Article
Chemistry, Multidisciplinary
Richiro Ushimaru, Lide Cha, Shotaro Shimo, Xiaojun Li, Jared C. Paris, Takahiro Mori, Kazunori Miyamoto, Lindsay Coffer, Masanobu Uchiyama, Yisong Guo, Wei-chen Chang, Ikuro Abe
Summary: In this study, the catalytic mechanism and stereodivergency of BelL and HrmJ enzymes were investigated. The results showed that the nitroalkane moiety of the substrate is deprotonated to produce the nitronate form, and an iron(IV)-oxo species abstracts proS-H from C4 to initiate intramolecular C-C bond formation. Additionally, the study discovered new homologues that generate cis-configured Ncpa products with different stereochemistries, and identified the critical amino acid residues controlling the stereoselectivity of cyclopropanation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Lide Cha, Jared C. Paris, Brady Zanella, Martha Spletzer, Angela Yao, Yisong Guo, Wei-chen Chang
Summary: In this study, in silico methods were used to identify enzymes that have the potential to introduce aziridine rings. Enzymatic activity was reconstituted in vitro to validate the candidates, and it was found that an iron(IV)-oxo species initiates the formation of aziridine rings through C-H bond cleavage. Additionally, the reaction pathway was diverted from aziridination to hydroxylation using mechanistic probes. Isotope tracing experiments and quantitative product analysis provided evidence for the polar capture of a carbocation species by the amine in the pathway to aziridine installation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Rohan Jonnalagadda, Antonio Del Rio Flores, Wenlong Cai, Rimsha Mehmood, Maanasa Narayanamoorthy, Chaoxiang Ren, Jan Paulo T. Zaragoza, Heather J. Kulik, Wenjun Zhang, Catherine L. Drennan
Summary: The isonitrile moiety is essential in marine sponges and some microbes for various processes. Two biosynthetic routes have been identified, involving different sources of nitrogen and carbon atoms. Structural, biochemical, and computational investigations have shed light on the mechanism of isonitrile formation through an unprecedented reaction by the enzyme ScoE.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Xiaojun Li, Shan Xue, Yisong Guo, Wei-Chen Chang
Summary: This study investigates a new pathway for C-C bond formation in the biosynthetic pathway of dehydrofosmidomycin, utilizing the mononuclear iron- and 2-oxoglutarate-dependent enzymes. The research reveals a two-step process involving C=C bond installation and subsequent C-C bond formation, leading to alkyl chain elongation and demethylation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Physical
Qian Wei, Hai-Chun Zeng, Yi Zou
Summary: This study identified two parallel and complex pathways of fungal dioxafenestrane sesquiterpenes from terrestrial and marine-derived fungi, revealing their differences in late-stage tailoring steps. It also discovered important enzymes and catalytic steps, providing valuable insights for natural product generation and metabolic enzymes.
Article
Environmental Sciences
Ming Yi, Qi Sheng, Zhenmei Lv, Huijie Lu
Summary: Atenolol, a widely prescribed beta-blocker, has been found in wastewater at concentrations up to 300 μg/L, posing risks to aquatic organisms. The efficient degrading microorganism Hydrogenophaga sp. YM1 and its biodegradation pathway have been identified in this study, indicating potential for complete conversion of atenolol with the help of acetate addition under aerobic conditions.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Oncology
Ali Vaziri-Gohar, Joel Cassel, Farheen S. Mohammed, Mehrdad Zarei, Jonathan J. Hue, Omid Hajihassani, Hallie J. Graor, Yellamelli V. V. Srikanth, Saadia A. Karim, Ata Abbas, Erin Prendergast, Vanessa Chen, Erryk S. Katayama, Katerina Dukleska, Imran Khokhar, Anthony Andren, Li Zhang, Chunying Wu, Bernadette Erokwu, Chris A. Flask, Mahsa Zarei, Rui Wang, Luke D. Rothermel, Andrea M. P. Romani, Jessica Bowers, Robert Getts, Curtis Tatsuoka, Jennifer P. Morton, Ilya Bederman, Henri Brunengraber, Costas A. Lyssiotis, Joseph M. Salvino, Jonathan R. Brody, Jordan M. Winter
Summary: This study reveals that nutrient-deprived conditions in the tumor microenvironment inhibit cancer cell viability, while wild-type isocitrate dehydrogenase 1 (wtIDH1) enables pancreatic cancer cells to adapt to these conditions. The study also shows the potent inhibitory effects of allosteric inhibitors of mutant IDH1 (mIDH1) on wtIDH1, and highlights the therapeutic potential of FDA-approved mIDH1 inhibitor ivosidenib (AG-120) in pancreatic cancer.
Article
Medicine, General & Internal
Enrico Franceschi, Dario De Biase, Vincenzo Di Nunno, Annalisa Pession, Alicia Tosoni, Lidia Gatto, Giovanni Tallini, Michela Visani, Raffaele Lodi, Stefania Bartolini, Alba Ariela Brandes
Summary: This study found that non-canonical IDH1 mutations may be associated with improved survival among patients with IDH1 mutated grade II-III glioma. These patients were typically younger and less likely to have 1p19q codeletion.
Article
Cell Biology
Wei Xu, Chirag H. Patel, Liang Zhao, Im-Hong Sun, Min-Hee Oh, Im-Meng Sun, Rachel S. Helms, Jiayu Wen, Jonathan D. Powell
Summary: GOT1 plays a crucial role in CD8+ T cell effector differentiation and function by maintaining redox balance and promoting purine nucleotide biosynthesis, glycolytic programming, and cytotoxic function, possibly through posttranslational regulation of HIF protein. Conversely, genetic deletion of GOT1 promotes the generation of memory CD8+ T cells.
Article
Oncology
Mark P. van Opijnen, C. Mircea S. Tesileanu, Linda Dirven, Pim B. van der Meer, Maarten M. J. Wijnenga, Arnaud J. P. E. Vincent, Marike L. D. Broekman, Hendrikus J. Dubbink, Johan M. Kros, Sjoerd G. van Duinen, Marion Smits, Pim J. French, Martin J. van den Bent, Martin J. B. Taphoorn, Johan A. F. Koekkoek
Summary: IDHwt hLGG patients and IDHwt glioblastoma patients have similar incidence proportions of epilepsy during the entire disease course. However, the former has a significantly higher incidence of epilepsy before diagnosis and a significantly longer time between first seizure and diagnosis, indicating a distinct clinical course between the two subtypes.
Article
Biochemistry & Molecular Biology
Kunhua Hu, Yufeng Ding, Hongwen Zhu, Xiaoqian Jing, Weiling He, Hua Yu, Xiongjun Wang
Summary: Tumor cells surviving hypoxic stress acquire the ability to drive cancer progression. Glutamate dehydrogenase 1 (GDH1) plays a critical role in regulating colorectal cancer (CRC) cell survival under hypoxia. GDH1 deficiency inhibits CRC occurrence and impairs hypoxia-inducible factor 1-alpha (HIF-1a) stability, promoting CRC progression through the modulation of GDH1 acetylation at K503 and K527.
Article
Biochemistry & Molecular Biology
Shuai Wang, Jingheng Wu, Wujun Zhao, Miaomiao Li, Shaoyi Li
Summary: CEBPB and P4HA2 are upregulated in IDH1 wt gliomas and associated with poor prognosis. CEBPE promotes proliferation and TMZ resistance by inducing P4HA2 expression in glioma cells.
Article
Chemistry, Multidisciplinary
Augustin Braun, Leland B. Gee, Michael W. Mara, Ethan A. Hill, Thomas Kroll, Dennis Nordlund, Dimosthenis Sokaras, Pieter Glatzel, Britt Hedman, Keith O. Hodgson, A. S. Borovik, Michael L. Baker, Edward I. Solomon
Summary: Fe K-edge XAS is widely used for studying high-valent iron intermediates in catalysts. The 4p-mixing into the 3d orbitals complicates the analysis, but understanding it correctly enables deeper insights into the structure and reactivity. This study reveals that the loss of inversion in the equatorial plane leads to 4p mixing into the 3dx2-y2,xy orbitals, providing structural insights for distinguishing 6- vs 5-coordinate active sites. Furthermore, the study investigates the electronic structure of Fe(IV)=O active sites and their reactivity selectivity through O K-edge XAS.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Michael A. Mingroni, Michael J. Knapp
Summary: FIH is a crucial oxygen sensing enzyme that hydroxylates a specific residue of HIF-1α under cellular hypoxia. Through studying the enzyme's kinetic properties and intermediate formation, the uncoupling of O2 activation by FIH was observed, providing new insights into the regulation of cellular oxygen homeostasis.
Article
Cell Biology
Taifu Gu, Ting Yang, Jianglong Huang, Jianhua Yu, Hongxin Ying, Xinlan Xiao
Summary: This study found that IVIM-DWI is effective in differentiating glioma grading and IDH1 mutation status. By comparing factors such as cerebral blood flow, perfusion fraction, and diffusivity, it can assist in making more accurate judgments in clinical diagnosis.
Article
Cell Biology
Yu Lei, Yuzhu Li, Lianchun Yu, Longzhou Xu, Xin Zhang, Gaoxing Zheng, Liang Chen, Wei Zhang, Xiaoying Qi, Yuxiang Gu, Yuguo Yu, Ying Mao
Summary: This study reveals the decayed critical dynamics of MMD in both the resting and task states for the first time, and proposes several EEG and fMRI features to identify its two subtypes.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2021)
Article
Cell Biology
Mengli Zhang, Huaichao Zhang, Minjie Fu, Jingwen Zhang, Cheng Zhang, Yingying Lv, Fengfeng Fan, Jinsen Zhang, Hao Xu, Dan Ye, Hui Yang, Wei Hua, Ying Mao
Summary: B7H3 expression is significantly decreased in IDH-mutated gliomas, possibly due to the accumulation of 2-HG and enhanced activity of the IDH1-R132H mutant. Inhibition of autophagy can block the degradation of B7H3 in glioma cells, while IDH1-R132H glioma cells exhibit a more active autophagy flux.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Oncology
Yan Yuan, Tianling Ding, Shu Wang, Hong Chen, Ying Mao, Tong Chen
Summary: PCNSL is a rare type of lymphoma primarily treated with high-dose methotrexate-based chemotherapy, stem cell transplantation, and whole-brain radiotherapy. Future treatments may focus on targeted therapy and personalized medicine.
BIOMARKER RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Zijing Wang, Zhipeng Yang, Jianjuan Jiang, Zhifeng Shi, Ying Mao, Nan Qin, Tiger H. Tao
Summary: A silk fibroin microneedle (SMN) patch designed for glioblastoma (GBM) can bypass the blood-brain barrier and release multiple drugs directly to the tumor site, providing an effective treatment strategy for brain tumors. The biocompatible and biodegradable patch allows for slow drug release over time and can be triggered remotely for rapid drug delivery. In animal models, the patch resulted in decreased tumor volume and increased survival rate, showing potential for clinical treatment of brain tumors.
ADVANCED MATERIALS
(2022)
Review
Developmental Biology
Lijuan Zhang, Yue Cao, Xin Zhang, Xinyang Gu, Ying Mao, Bo Peng
Summary: Microglia, as important immune cells in the central nervous system, play crucial roles in neurodevelopment, acute injury, and neuropsychiatric disorders. They undergo birth, death, and regeneration during their lifetime. Modulating the development of microglia may provide new therapeutic opportunities for preventing inflammation-related effects and controlling excessive inflammation in brain diseases.
DEVELOPMENTAL NEUROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ziyi Jin, Qi Yue, Wenjia Duan, An Sui, Botao Zhao, Yinhui Deng, Yuting Zhai, Yuwen Zhang, Tao Sun, Guang-Ping Zhang, Limei Han, Ying Mao, Jinhua Yu, Xiao-Yong Zhang, Cong Li
Summary: This article reports an intelligent surface-enhanced Raman scattering navigation system that can delineate the acidic margins of gliomas without using exogenous probes. The system uses a homemade deep learning model to process Raman spectra and quickly delineate the pH map of the tumor resection bed. Experimental results demonstrate a significant improvement in overall survival rate of animal models after surgery guided by this system.
Review
Neurosciences
Rui-Zhe Zheng, Zeng-Xin Qi, Zhe Wang, Ze-Yu Xu, Xue-Hai Wu, Ying Mao
Summary: Significant progress has been made in identifying and managing disorders of consciousness in patients with acquired brain injury. Advanced technology and tools have improved diagnostic accuracy, but there are still challenges in clinical treatment and a lack of effective management protocols.
NEUROSCIENCE BULLETIN
(2023)
Review
Neurosciences
Qiyuan Zhuang, Hui Yang, Ying Mao
Summary: Glioma, the most common and lethal primary brain tumor, is highly heterogeneous and its cell state, including glioma stem cells, microglia, neurons, and immune cells, play crucial roles in tumor initiation, progression, and response to therapy.
NEUROSCIENCE BULLETIN
(2023)
Article
Biochemistry & Molecular Biology
Yifan Yuan, Yang Yu, Yu Guo, Yinghua Chu, Jun Chang, Yicheng Hsu, Patrick Alexander Liebig, Ji Xiong, Wenwen Yu, Danyang Feng, Baofeng Yang, Liang Chen, He Wang, Qi Yue, Ying Mao
Summary: In this study, the diagnostic accuracy of combined CEST and MRS in predicting glioma infiltration was evaluated. The results showed that CEST and MRS were correlated with FET-PET, but the correlation between CEST and MRS was weaker. The combination of CEST/MRS predicted tumor infiltration more accurately than using CEST or MRS alone.
Review
Neurosciences
Lijuan Zhang, Yafei Wang, Taohui Liu, Ying Mao, Bo Peng
Summary: Microglia, as immune cells in the central nervous system, play a crucial role in monitoring the environment and protecting neurons to maintain brain homeostasis. In diseased brains, microglia are important mediators of neuroinflammation and have been extensively studied for their involvement in neurodegeneration. This review summarizes the multifunctional contributions of microglia to homeostasis and their potential as therapeutic targets in neurodegenerative diseases, with a particular focus on microglial replacement therapy.
NEUROSCIENCE BULLETIN
(2023)
Article
Computer Science, Information Systems
Renping Yu, Han Zhang, Xuehai Wu, Xuan Fei, Qing Yang, Zhiwei Ma, Zengxin Qi, Di Zang, Weijun Tang, Ying Mao, Dinggang Shen
Summary: This study aims to predict the outcome of unconscious acquired brain injury patients and differentiate consciousness levels using brain functional imaging and machine learning techniques. The researchers construct functional networks based on resting-state fMRI and use connection strengths as features for outcome prediction and consciousness level differentiation. The study achieves improved classification accuracy for consciousness levels (84.78%) and recovery outcome prediction (89.74%) compared to other methods, and identifies contributive connections across the entire brain. These findings provide potential biomarkers for understanding consciousness and developing diagnostic, prognostic, and therapeutic guidelines for ABI patients.
IEEE JOURNAL OF BIOMEDICAL AND HEALTH INFORMATICS
(2023)
Article
Multidisciplinary Sciences
Fushun Wang, Wei Wang, Simeng Gu, Dan Qi, Nathan A. Smith, Weiguo Peng, Wei Dong, Jiajin Yuan, Binbin Zhao, Ying Mao, Peng Cao, Qing Richard Lu, Lee A. Shapiro, S. Stephen Yi, Erxi Wu, Jason H. Huang
Summary: The researchers discovered two distinct astrocytic Ca2+ signals in the mouse barrel cortex, which play different roles in modulating sensory transmission during sleep and arousal states.
NATURE COMMUNICATIONS
(2023)
Correction
Neurosciences
Lijuan Zhang, Yafei Wang, Taohui Liu, Ying Mao, Bo Peng
NEUROSCIENCE BULLETIN
(2023)
Article
Physics, Multidisciplinary
Andrea Buccellato, Yasir Catal, Patrizia Bisiacchi, Di Zang, Federico Zilio, Zhe Wang, Zengxin Qi, Ruizhe Zheng, Zeyu Xu, Xuehai Wu, Alessandra Del Felice, Ying Mao, Georg Northoff
Summary: In this study, the use of permutation entropy (PE) is proposed to estimate time delays from neural time series, which is a more robust alternative to autocorrelation window (ACW). The validity of this approach is demonstrated on synthetic neural data and hd-EEG human data, suggesting its potential use for characterizing conscious states.
Article
Acoustics
Shaoyuan Yan, Jiajun Shou, Junjin Yu, Jianping Song, Ying Mao, Kailiang Xu
Summary: The study developed an ultrafast ultrasound vector Doppler (ultrafast UVD) method for small vasculature hemodynamic imaging by combining multiangle vector Doppler strategy and ultrafast sequencing. The validity of the technique was demonstrated through experiments on a rotational phantom, rat brain, human brain, and human spinal cord. Compared with the widely accepted accurate flow velocimetry technique ultrasound localization microscopy (ULM), the rat brain experiment showed that the average relative error (ARE) of the velocity magnitude estimated by ultrafast UVD was approximately 16.2%, with a root-mean-square error (RMSE) of the velocity direction of 26.7°. The results demonstrated that ultrafast UVD is a promising tool for accurate blood flow velocity measurement, especially for organs with vasculature typically exhibiting tendential alignment of vascular trees, such as the brain and spinal cord.
IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL
(2023)