Journal
JOURNAL OF NEUROCHEMISTRY
Volume 111, Issue 6, Pages 1447-1456Publisher
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1471-4159.2009.06413.x
Keywords
hypoxia-ischemia; inflammation; microglia; neurogenesis; radiotherapy; stroke
Categories
Funding
- Swedish Research Council
- Swedish Childhood Cancer Foundation (Barncancerfonden)
- Swedish governmental
- Torsten and Ragnar Soderbergs stiftelse
- National Natural Science Foundation of China [CZ:30470598]
- King Gustav V Jubilee Clinic Research Foundation
- Wilhelm and Martina Lundgren Foundation
- Frimurare Barnhus Foundation
- Gothenburg Medical Society
- Swedish Society of Medicine
- Swedish Research Links Program through the Swedish International Development Cooperation Agency (SIDA)
Ask authors/readers for more resources
Cranial radiotherapy is common in pediatric oncology. Our purpose was to investigate if irradiation (IR) to the immature brain would increase the susceptibility to hypoxic-ischemic injury in adulthood. The left hemisphere of postnatal day 10 (P10) mice was irradiated with 8 Gy and subjected to hypoxia-ischemia (HI) on P60. Brain injury, neurogenesis and inflammation were evaluated 30 days after HI. IR alone caused significant hemispheric tissue loss, or lack of growth (2.8 +/- 0.42 mm3, p < 0.001). Tissue loss after HI (18.2 +/- 5.8 mm3, p < 0.05) was synergistically increased if preceded by IR (32.0 +/- 3.5 mm3, p < 0.05). Infarct volume (5.1 +/- 1.6 mm3) nearly doubled if HI was preceded by IR (9.8 +/- 1.2 mm3, p < 0.05). Pathological scoring revealed that IR aggravated hippocampal, cortical and striatal, but not thalamic, injury. Hippocampal neurogenesis decreased > 50% after IR but was unchanged by HI alone. The number of newly formed microglia was three times higher after IR + HI than after HI alone. In summary, IR to the immature brain produced long-lasting changes, including decreased hippocampal neurogenesis, subsequently rendering the adult brain more susceptible to HI, resulting in larger infarcts, increased hemispheric tissue loss and more inflammation than in non-irradiated brains.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available