnadurai
Summary: This study examined the secretory chemokine responses of mesenchymal stromal cells (MSCs) and developed a multiplex analytical method to evaluate their potency. The researchers found that MSCs' innate ability to secrete chemokines is correlated with their metabolic fitness, and the innate secretion of chemokines has a unique correlation pattern. They also found that the combination of IFN gamma and TNF alpha stimulation produces more robust and broad matrix chemokine responses in MSCs compared to individual stimulations. Additionally, the study identified chemokines that can predict immune suppression in MSC and peripheral blood mononuclear cell coculture analysis.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Review
Veterinary Sciences
Benjamin Uberti, Anita Plaza, Claudio Henriquez
Summary: Mesenchymal stem/stromal cells (MSCs) therapy is an important part of regenerative medicine and can modulate immune and inflammatory responses. However, there are discrepancies between the effects of MSCs in clinical applications and the results in experimental models, possibly due to limited survival or impaired function of cells in the inflammatory environment. Pre-conditioning strategies can enhance the survival and efficacy of MSCs.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Cell & Tissue Engineering
Ha Young Park, Chae Eun Kim, Soung-Min Lee, Joo Mi Ahn, Eun Hye Yoon, Minjoo Yoo, Jung-Mi Kim, Jiyeon Back, Dae Hwi Park, Won Hee Jang, Byungsuk Kwon, Su-Kil Seo
Summary: This study found that preconditioning MSCs with IFN-gamma enhances their immunosuppressive activity and the addition of bortezomib attenuates the unfavorable effects of IFN-gamma priming, increasing their immunosuppressive activity. This research provides an important method for MSC-based cell therapy for inflammatory diseases.
Article
Ophthalmology
Jhuwala Venkatakrishnan, Yusef Saeed, Winston W. -Y Kao
Summary: Mesenchymal stem/stromal cells (MSCs) have gained interest due to their differentiation potential, anti-inflammatory effects, and immune-modulating properties. The eye, with its immune-privileged status, is a unique system to study the effects of MSCs. MSCs modulate the immune response by secreting cytokines, growth factors, and extracellular matrix proteins, inhibiting inflammation and promoting healing.
Article
Engineering, Biomedical
Mahsa Haseli, David A. Castilla-Casadiego, Luis Pinzon-Herrera, Alexander Hillsley, Katherine A. Miranda-Munoz, Srikanth Sivaraman, Adrianne M. Rosales, Raj R. Rao, Jorge Almodovar
Summary: The study demonstrates that using multilayers of HEP/COL structure and IFN-gamma can enhance and sustain the immunomodulatory activity of hMSCs, improving their capacity to suppress PBMC proliferation.
MATERIALS TODAY BIO
(2022)
Article
Biochemistry & Molecular Biology
Felipe Bruno Dias de Oliveira, Eliane Antonioli, Olivia Furiama Metropolo Dias, Jean Gabriel de Souza, Sudha Agarwal, Ana Marisa Chudzinski-Tavassi, Mario Ferretti
Summary: This study examined the therapeutic potential of bone marrow MSC transplant through intra-articular and intravenous routes in a preclinical model of osteoarthritis. The results showed that a single injection of MSCs did not effectively inhibit AC degeneration in this model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Huiling Tang, Siru Qin, Wei Li, Xuyi Chen, Luis Ulloa, Qiumei Zhu, Baohu Liu, Yinan Gong, Yadan Zhao, Songtao Wang, Shanshan Li, Yongming Guo, Zhifang Xu, Yi Guo
Summary: Acupuncture at the hand 12 Jing-Well points (HTWP) was found to improve consciousness and neurologic function in TBI rats by activating genes related to neuronal projections, protein secretion, and dopamine receptors in the ventral periaqueductal gray (vPAG). The study also demonstrated that acupuncture alleviates TBI-induced comas via dopamine neurons expressing the P2RX7 receptor in the vPAG, revealing the cellular and molecular mechanisms behind the improvement of TBI clinical outcomes by HTWP acupuncture.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Review
Neurosciences
Zhongxi Lyu, Yongming Guo, Yinan Gong, Wen Fan, Baomin Dou, Ningcen Li, Shenjun Wang, Yuan Xu, Yangyang Liu, Bo Chen, Yi Guo, Zhifang Xu, Xiaowei Lin
Summary: Pain is a global public health priority, with analgesic therapy remaining a challenge. Acupuncture can modulate central sensitization and neuroglial crosstalk and synaptic plasticity to produce analgesic effects.
Article
Integrative & Complementary Medicine
Bao-hu Liu, Dan Zhou, Yi Guo, Sai Zhang, Yong-ming Guo, Tong-tong Guo, Xu-yi Chen, Yi-nan Gong, Hui-ling Tang, Zhi-fang Xu
Summary: Bloodletting puncture at twelve Jing-well points may play a key role in relieving cerebral edema in traumatic brain injury by regulating the expressions of MMP9 and AQP4 as well as inhibiting the ERK and p38 signaling pathways. This therapeutic strategy shows promise for treating TBI-induced cerebral edema.
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
(2021)
Article
Emergency Medicine
Kazuyuki Miyamoto, Shino Katsuki, Hiroki Yamaga, Motoyasu Nakamura, Keisuke Suzuki, Gen Inoue, Masaharu Yagi, Jun Sasaki, Kenji Dohi, Munetaka Hayashi
Summary: Guillain-Barre syndrome (GBS) is a progressive disease characterized by symmetric muscle weakness. Early diagnosis and treatment are crucial for improving patient outcomes. Detailed medical history collection and considering GBS as a differential diagnosis in emergency settings where neurological examination is not feasible are important steps in the diagnostic process.
AMERICAN JOURNAL OF EMERGENCY MEDICINE
(2022)
Article
Integrative & Complementary Medicine
Nannan Yu, Fuming Yang, Xue Zhao, Yongming Guo, Yuan Xu, Guangchang Pang, Yinan Gong, Shenjun Wang, Yangyang Liu, Yuxin Fang, Kun Yu, Lin Yao, Hui Wang, Kuo Zhang, Baohu Liu, Zhenguo Wang, Yi Guo, Zhifang Xu
Summary: This study evaluated the anti-inflammatory potential of manual acupuncture (MA) in treating rheumatoid arthritis (RA) using adjuvant-induced arthritic (AIA) rats. The results showed that MA reduced heat hyperalgesia and paw swelling in AIA rats by inhibiting M1 macrophage polarization and increasing the Treg cell population.
ACUPUNCTURE IN MEDICINE
(2023)
Review
Oncology
Shanshan Li, Suhong Zhao, Yi Guo, Yuanzhen Yang, Jin Huang, Jiaqi Wang, Shanshan Lu, Bin Wang, Chao Chai, Zhifang Xu, Yenlie Chin
Summary: This review summarizes the progress of using acupoint stimulation to combat chemotherapy-associated side effects and assist in inhibiting tumor growth. Acupoint stimulation has been found to alleviate chemotherapy-induced peripheral neuropathy, cognitive impairment, gastrointestinal toxicity, and restore bone marrow hematopoiesis and immune function. It can also promote tumor cell apoptosis, enhance the enrichment of chemotherapeutic agents in tumor tissue, and modulate the tumor immune microenvironment and vasculature. Neuroimmune regulation may be involved in the effects of acupoint stimulation. However, more high-quality clinical studies are needed to confirm its clinical value.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Kazuyuki Miyamoto, Motoyasu Nakamura, Hirokazu Ohtaki, Keisuke Suzuki, Hiroki Yamaga, Kaoru Yanagisawa, Atsuo Maeda, Masaharu Yagi, Munetaka Hayashi, Kazuho Honda, Kenji Dohi
Summary: Global warming increases the incidence of heatstroke, which can cause neurological symptoms and damage to the cerebellum. In a mouse heatstroke model, motor coordination disorder was observed 3 weeks after heatstroke and gradually improved to some extent. Although white matter demyelination was detected in the cerebellum 1 and 3 weeks after heatstroke, it was not found in the corpus callosum. The number of Purkinje cells significantly decreased at 1, 3, and 9 weeks after heatstroke. The intensity of synaptophysin and postsynaptic density-95 temporarily attenuated at 3 weeks but intensified at 9 weeks after heatstroke. Motor coordination loss occurred a few weeks after heatstroke and recovered partially. Late-onset motor impairment may be caused by cerebellar dysfunctions, as assessed by morphological changes in cerebellar white matter and Purkinje cells. The number of Purkinje cells did not recover for 9 weeks, but other factors, including motor coordination, partially recovered, possibly through synaptic reconstruction and cerebellar white matter remyelination. These findings are associated with late-onset neurological deficits and recovery after heatstroke.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Keisuke Suzuki, Kazuyuki Miyamoto, Shutaro Ozawa, Raichi Kawakami, Motoyasu Nakamura, Gen Inoue, Akihito Kato, Yoko Tarumi, Masaharu Yagi, Kenji Dohi
Correction
Multidisciplinary Sciences
Keisuke Suzuki, Kazuyuki Miyamoto, Shutaro Ozawa, Raichi Kawakami, Motoyasu Nakamura, Gen Inoue, Akihito Kato, Yoko Tarumi, Masaharu Yagi, Kenji Dohi
Article
Medicine, General & Internal
Kazuyuki Miyamoto, Yuki Kaki, Shino Katsuki, Atsuo Maeda, Motoyasu Nakamura, Keisuke Suzuki, Masaharu Yagi, Jun Sasaki, Kenji Dohi, Munetaka Hayashi
Summary: This article presents a clinical case of non-ST segment elevation myocardial infarction in an 82-year-old female patient. The patient initially presented with epigastric and back pain, but showed no abnormalities on electrocardiography and serum biomarkers, eventually leading to the diagnosis of subendocardial infarction through CT imaging.
AMERICAN JOURNAL OF CASE REPORTS
(2022)
Article
Emergency Medicine
Shino Katsuki, Kazuyuki Miyamoto, Hiroki Yamaga, Atsuo Maeda, Hiromi Takayasu, Masaharu Yagi, Jun Sasaki, Kenji Dohi, Munetaka Hayashi
Summary: This article reports a case of obstructive shock caused by compression of the right ventricle due to gastric tube dilation. By adjusting the patient's position to promptly release the right ventricular compression, the patient recovered.
AMERICAN JOURNAL OF EMERGENCY MEDICINE
(2022)
Article
Neurosciences
Akira Yoshikawa, Hirokazu Ohtaki, Kazuyuki Miyamoto, SungHyek Kim, Kazunori Hase, Makoto Yoshida, Shotaro Kamijo, Sawa Kamimura, Nobuyoshi Koiwa, Masahiko Izumizaki
Summary: This study used three-dimensional kinematic analysis to evaluate motor dysfunction and recovery in mice with brain damage after mild-intensity running exercise. The results showed that appropriate exercise can recover motor function, and three-dimensional kinematic analysis is a powerful tool for detecting minor behavioral alterations on the affected side and compensatory movements on the unaffected side.
Article
Multidisciplinary Sciences
Kaede Hiruma, Keisuke Suzuki, Akihito Kato, Hiroki Yamaga, Motoyasu Nakamura, Gen Inoue, Yuki Kaki, Kazuyuki Miyamoto, Masaharu Yagi, Kenji Dohi
Summary: A 23-year-old man presented with extensive subcutaneous emphysema after a minor injury, with no active suspicion of necrotizing fasciitis based on tests. He was observed for one day and discharged. The mechanism is unclear.
Article
Biochemistry & Molecular Biology
Keisuke Suzuki, Hiroki Yamaga, Hirokazu Ohtaki, Satoshi Hirako, Kazuyuki Miyamoto, Motoyasu Nakamura, Kaoru Yanagisawa, Takuya Shimada, Tomohiko Hosono, Hitoshi Hashimoto, Kazuho Honda, Kenji Dohi
Summary: Heat stroke is a life-threatening illness caused by high ambient temperatures and humidity. This study found that PACAP knockout mice showed greater resistance to heat exposure compared to wild-type mice, possibly due to lower activity of temperature-sensitive neurons and differences in heat production mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Keisuke Suzuki, Kazuyuki Miyamoto, Takahiro Kanai, Mariko Kurihara, Kazuki Kikuchi, Kohei Harano, Akihito Kato, Masaharu Yagi, Yoshimitsu Ohgiya, Kenji Dohi
Summary: Heat stroke can lead to multiple organ dysfunction and death. Some patients may develop neurological sequelae, such as cerebellar ataxia, during the recovery phase. However, there is currently no reliable method to predict the neurological prognosis, and the usefulness of imaging remains inconclusive.
