Article
Neurosciences
Takayuki Fuse, Takehiro Nakagaki, Takujiro Homma, Hiroya Tange, Naohiro Yamaguchi, Ryuichiro Atarashi, Daisuke Ishibashi, Noriyuki Nishida
Summary: The study found that in the early stages of prion disease infection, PrPSc is associated with membrane fractions and lipid rafts. Use of DS500 can inhibit the attachment of PrPSc and subsequent prion transmission. This suggests that the early association of PrPSc with the cell surface is crucial for prion infection.
NEUROSCIENCE RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Leonhard H. Urner, Florian Junge, Francesco Fiorentino, Tarick J. El-Baba, Denis Shutin, Gideon Noelte, Rainer Haag, Carol V. Robinson
Summary: Membrane protein purification is crucial for isolating therapeutic targets. However, the role of detergent structure in this process is not well understood, leading to failed preparations and increased costs. This study evaluates the use of hydrophilic-lipophilic balance (HLB) concept to optimize dendritic oligoglycerol detergents. The findings provide qualitative HLB guidelines and enable investigation of lipid binding strengths and protein oligomerization.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Materials Science, Multidisciplinary
Leonhard H. Urner, Ehsan Mohammadifar, Kai Ludwig, Denis Shutin, Francesco Fiorentino, Idlir Liko, Fernando G. Almeida, Daniel Kutifa, Rainer Haag, Carol Robinson
Summary: dPGS-PCL serves as a novel purification agent for membrane protein research, allowing for the study and purification of membrane proteins. Results demonstrate that protein yields and delipidation outcomes are influenced by the properties of membrane mimetics and membranes.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Biology
Benoit Boulan, Charlotte Ravanello, Amandine Peyrel, Christophe Bosc, Christian Delphin, Florence Appaix, Eric Denarier, Alexandra Kraut, Muriel Jacquier-Sarlin, Alyson Fournier, Annie Andrieux, Sylvie Gory-Faure, Jean-Christophe Deloulme
Summary: Neurodevelopmental axonal pathfinding plays a crucial role in brain wiring and cognitive abilities. Sema3E, through its interaction with MAP6 and CRMP4, is shown to be important in promoting axonal growth and development of the fornix. The study highlights the role of CRMP4 in transducing Sema3E signaling and its importance in understanding psychiatric diseases affecting the fornix.
Article
Chemistry, Physical
Alessandra Marega Motta, Maressa Donato, Giovanna Mobbili, Paolo Mariani, Rosangela Itri, Francesco Spinozzi
Summary: This study investigates the mechanisms of interaction between Rhamnolipids (RLs) and simulated plasma membrane models. The results show that mono-RL primarily interacts with the outer leaflet of the membrane through insertion without pore formation, but induces lipid mixing. On the other hand, di-RLs initially interact through insertion and later undergo pore formation.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Review
Chemistry, Physical
Thomas R. Shaw, Subhadip Ghosh, Sarah L. Veatch
Summary: Lateral organization in the plasma membrane plays a crucial role in biological processes. Recent experimental support suggests that lipid organization is important in modulating membrane heterogeneity. The concept of liquid-liquid phase separation explains the observed heterogeneity and predicts responses to membrane composition perturbations.
ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 72
(2021)
Article
Biophysics
Beatrice Barletti, Giacomo Lucchesi, Stefano Muscat, Silvia Errico, Denise Barbut, Andrea Danani, Michael Zasloff, Gianvito Grasso, Fabrizio Chiti, Gabriella Caminati
Summary: Trodusquemine, an amphipathic aminosterol, has shown therapeutic benefit in neurodegenerative diseases by altering the binding of misfolded proteins to the cell membrane. This study investigated the interaction between Trodusquemine and lipid monolayers, and found that Trodusquemine evenly distributed in the lipid monolayer and influenced the organization of lipid rafts within the neuronal membrane in a dose-dependent manner.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Physical
Benedetta Come, Maressa Donato, Lucia Francesca Potenza, Paolo Mariani, Rosangela Itri, Francesco Spinozzi
Summary: Rhamnolipids, produced by Pseudomonas aeruginosa, are glycolipids that act as biosurfactants. Their mechanism of action in biological systems is not well defined, but experimental results suggest that they may alter membrane curvature and structure, potentially affecting endocytic processes in cells.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Multidisciplinary Sciences
Sudhir Sinha, Shashikant Kumar, Komal Singh, Fareha Umam, Vinita Agrawal, Amita Aggarwal, Barbara Imperiali
Summary: Styrene maleic acid lipid particles (SMALPs) were utilized to prepare and characterize membrane proteins of Mycobacterium tuberculosis (Mtb). These nanoparticles contained inner membrane protein PRA, membrane-associated proteins PstS1, LpqH and Ag85, and lipoglycan LAM. Peripheral membrane proteins Acr and PspA were excluded. Additionally, immune responses indicated that only MtM-SMALPs could induce the production of TNF-alpha.
Review
Biochemistry & Molecular Biology
Stanislav Kotlyarov, Anna Kotlyarova
Summary: Atherosclerotic cardiovascular diseases are a significant medical problem with a high prevalence and impact on quality of life and prognosis. Understanding the complex pathogenesis of atherosclerosis and the molecular mechanisms involved can improve diagnostic quality and treatment efficacy.
Article
Biochemistry & Molecular Biology
Rafael Alvarez, Pablo V. Escriba
Summary: GPCR interacts with lipids to bring G proteins together in specific membrane microdomains, enabling efficient signal transduction. The lipid composition and specific protein regions play a crucial role, but the molecular mechanisms underlying the membrane distribution of each G protein isoform are still largely unknown.
Article
Cell Biology
Giulia Lunghi, Emma Veronica Carsana, Nicoletta Loberto, Laura Cioccarelli, Simona Prioni, Laura Mauri, Rosaria Bassi, Stefano Duga, Letizia Straniero, Rosanna Asselta, Giulia Solda, Alessio Di Fonzo, Emanuele Frattini, Manuela Magni, Nara Liessi, Andrea Armirotti, Elena Ferrari, Maura Samarani, Massimo Aureli
Summary: Beta-glucocerebrosidase deficiency leads to cellular accumulation of glucosylceramide and neuronal damage. Impaired neurons respond to lysosomal impairment by increasing biosynthesis and exocytosis. This process also alters plasma membrane architecture and intracellular signaling pathways.
Article
Biochemistry & Molecular Biology
Leonhard H. Urner, Idlir Liko, Kevin Pagel, Rainer Haag, Carol V. Robinson
Summary: Recent studies have highlighted the importance of detergent properties and concentration in membrane protein purification, which may have been overlooked in previous research. The development of rationally designed detergents can enhance the accuracy and reliability of membrane protein studies.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Review
Biochemistry & Molecular Biology
Johanna Detzner, Gottfried Pohlentz, Johannes Muthing
Summary: This review discusses the clinical impact of Enterohemorrhagic Escherichia coli (EHEC) infections, focusing on the vesicular packaging of Shiga toxin (Stx) in the intestine and blood stream, as well as the extraintestinal complications and therapeutic options. The role of epithelial cells of the colon and kidneys in the infection process is also addressed. The review highlights the importance of Stx-binding glycosphingolipids (Gb3Cer and Gb4Cer) and their distribution in primary human kidney and colon epithelial cells. The susceptibility of primary renal and colonic epithelial cells to Stx is discussed, suggesting a resilience of the intestinal epithelium against the human-pathogenic Stx subtypes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Cerina Chhuon, Luis Vicente Herrera-Marcos, Shao-Yu Zhang, Cecile Charriere-Bertrand, Vincent Jung, Joanna Lipecka, Berkan Savas, Nour Nasser, Andre Pawlak, Hocine Boulmerka, Vincent Audard, Dil Sahali, Ida Chiara Guerrera, Mario Ollero
Summary: This study compared different methods of plasma preparation, analyzed the plasma proteomes of patients with recurrent focal and segmental glomerulosclerosis (FSGS) and controls, and investigated the effects of patient plasma on podocytes. The results showed that proteins involved in neutrophil degranulation were significantly increased in the plasma of FSGS patients, while lipid-binding proteins associated with lipoproteins were decreased. Additionally, amino acid transporters involved in mTORC1 activation were found to be significantly increased in extracellular vesicles (EV) from FSGS patients. Furthermore, the study revealed profound differences in proteins involved in the mTOR pathway, autophagy, and cytoskeleton organization in podocytes exposed to patient plasma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Pei-Yang Gao, Ya-Nan Ou, Yi-Ming Huang, Zhi-Bo Wang, Yan Fu, Ya-Hui Ma, Qiong-Yao Li, Li-Yun Ma, Rui-Ping Cui, Yin-Chu Mi, Lan Tan, Jin-Tai Yu
Summary: Liver function may play a role in the progression of Alzheimer's disease. The study found that as AD progressed, certain liver function markers increased while others decreased. The relationship between liver function and CSF AD biomarkers indicates a potential mediation effect on cognition.
JOURNAL OF NEUROCHEMISTRY
(2024)