Article
Biochemistry & Molecular Biology
Sungtaek Oh, Yura Jang, Chan Hyun Na
Summary: This study used discovery-based approaches and quantitative comparative analyses to identify differential proteins in the cerebrospinal fluid (CSF) of patients with ALS. They found 15 proteins that showed significant differences between ALS and the control group, providing a foundation for biomarker research in ALS.
Article
Biochemistry & Molecular Biology
Hilal A. Rather, Shalini Mishra, Yixin Su, Ashish Kumar, Sangeeta Singh, Biswapriya B. Misra, Jingyun Lee, Cristina M. Furdui, Lindsey R. Hamilton, Robert W. Gould, Susan H. Nader, Michael A. Nader, Gagan Deep
Summary: This study investigates the effects of prenatal cocaine use on postnatal development and long-term neurobiological outcomes. The researchers found that extracellular vesicles (EVs) could potentially serve as biomarkers to assess the adverse effects due to prenatal cocaine exposure.
Article
Genetics & Heredity
Haijie Liu, Ziwen Wang, He Li, Meijie Li, Bo Han, Yuan Qi, Huailu Wang, Juan Gao
Summary: This study identified potential diagnostic biomarkers for multiple sclerosis (MS) - MMP2, C8G, and CFH. Differential proteins in serum and cerebrospinal fluid (CSF) were found to be involved in immune-inflammatory response, neuronal development, cell adhesion, and signaling.
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Yael Hirschberg, Natalia Valle-Tamayo, Oriol Dols-Icardo, Sebastiaan Engelborghs, Bart Buelens, Roosmarijn E. Vandenbroucke, Yannick Vermeiren, Kurt Boonen, Inge Mertens
Summary: Dementia is a leading cause of death worldwide, and this study focused on analyzing the proteome of cerebrospinal fluid (CSF) and CSF-derived extracellular vesicles (EVs) from patients with Alzheimer's disease, dementia with Lewy bodies, and Parkinson's disease dementia. The analysis identified differentially expressed proteins that can be helpful for differential diagnosis and offer new avenues for research into disease-specific pharmacological therapeutics.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Clinical Neurology
Lenka Novakova, Marcus Henricsson, Elias Bjornson, Markus Axelsson, Jan Boren, Igal Rosenstein, Jan Lycke, Susanna L. Cardell, Maria Blomqvist
Summary: The diagnostic utility of cerebrospinal fluid (CSF) sulfatide isoform levels in different courses or phenotypes of multiple sclerosis (MS) was investigated. The results showed that there was no difference in CSF total sulfatide concentrations and isoform distribution among the study groups, and the levels were independent of disease course/phenotype, disease duration, time to conversion to secondary progressive MS, age, and disability.
MULTIPLE SCLEROSIS AND RELATED DISORDERS
(2023)
Article
Biochemistry & Molecular Biology
Tamas Biernacki, Zsofia Kokas, Daniel Sandi, Judit Fuvesi, Zsanett Fricska-Nagy, Peter Farago, Tamas Zsigmond Kincses, Peter Klivenyi, Krisztina Bencsik, Laszlo Vecsei
Summary: MS is a common chronic neurodegenerative disease affecting young people, with significant impact on quality of life. Early recognition and treatment are crucial. Current therapies aim to reduce disease activity, but monitoring measures have limitations. New biomarkers in blood and CSF may improve diagnostic accuracy and personalized treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Arlette L. Bruijstens, Christoph Stingl, Marcel P. Stoop, Yu Yi M. Wong, E. Danielle van Pelt, Brenda L. Banwell, Amit Bar-Or, Theo M. Luider, Rinze F. Neuteboom
Summary: This study analyzed the CSF proteome in pediatric-onset multiple sclerosis (POMS) and identified several proteins associated with POMS, potentially reflecting neurodegeneration, compensatory neuroprotection, and humoral response.
MULTIPLE SCLEROSIS JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Sumaiya Nazli, Kip D. Zimmerman, Angelica M. Riojas, Laura A. Cox, Michael Olivier
Summary: The proteomic analysis of plasma has great potential for precision medicine and biomarker identification. However, detecting and quantifying low abundance proteins in plasma is challenging. This study developed a mass spectrometry approach using tandem mass tags (TMT) and a tissue reference sample, which successfully identified and quantified numerous proteins in nonhuman primate plasma samples. The methodology promises to increase the number of putative disease biomarkers by efficiently quantifying plasma proteins released from specific tissues.
Article
Biochemistry & Molecular Biology
Elizabeth R. Dellar, Iolanda Vendrell, Kevin Talbot, Benedikt M. Kessler, Roman Fischer, Martin R. Turner, Alexander G. Thompson
Summary: This study used mass spectrometry with library-free data-independent acquisition to analyze the CSF proteome of ALS patients. It identified proteins correlated with clinical variables and strengthened the candidacy of CHIT1 and UCHL1 as biomarkers for ALS. The study also highlighted inflammatory and endoplasmic reticulum proteins as potential prognostic biomarkers.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Hans-Wolfgang Klafki, Oliver Wirths, Brit Mollenhauer, Thomas Liepold, Petra Rieper, Hermann Esselmann, Jonathan Vogelgsang, Jens Wiltfang, Olaf Jahn
Summary: Neurochemical biomarkers can aid in the diagnosis of Alzheimer's disease, and the A beta-3-40/A beta 1-42 ratio in blood plasma can accurately predict cerebral amyloid-beta pathology. The presence of A beta-3-40 and A beta-3-38 in human cerebrospinal fluid has been confirmed, and the ratio of CSF A beta-3-40/A beta 42 appears to be increased in individuals with amyloid-beta PET-positive results.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Clinical Neurology
Chihiro Sato, Nipun Mallipeddi, Nupur Ghoshal, Brenton A. Wright, Gregory S. Day, Albert A. Davis, Albert H. Kim, Gregory J. Zipfel, Randall J. Bateman, Audrey Gabelle, Nicolas R. Barthelemy
Summary: High pT217/T217 and low Aβ42/40 were found in CSF of AD patients, while low pT217/T217 and normal Aβ42/40 were observed in 3R+4R tauopathies patients and cognitively normal individuals. The CSF pT217/T217 x CSF Aβ42/40 composite biomarker is sensitive in distinguishing MAPT R406W carriers from other groups.
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Stephanie N. Blandford, Neva J. Fudge, Craig S. Moore
Summary: In this study, elevated levels of CXCL10 were detected in the cerebrospinal fluid (CSF) of multiple sclerosis (MS) cases, and these levels were associated with increased T cell numbers but not with peripheral CXCR3 expression. These findings support the importance of CXCL10 in MS and suggest the presence of alternative mechanisms of CXCL10 beyond solely influencing immune cell trafficking.
Article
Medical Laboratory Technology
Ximeng Chen, Jianan Wang, Jinyang Wang, Jingyun Ye, Ping Di, Chang Dong, Hong Lei, Chengbin Wang
Summary: This study utilized proteomics to analyze serum proteins and identify potential biomarkers for the diagnosis of osteoarticular tuberculosis. ITIH2, CFHR2, CFHR3, and CFHR5 were found to be potential biomarkers, with CFHR2 and CFHR3 showing good diagnostic efficacy when combined in a diagnostic model.
CLINICA CHIMICA ACTA
(2023)
Article
Medicine, Research & Experimental
Fei Ye, Tianzhu Wang, Xiaoxin Wu, Jie Liang, Jiaoxing Li, Wenli Sheng
Summary: This study identified potential diagnostic biomarkers for progressive multiple sclerosis (PMS) based on m6A regulatory genes in the cerebrospinal fluid. The study revealed that the dynamic modification of m6A RNA methylation is involved in the progression of PMS, and could potentially serve as a novel biomarker for diagnosing MS and differentiating PMS from RRMS.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Clinical Neurology
Nik Krajnc, Patrick Altmann, Katharina Riedl, Christoph Mitsch, Thomas Berger, Fritz Leutmezer, Paulus Rommer, Berthold Pemp, Gabriel Bsteh
Summary: This study investigated the association between cerebrospinal fluid (CSF) parameters at the time of multiple sclerosis (MS) diagnosis and retinal thinning. The results showed an inverse association between CSF white blood cell count and retinal thickness at diagnosis. However, other CSF parameters and neurofilament light chain measurements were not associated with retinal thinning.
FRONTIERS IN NEUROLOGY
(2022)
Article
Biochemical Research Methods
Vaibhav Singh, Christoph Stingl, Marcel P. Stoop, Lona Zeneyedpour, Rinze F. Neuteboom, Peter Sillevis Smitt, Rogier Q. Hintzen, Theo M. Luider
JOURNAL OF PROTEOME RESEARCH
(2015)
Article
Neurosciences
Brietta Gerrard, Vaibhav Singh, Olena Babenko, Isabelle Gauthier, V. Wee Yong, Igor Kovalchuk, Artur Luczak, Gerlinde A. S. Metz
Article
Biochemical Research Methods
Marcel P. Stoop, Vaibhav Singh, Christoph Stingl, Roland Martin, Mohsen Khademi, Tomas Olsson, Rogier Q. Hintzen, Theo M. Luider
JOURNAL OF PROTEOME RESEARCH
(2013)
Article
Biochemical Research Methods
Vaibhav Singh, Marcel P. Stoop, Christoph Stingl, Ronald L. Luitwieler, Lennard J. Dekker, Martijn M. van Duijn, Karim L. Kreft, Theo M. Luider, Rogier Q. Hintzen
MOLECULAR & CELLULAR PROTEOMICS
(2013)
Article
Developmental Biology
Archana Bharadwaj Siva, Duvvuri Butchi Kameshwari, Vaibhav Singh, Kadupu Pavani, Curam Sreenivasacharlu Sundaram, Nandini Rangaraj, Mamata Deenadayal, Sisinthy Shivaji
MOLECULAR HUMAN REPRODUCTION
(2010)
Article
Multidisciplinary Sciences
Marcel P. Stoop, Vaibhav Singh, Lennard J. Dekker, Mark K. Titulaer, Christoph Stingl, Peter C. Burgers, Peter A. E. Sillevis Smitt, Rogier Q. Hintzen, Theo M. Luider
Article
Multidisciplinary Sciences
Shiv Verma, Mitali Pandey, Girish C. Shukla, Vaibhav Singh, Sanjay Gupta
Article
Biochemistry & Molecular Biology
Shiv Verma, Eswar Shankar, F. Naz Cemre Kalayci, Amrita Mukunda, Malek Alassfar, Vaibhav Singh, E. Ricky Chan, Gregory T. MacLennan, Sanjay Gupta
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Oncology
Shiv Verma, Eswar Shankar, Spencer Lin, Vaibhav Singh, E. Ricky Chan, Shufen Cao, Pingfu Fu, Gregory T. MacLennan, Lee E. Ponsky, Sanjay Gupta
Summary: This study identified a 3-gene signature panel with prognostic value in bladder cancer, including CDKN2A, CDC20, CTSV, FOXM1, MAGEA6, KRT23, and S100A9. These genes were confirmed to have strong prognostic values in bladder cancer and were validated through qRT-PCR in various human bladder cancer cells representing stage-specific disease progression. Additionally, ULCAN, human protein atlas, and The Cancer Genome Atlas datasets were used to confirm the predictive value of these genes in bladder cancer progression.
Article
Gastroenterology & Hepatology
Vaibhav Singh, Emily Huang, Vai Pathak, Belinda B. Willard, Daniela S. Allende, Laura E. Nagy
Summary: This study investigates the impact of chronic ethanol feeding on the hepatic phosphoproteome, focusing on the death receptor signaling pathways. It is found that chronic ethanol dysregulates these pathways and decreases the inhibitory phosphorylation of ASK1, a critical protein involved in programmed cell death. Furthermore, the study suggests the involvement of noncanonical functions of RIP3 in alcohol-associated liver disease.
HEPATOLOGY COMMUNICATIONS
(2022)
Article
Clinical Neurology
Nagendra Kumar Rai, Vaibhav Singh, Ling Li, Belinda Willard, Ajai Tripathi, Ranjan Dutta
Summary: This study used a proteomic approach to investigate pathobiological mechanisms associated with the progressive phase of multiple sclerosis. The researchers found that mitochondrial proteins exhibited opposite expression patterns in response to demyelination of white- and gray-matter regions in progressive MS brains.
FRONTIERS IN NEUROLOGY
(2021)
Article
Clinical Neurology
Vaibhav Singh, E. Danielle van Pelt, Marcel P. Stoop, Christoph Stingl, Immy A. Ketelslegers, Rinze F. Neuteboom, Coriene E. Catsman-Berrevoets, Theo M. Luider, Rogier Q. Hintzen
NEUROLOGY-NEUROIMMUNOLOGY & NEUROINFLAMMATION
(2015)
