Review
Biotechnology & Applied Microbiology
Liang Zhang, Xinlin Liang, Tomas Takac, George Komis, Xiaojuan Li, Yuan Zhang, Miroslav Ovecka, Yanmei Chen, Jozef Samaj
Summary: In plants, membrane compartmentalization relies on vesicle trafficking for organelle communication. Membrane proteins involved in vesicle trafficking are dynamic and responsive to environmental changes and cellular signals. Therefore, understanding the localization and dynamics of these proteins is crucial for dissecting vesicular trafficking pathways.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Multidisciplinary Sciences
Fan Fan, Yumei Wu, Manami Hara, Adam Rizk, Chen Ji, Dan Nerad, Natalia Tamarina, Xuelin Lou
Summary: This study revealed the crucial role of dynamins in endocytosis and secretion capacity of pancreatic beta cells, indicating that deficiency in dynamin-mediated endocytosis can lead to impaired insulin secretion, increasing the risk of endocrine dysfunction and diabetes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Kaitlyn E. Fouke, M. Elizabeth Wegman, Sarah A. Weber, Emily B. Brady, Cristina Roman-Vendrell, Jennifer R. Morgan
Summary: Synuclein plays a key role in regulating the clustering of synaptic vesicles at synapses, with perturbations causing a dose-dependent reduction in the number of vesicles. This protein also affects the docking of vesicles at the active zone, influencing the size and distribution of vesicle clusters. These findings provide insights into the role of synuclein in neurotransmission and its implications for diseases like Parkinson's.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Plant Sciences
Xiaobo Zhu, Junjie Yin, Hongming Guo, Yuping Wang, Bingtian Ma
Summary: The vesicle trafficking apparatus is crucial for maintaining the homeostasis of membrane-enclosed organelles in eukaryotic cells. Research on vesicle trafficking in plants, particularly rice, is limited, and further investigations are needed to uncover the mysteries of this system.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Janin Lautenschlager
Summary: This article introduces the emerging concept of protein liquid-liquid phase separation (LLPS) at the synapse, discusses the LLPS tendencies of presynaptic proteins, and highlights key proteins and recent progress in the field.
Article
Cell Biology
Yi-Jheng Peng, Junhua Geng, Ying Wu, Cristian Pinales, Jennifer Langen, Yen-Ching Chang, Christopher Buser, Karen T. Chang
Summary: The study identifies Mnb and Synj as novel regulators of ADBE, and reveals that CME and ADBE are differentially governed by the dual phosphatase domains of Synj.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Michael A. Cousin
Summary: Synaptobrevin-2 (Syb2) is essential for neurotransmitter release, while Synaptophysin (Syp) has been debated for its role. Recent studies indicate that Syp plays a role in ensuring efficient retrieval of Syb2 during SV endocytosis.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Jiapei Yang, Li Xu, Ling Di, Yue Su, Xinyuan Zhu
Summary: PEG, as the gold standard for stealth polymer materials, is widely used in drug delivery due to its excellent properties. However, the lack of understanding about the fate of PEG at the cellular level has hindered its application in diagnosis and therapy. Research showed that PEG could be internalized into cells in 1 hour, localized to lysosomes, cytosol, ER, and mitochondria. The fate of PEG in cells could be regulated by conjugating different small molecules, which is crucial for the rational design of PEGylation for drug delivery.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Biochemistry & Molecular Biology
Katherine Bonnycastle, Elizabeth C. Davenport, Michael A. Cousin
Summary: The fusion, retrieval, and recycling of synaptic vesicles is crucial for neurotransmission maintenance. Mutations in presynaptic genes are increasingly linked to neurodevelopmental disorders such as autism, intellectual disability, and epilepsy. Understanding how mutations in different stages of the synaptic vesicle life cycle can lead to disease may guide future therapies targeting presynaptic function.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Neurosciences
Wonchul Shin, Lisi Wei, Gianvito Arpino, Lihao Ge, Xiaoli Guo, Chung Yu Chan, Edaeni Hamid, Oleg Shupliakov, Christopher K. E. Bleck, Ling-Gang Wu
Summary: This study reveals that endocytosis is mainly mediated by calcium-triggered and dynamin-mediated closure of Omega profiles and fusion pores, leading to the formation of Lambda-shaped, Omega-shaped, and O-shaped vesicles, rather than the traditional flat-to-round membrane transformation.
Review
Cell Biology
Fabian Link, Alyssa R. Borges, Nicola G. Jones, Markus Engstler
Summary: Trypanosoma brucei is a unicellular pathogen that thrives extracellularly in vertebrate hosts, with its cell surface playing a critical role in both immune recognition and evasion. The variant surface glycoprotein (VSG) and incessant motility contribute to immune evasion, while endo- and exocytosis occur at the flagellar pocket. Mechanisms behind VSG recycling and secretion are not fully understood, presenting longstanding questions that may be answered with novel technologies in the future.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Jacopo Meldolesi
Summary: In addition to conventional protein secretion, there are unconventional secretion mechanisms mediated by extracellular vesicles (exosomes and ectosomes). These vesicles are formed within cells and released into the extracellular fluid, creating continuous networks for exchanging molecules and information. The proteins secreted via these unconventional mechanisms play important roles in cellular physiology and diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lucas Bayones, Samuel Alfonso-Bueno, Mauricio Montenegro, Jose Moya-Diaz, Octavio Caspe, Luciana Gallo, Fernando D. Marengo
Summary: This study investigated the endocytosis and replenishment of the immediately releasable pool (IRP) in mice primary chromaffin cells. The researchers found that not only a fast dynamin-dependent endocytosis occurred after the release of about 50% IRP, but also a fast dynamin-independent endocytosis was activated after depolarizations lasting 25-50ms, which released over 80% of IRP. This dynamin-independent endocytosis was found to be Ca2+- and protein kinase C (PKC)-dependent. Additionally, PKC inhibition also affected the slow component of replenishment observed after total IRP exocytosis.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Neurosciences
Haiyan Li, Maia Datunashvili, Reno C. C. Reyes, Susan M. M. Voglmaier
Summary: Inositol pyrophosphates play a crucial role in cellular signaling and membrane trafficking, particularly in synaptic vesicle recycling. Two isoforms of Inositol hexakisphosphate kinases (IP6Ks), IP6K1 and IP6K3, are expressed in axons and regulate vesicle recycling through different endocytic pathways. IP6Ks also affect synaptic facilitation and glutamate signaling.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Marat A. Mukhamedyarov, Aydar N. Khabibrakhmanov, Venera F. Khuzakhmetova, Arthur R. Giniatullin, Guzalia F. Zakirjanova, Nikita V. Zhilyakov, Kamilla A. Mukhutdinova, Dmitry V. Samigullin, Pavel N. Grigoryev, Andrey V. Zakharov, Andrey L. Zefirov, Alexey M. Petrov
Summary: Amyotrophic lateral sclerosis (ALS) is a disease characterized by skeletal muscle denervation, motor neuron loss, and respiratory failure. Mutations in the FUS gene are a common genetic cause of ALS, resulting in degeneration. In mutant FUS mice at the pre-onset stage, early structural and functional alterations in diaphragm neuromuscular junctions (NMJs) were observed. Lipid peroxidation and decreased lipid raft staining were found in the mutant mice. Despite preserved end-plate structure, increased levels of presynaptic proteins and impaired neurotransmitter release were observed in FUS mice. These findings suggest that alterations in membrane properties, synapsin 1 levels, and calcium kinetics could be early indicators of nascent NMJ pathology in ALS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Erez Eitan, Carmel Braverman, Ailone Tichon, Daniel Gitler, Emmette R. Hutchison, Mark P. Mattson, Esther Priel
Article
Emergency Medicine
Itai Shavit, Eliaz Brumer, Danielle Shavit, Mark Eidelman, Ivan P. Steiner, Carmit Steinberg
ANNALS OF EMERGENCY MEDICINE
(2016)
Article
Cell Biology
Archan Ganguly, Xuemei Han, Utpal Das, Lina Wang, Jonathan Loi, Jichao Sun, Daniel Gitler, Ghislaine Caillol, Christophe Leterrier, John R. Yates, Subhojit Roy
JOURNAL OF CELL BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Eden Shusterman, Ofer Beharier, Shiri Levy, Raz Zarivach, Yoram Etzion, Craig R. Campbell, Il-Ha Lee, Anuwat Dinudom, David I. Cook, Asher Peretz, Amos Katz, Daniel Gitler, Arie Moran
Article
Multidisciplinary Sciences
Robert Mertens, Sarah Melchert, Daniel Gitler, Morten Brix Schou, Sverre Georg Saether, Arne Vaaler, Johannes Piepgras, Elena Kochova, Fabio Benfenati, Gudrun Ahnert-Hilger, Klemens Ruprecht, Markus Hoeltje
Article
Multidisciplinary Sciences
Merav Atias, Yaara Tevet, Jichao Sun, Alexandra Stavsky, Shani Tal, Joy Kahn, Subhojit Roy, Daniel Gitler
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Meir Schechter, Merav Atias, Suaad Abd Elhadi, Dana Davidi, Daniel Gitler, Ronit Sharon
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Letter
Clinical Neurology
Zagorka Vitic, Hazem Safory, Vukasin M. Jovanovic, Yael Sarusi, Alexandra Stavsky, Joy Kahn, Alona Kuzmina, Lilah Toker, Daniel Gitler, Ran Taube, Roland H. Friedel, Simone Engelender, Claude Brodski
Article
Endocrinology & Metabolism
Mohamad-Reza Aghanoori, Vicky Margulets, Darrell R. Smith, Lorrie A. Kirshenbaum, Daniel Gitler, Paul Fernyhough
Summary: The study demonstrated decreased glycolytic capacity and impaired mitochondrial respiration in DRG neurons from type 1 diabetic rats. IGF-1 was found to protect against ATP depletion in DRG neurons from diabetic rats by enhancing glycolysis and mitochondrial respiration parameters.
MOLECULAR METABOLISM
(2021)
Article
Biology
Alexandra Stavsky, Ohad Stoler, Marko Kostic, Tomer Katoshevsky, Essam A. Assali, Ivana Savic, Yael Amitai, Holger Prokisch, Steffen Leiz, Cornelia Daumer-Haas, Ilya Fleidervish, Fabiana Perocchi, Daniel Gitler, Israel Sekler
Summary: NCLX plays a crucial role in regulating presynaptic calcium transients that define synaptic strength, short- and long-term plasticity, which are essential elements of learning and memory processes.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
D. Halperin, A. Stavsky, R. Kadir, M. Drabkin, O. Wormser, Y. Yogev, V Dolgin, R. Proskorovski-Ohayon, Y. Perez, H. Nudelman, O. Stoler, B. Rotblat, T. Lifschytz, A. Lotan, G. Meiri, D. Gitler, O. S. Birk
Summary: The authors identified a mutation in CDH2 associated with ADHD and used a mouse model to delineate molecular electrophysiological characteristics associated with this mutation. This study sheds light on the role of CDH2-related pathways in the pathophysiology of ADHD.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Ohad Stoler, Alexandra Stavsky, Yana Khrapunsky, Israel Melamed, Grace Stutzmann, Daniel Gitler, Israel Sekler, Ilya Fleidervish
Summary: Mitochondrial activity plays a crucial role in synaptic plasticity, and the firing pattern of pre- and postsynaptic neurons affects mitochondrial function. Our study revealed that postsynaptic spike firing induces rapid mitochondrial Ca2+ responses, with larger responses observed in cell bodies and apical dendrites. Additionally, the coincidence of an EPSP with a backpropagating spike generated prominent mitochondrial Ca2+ hotspots. These findings suggest that mitochondria decode spike firing patterns into Ca2+ signals, which in turn influence metabolic output and may lead to long-term changes in synaptic efficacy.
Article
Clinical Neurology
Shamchal Bakavayev, Alexandra Stavsky, Shirel Argueti-Ostrovsky, Galit Yehezkel, Yael Fridmann-Sirkis, Zeev Barak, Daniel Gitler, Adrian Israelson, Stanislav Engel
Summary: The researchers developed an antibody scFv-SE21 that can target the structural element responsible for the neurotoxicity of misfolded SOD1, and showed that it delays disease onset and extends survival in ALS mice.
Article
Immunology
Markus Hoeltje, Robert Mertens, Morten Brix Schou, Sverre Georg Saether, Elena Kochova, Sven Jarius, Harald Pruess, Lars Komorowski, Christian Probst, Friedemann Paul, Judith Bellmann-Strobl, Daniel Gitler, Fabio Benfenati, Johannes Piepgras, Gudrun Ahnert-Hilger, Klemens Ruprecht
BRAIN BEHAVIOR AND IMMUNITY
(2017)
Letter
Clinical Neurology
Zagorka Vitic, Hazem Safory, Vukasin M. Jovanovic, Yael Sarusi, Alexandra Stavsky, Joy Kahn, Alona Kuzmina, Lilah Toker, Daniel Gitler, Ran Taube, Roland H. Friedel, Simone Engelender, Claude Brodski
Article
Biochemistry & Molecular Biology
Pei-Yang Gao, Ya-Nan Ou, Yi-Ming Huang, Zhi-Bo Wang, Yan Fu, Ya-Hui Ma, Qiong-Yao Li, Li-Yun Ma, Rui-Ping Cui, Yin-Chu Mi, Lan Tan, Jin-Tai Yu
Summary: Liver function may play a role in the progression of Alzheimer's disease. The study found that as AD progressed, certain liver function markers increased while others decreased. The relationship between liver function and CSF AD biomarkers indicates a potential mediation effect on cognition.
JOURNAL OF NEUROCHEMISTRY
(2024)