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Biochemistry & Molecular Biology
Yaobin Liu, Rukmini Mukherjee, Florian Bonn, Thomas Colby, Ivan Matic, Marius Glogger, Mike Heilemann, Ivan Dikic
Summary: The SidE family of Legionella effectors, particularly SdeA, catalyzes non-canonical phosphoribosyl-linked ubiquitination of host proteins to disrupt Golgi integrity. This disruption prevents Golgi linking proteins GRASP55 and GRASP65 from clustering and forming oligomeric structures on multiple serine residues, impacting the host secretory pathway. The Golgi manipulation strategy by Legionella highlights how the bacterium hijacks the secretory pathway to promote bacterial infection.
CELL DEATH AND DIFFERENTIATION
(2021)
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Biochemistry & Molecular Biology
Richard Van Krieken, Yuan-Li Tsai, Anthony J. Carlos, Dat P. Ha, Amy S. Lee
Summary: Recent research has revealed that ER chaperones such as GRP78 can escape from the ER and traffic to the cell surface in colon and lung cancer via a non-traditional endosomal transport pathway mediated by Rab GTPases. This unconventional translocation mechanism is driven by membrane fusion between ER-derived vesicles and endosomes, requiring specific SNARE proteins and co-chaperones regulated by ER stress-induced signaling pathways.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
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Neurosciences
Prachi Ojha, Subhajit Pal, Samarjit Bhattacharyya
Summary: Norbin protein plays a critical role in the internalization process of Group I mGluRs and may be associated with mGluR-mediated synaptic plasticity.
JOURNAL OF NEUROSCIENCE
(2022)
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Biochemistry & Molecular Biology
Lowiese Desmarets, Adeline Danneels, Julien Burlaud-Gaillard, Emmanuelle Blanchard, Jean Dubuisson, Sandrine Belouzard
Summary: The membrane protein of Middle East respiratory syndrome coronavirus (MERS-CoV) plays a crucial role in the virus assembly process. Proper intracellular trafficking and subcellular localization of the M protein are essential for virus formation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
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Biochemistry & Molecular Biology
Leena Haataja, Anoop Arunagiri, Anis Hassan, Kaitlin Regan, Billy Tsai, Balamurugan Dhayalan, Michael A. Weiss, Ming Liu, Peter Arvan
Summary: Efficient export of proinsulin from the endoplasmic reticulum requires the formation of specific disulfide bonds, while disrupting certain pairings may improve trafficking in some mutants. Mutations causing Mutant INS gene-induced Diabetes of Youth (MIDY) often result in perturbed proinsulin disulfide bond formation, with potential for allele-specific pharmacological targeting in the future.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Dan Wu, Lushan Xu, Wen-Min Cai, Shi-Yu Zhan, Guoqiang Wan, Yun Xu, Yun Stone Shi
Summary: TMEM63B is a mechanosensitive cation channel that is activated by hypoosmotic stress and mechanic stimulation. The brain-specific alternative splicing of exon 4 in TMEM63B results in a short isoform that exhibits enhanced responses to hypoosmotic stimulation compared to the long isoform. We found that the difference in response is due to the stronger cell surface expression of the short isoform and the binding of the long isoform to COPI retrieval vesicles, leading to its retrotranslocation to the endoplasmic reticulum (ER). Furthermore, the long isoforms can form heterodimers with short isoforms and reduce their surface expression.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
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Neurosciences
Yong Liu, You Wang, Juan Yang, Tao Xu, Changhong Tan, Peng Zhang, Qiankun Liu, Yangmei Chen
Summary: The study identified that GIPC1 is downregulated in patients with epilepsy and mice with induced epilepsy, and it interacts with mGluR7 to regulate epileptogenesis by increasing mGluR7 expression.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
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Neurosciences
Anna Bodzeta, Nicky Scheefhals, Harold D. MacGillavry
Summary: The various functions of glutamate in the brain are mediated by ionotropic and metabotropic glutamate receptors, which modulate synaptic transmission and plasticity. mGluRs play roles at both presynaptic and postsynaptic sites, acting as essential signal integrators that couple mechanisms of transmission and plasticity.
Editorial Material
Cell Biology
Jeffrey Knupp, Yu-Jie Chen, Anoop Arunagiri, Leena Haataja, Peter Arvan, Billy Tsai
Summary: The study reveals that RTN3 recruits misfolded prohormones for lysosomal degradation through PGRMC1, selectively targeting small oligomers rather than large protein aggregates. This finding suggests that PGRMC1 could be a potential intervention point for diseases caused by ER protein retention.
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Biochemistry & Molecular Biology
Yonis Bare, Tamas Matusek, Sophie Vriz, Maika S. Deffieu, Pascal P. Therond, Raphael Gaudin
Summary: This study demonstrates that newly-synthesised Sonic Hedgehog (SHH) is trafficked through the classical biosynthetic secretory pathway using TMED10 as a cargo receptor, and Rab6 vesicles for Golgi-to-cell surface trafficking. Depletion of TMED10 delays SHH loading onto ER exit sites (ERES) and impairs SHH release. Additionally, the homologue of TMED10, Baiser, is found to participate in Hedgehog (Hh) secretion and signalling in Drosophila wing imaginal disc model. Therefore, TMED10 plays a crucial role in SHH secretion and its interaction with other proteins affects embryonic development.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Microbiology
Anthony V. Morante, Daniel Davidnan Baboolal, Xavier Simon, Elizabeth, Mark Cheng-Ying Pan, Patricio I. Meneses
Summary: The amino acid residues 43-DQILQ-47 of the HPV16 L2 protein play a crucial role in the intracellular trafficking of the virus, particularly in trafficking beyond the endoplasmic reticulum.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Na Wang, Dan-Dan Wang, Xiaolin Hou, Xiumao Li, Ying Shen
Summary: Numb protein isoforms Numb-p72 and Numb-p65 exhibit distinct expression patterns in HEK293T cells, with Numb-p72 specifically binding to mGluR5 alpha and enhancing its membrane expression by inhibiting internalization. This indicates that a complete structure of Numb is necessary for binding to and modulating mGluR5 trafficking.
MOLECULAR BIOLOGY REPORTS
(2021)
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Biochemistry & Molecular Biology
Kosuke Yokobori, Masahiko Negishi
Summary: Phosphorylation of androgen receptor at Ser815 plays a crucial role in regulating its functions and biological roles, including stabilizing homodimer formation and influencing ER stress responses.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Timothy J. Hawkins, Michaela Kopischke, Patrick J. Duckney, Katarzyna Rybak, David A. Mentlak, Johan T. M. Kroon, Mai Thu Bui, A. Christine Richardson, Mary Casey, Agnieszka Alexander, Geert De Jaeger, Monika Kalde, Ian Moore, Yasin Dagdas, Patrick J. Hussey, Silke Robatzek
Summary: This study reveals that NET4A and NET4B members of the NET family play an essential role in the reorganization of guard cell actin during stomatal closure, mediating the molecular link between actin filaments and the tonoplast through interaction with RABG3b.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Vineta Fellman, Rishi Banerjee, Kai-Lan Lin, Ilari Pulli, Helen Cooper, Henna Tyynismaa, Jukka Kallijarvi
Summary: Whole genome sequencing identified a homozygous SERAC1 mutation in a newborn with MEGDHEL syndrome, leading to severe mitochondrial structural and functional abnormalities, which resulted in multiorgan failure.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Pei-Yang Gao, Ya-Nan Ou, Yi-Ming Huang, Zhi-Bo Wang, Yan Fu, Ya-Hui Ma, Qiong-Yao Li, Li-Yun Ma, Rui-Ping Cui, Yin-Chu Mi, Lan Tan, Jin-Tai Yu
Summary: Liver function may play a role in the progression of Alzheimer's disease. The study found that as AD progressed, certain liver function markers increased while others decreased. The relationship between liver function and CSF AD biomarkers indicates a potential mediation effect on cognition.
JOURNAL OF NEUROCHEMISTRY
(2024)