Article
Microbiology
Pavithra Daulagala, Brian R. Mann, Kathy Leung, Eric H. Y. Lau, Louise Yung, Ruipeng Lei, Sarea I. N. Nizami, Joseph T. Wu, Susan S. Chiu, Rodney S. Daniels, Nicholas C. Wu, David Wentworth, Malik Peiris, Hui-Ling Yen
Summary: Immune imprinting shapes the anti-HA antibody landscape, but the evaluation of anti-HA and anti-NA antibody responses in individuals born within the same birth cohort has been limited. This study characterized NA antigenic variants and found age-dependent imprinting of both anti-HA and anti-NA antibodies. Including NA proteins in influenza vaccine preparations may enhance vaccine efficacy.
Article
Chemistry, Multidisciplinary
Nopawit Khamto, Lada Chaichuang, Puracheth Rithchumpon, Worrapong Phupong, Phuangthip Bhoopong, Suriya Tateing, Wilart Pompimon, Natthawat Semakul, Ni-orn Chomsri, Puttinan Meepowpan
Summary: This study synthesized thirty-three DMC derivatives and evaluated their cytotoxicity against six carcinoma cell lines in vitro, with most derivatives showing higher cytotoxicity than DMC. Derivatives with 4'-O-caproylation and 4'-O-methylation displayed the strongest cytotoxicity against specific cell lines. Structure-activity relationship analysis highlighted the importance of 2'-OH and the derivatization pattern of 4'-OH. Additionally, molecular docking simulation studies provided insights into the interactions of these bioactive compounds with cyclin-dependent kinase 2 (CDK2).
Article
Chemistry, Multidisciplinary
Haifang Du, Hanxiang Li, Ping Wu, Jinghua Xue, Yunshan Wu, Xiaoyi Wei, Bo Liu
Summary: In this study, a new C-methyl flavonoid, farrerol 7-O-beta-D-(6-O-galloyl) glucopyranoside, and 11 known flavonoids were isolated from the leaves of Cleistocalyx conspersipunctatus. The structures of these flavonoids were determined using spectroscopic investigation and electronic circular dichroism computation. Compound 1 showed stronger inhibition of alpha-glucosidase compared to the established inhibitor corosolic acid, suggesting its potential as a valid alpha-glucosidase inhibitor. Molecular docking was used to explore the interaction mechanism between compound 1 and alpha-glucosidase, and the biosynthetic pathway of these flavonoids in C. conspersipunctatus was proposed.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Applied
So-Yeun Woo, Ji Yeong Yang, HanGyeol Lee, Hyoung Jae Ahn, Yong Bok Lee, Sun Hee Do, Jeong Yoon Kim, Woo Duck Seo
Summary: Oat seedlings are environmentally friendly and contain active compounds with health benefits. The average total content of isolated compounds peaked at 4711.3 mg/100 g after 5 days of harvest, and the most active compounds 6 and 7 showed neuraminidase inhibitory effects, with IC50 values of 3.7 and 20.5 mu M.
Article
Immunology
Jeanette Linnea Tingstedt, Christine Stephen, Christian Risinger, Ola Blixt, Vithiagaran Gunalan, Isik Somuncu Johansen, Anders Fomsgaard, Charlotta Polacek, Ria Lassauniere
Summary: Neuraminidase (NA) is an attractive vaccine target as it plays important roles in virus entry and release. In this study, influenza DNA vaccine-induced NA-specific antibodies were evaluated for their inhibition of NA activity and epitope mapping. The antibodies were found to target critical sites of NA, including the enzymatic site and sialic binding site, inhibiting the catalytic activity of NA. New potential antigenic sites were also identified. These findings provide valuable information for rational vaccine design.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Trung Dien Nguyen, Hong Thi Nguyen, Yen Hai Hoang, Huy Thanh Tran, Nhung Tuyet-Thi Thai
Summary: Using Cleistocalyx operculatus bud extracts, silver nanoparticles were synthesized through a novel, eco-friendly, and low-priced process, which advances chemical and physical methods. The extracts played a dual role in reducing and stabilizing the nanoparticles. The obtained silver nanoparticles exhibited moderate stability and showed antibacterial and anticancer activity, with ethanol-water extract leading to higher activity than water extract.
Article
Microbiology
Meiling Dai, Wenjuan Du, Carles Martinez-Romero, Tim Leenders, Tom Wennekes, Guus F. Rimmelzwaan, Frank J. M. van Kuppeveld, Ron A. M. Fouchier, Adolfo Garcia-Sastre, Erik de Vries, Cornelis A. M. de Haan
Summary: Research shows that the antigenic and enzymatic properties of the influenza A virus neuraminidase are intertwined, with several residues affecting multiple properties. This entanglement may play a crucial role in the evolution of the neuraminidase.
Article
Pharmacology & Pharmacy
Jeremy C. Jones, Hui-Ling Yen, Peter Adams, Kimberly Armstrong, Elena A. Govorkova
Summary: Effective antivirals play a crucial role in the early phase of an influenza pandemic, before vaccines are available. Currently, two classes of antiviral drugs are approved for influenza treatment, but resistance has been observed. Therefore, it is important to develop novel antiviral options that target both the virus and the host. This review explores the drawbacks of current antivirals and ongoing research on new agents or combination therapies to address these limitations.
ANTIVIRAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Kristien Van Reeth, Anna Parys, Jose Carlos Mancera Gracia, Ivan Trus, Koen Chiers, Philip Meade, Sean Liu, Peter Palese, Florian Krammer, Elien Vandoorn
Summary: This study demonstrates that sequential vaccination with diverse H1N1 viruses in pigs induces a broad H1N1 antibody response, providing protection against multiple H1N1 strains in humans and pigs.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Lien T. M. Do, Jirapast Sichaem
Summary: This study provides new evidence for the medicinal potential of Melodorum fruticosum by isolating new flavonoid derivatives from its stem bark.
Article
Pharmacology & Pharmacy
Chia-Ying Wu, Shao-En Kao, Yung-Chieh Tseng, Yu-Po Lin, Jen-Tzu Hou, Li-Yang Wu, Sharon Chiu, Che Alex Ma, Pei-Wen Hsiao, Jane Hsiao, Juine-Ruey Chen
Summary: Influenza epidemics and pandemics caused by newly emerging virus strains call for the development of a universal vaccine. A monoglycosylated vaccine has been shown to provide broader protection in mice than conventional vaccines, but the challenge lies in developing a robust manufacturing process for mass production and verifying its immunogenicity in inducing cross-protective immunity.
ANTIVIRAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Xiuhua Lu, Zhu Guo, Zhu-Nan Li, Crystal Holiday, Feng Liu, Stacie Jefferson, F. Liaini Gross, Wen-Ping Tzeng, Anand Kumar, Ian A. York, Timothy M. Uyeki, Terrence Tumpey, James Stevens, Min Z. Levine
Summary: This study analyzed the antibody profiles of patients infected with A(H1N1)pdm09 viruses and found that although some adults produced high levels of antibodies, they still suffered from severe illness or death. The study suggests that narrowly-focused, low-quality antibody responses may contribute to severe lower respiratory tract infection.
SCIENTIFIC REPORTS
(2022)
Article
Virology
Sina Moeini, Atefeh Mohebbi, Behrokh Farahmand, Parvaneh Mehrbod, Fatemeh Fotouhi
Summary: The aim of this study was to analyze the neuraminidase (NA) segment of Iranian influenza A (H1N1) isolates and investigate their phylogenetic relationship, antiviral resistance, and vaccine efficiency. The phylogenetic study showed that most Iranian isolates from 2015-2016 were in a single clade, while subsequent years were in a subclade with major mutations. Drug-resistant mutations in the NA gene were detected in some isolates during the 2015-2016 flu seasons. The investigation of vaccine effect revealed the closest match to different strains in different years. This study provides valuable insights into the evolutionary trend of H1N1 influenza A virus in Iran.
Article
Virology
Alvaro Lopez-Valinas, Laura Baioni, Lorena Cordoba, Ayub Darji, Chiara Chiapponi, Joaquim Segales, Llilianne Ganges, Jose Nunez
Summary: Swine influenza viruses (SIV) cause a highly contagious disease that can lead to economic losses in the pig industry. Vaccination is commonly used to control SIV, but the presence of pre-existing immunity may affect the virus's evolutionary dynamics. This study analyzed the genomic variations in vaccinated and nonvaccinated pigs after a natural infection to understand the adaptability of SIV.
Article
Biochemistry & Molecular Biology
Garcia-Machorro Jazmin, Mirzaeicheshmeh Elaheh, Fragoso-Vazquez Manuel Jonathan, Bello Martiniano, Mendez-Luna David, Leon-Cardona Alam, Correa-Basurto Jose
Summary: Antiviral resistance is a global concern, particularly in the case of influenza A H1N1 which has developed resistance to oseltamivir and zanamivir. A new compound derived from oseltamivir was designed using computational methods and tested against influenza A H1N1. The compound showed significant affinity for both the neuraminidase (NA) and hemagglutinin (HA) proteins of the virus, as confirmed by in silico and in vitro assays, and it also demonstrated effectiveness in reducing viral susceptibility without any cytotoxic effects. The compound's high affinity for the viral NA enzyme, supported by molecular dynamics simulations, suggests its potential as an antiviral against influenza A H1N1.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Plant Sciences
Thi-Phuong Doan, Eun-Jin Park, Hyo-Moon Cho, Byeol Ryu, Ba-Wool Lee, Phuong-Thien Thuong, Won-Keun Oh
Summary: Rugonidines A-F, isolated from Alchornea rugosa, are novel configurationally semistable diastereomers featuring a unique scaffold. Their structures were elucidated through NMR spectroscopy and quantum-chemical calculations. These compounds showed a significant increase in glucose uptake in differentiated 3T3-L1 adipocytes.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Plant Sciences
Hyo-Moon Cho, Mi Zhang, Eun-Jin Park, Ba-Wool Lee, Yeon-Joo Park, Hyun-Woo Kim, Ha-Thanh-Tung Pham, Young-Won Chin, Won-Keun Oh
Summary: This study discovered several flavonostilbenes from the stems of Rhamnoneuron balansae, and evaluated their effects on SIRT1 deacetylase activity. One compound showed promising anti-aging activity in vitro.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Plant Sciences
Trong-Tuan Dao, Katherine Williams, Kate M. J. de Mattos-Shipley, Zhongshu Song, Yuiko Takebayashi, Thomas J. Simpson, James Spencer, Andrew M. Bailey, Christine L. Willis
Summary: Three new polyketide-derived natural products and six known metabolites were isolated from fermentation of the fungus Cladobotryum sp. Their antibacterial activities against Staphylococcus aureus strains revealed the importance of unsaturated decalins for biological activity.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Plant Sciences
Huong Pham, Thi Phuong Doan, Hyun Woo Kim, Tae Wan Kim, So-Yeon Park, Hangnun Kim, Mina Lee, Ki Hyun Kim, Won Keun Oh, Young Woon Lim, Kyo Bin Kang
Summary: Microbial cocultivation is a strategy to induce the biosynthesis of specialized metabolites. In this study, the coculture of Phellinus orientoasiaticus and Xylodon flaviporus was found to induce the production of multiple metabolites. The isolated compounds did not exhibit antifungal activity or cytotoxicity.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Chemistry, Organic
Joonseok Jang, Jinwoo Lee, Seok Beom Lee, Seung Hyun Choi, Eun Jin Park, Sang Jun Yoon, Joon Soo An, Dong-Chan Oh, Won Keun Oh, Suckchang Hong
Summary: In this study, we reported the total synthesis of melicoptelines C-E, which exhibited anti-influenza activity. The core 3a-hydroxy HPIs were synthesized and then coupled and cyclized to obtain the target compounds. Among them, melicopteline E showed the most potent inhibition of cytopathic effects.
Article
Chemistry, Medicinal
Byeol Ryu, Eun-Jin Park, Thi-Phuong Doan, Hyo-Moon Cho, Jin-Pyo An, Thi-Linh-Giang Pham, Ha-Thanh-Tung Pham, Won-Keun Oh
Summary: Ten phenolic constituents, including three new macrocyclic glycosides and a new phenolic glycoside, were isolated from the leaves of Heliciopsis terminalis. These compounds showed stimulatory effects on glucose uptake and some of them activated the AMPK signaling pathway.
Article
Chemistry, Medicinal
Hee-Ju Lee, Eun-Jin Park, Byeol Ryu, Hyo-Moon Cho, Duc-Trong Nghiem, Ha-Thanh-Tung Pham, Cheol-Ho Pan, Won-Keun Oh
Summary: This study isolated new benzylisoquinoline alkaloid derivatives from Limacia scandens and tested their autophagy-regulating activity. Several compounds showed potential autophagy inhibition activity, providing potential candidates for developing natural autophagy inhibitors.
Article
Biochemistry & Molecular Biology
Ha-Thanh-Tung Pham, Eun-Jin Park, Byeol Ryu, Hee-Ju Lee, Thi-Phuong Doan, Hyo-Moon Cho, Thi-Linh-Giang Pham, Won-Keun Oh
Summary: Chemical investigation of Gymnema latifolium led to the discovery of seven undescribed 23-glycosyl oleanane triterpenoids, gymlatinosides GLF1-GLF7, and two known compounds, gymnemosides D and E. The structures of these compounds were determined using various spectroscopic methods. The extract of G. latifolium and all isolated compounds significantly increased glucose uptake in 3T3-L1 adipocytes at a concentration of 20 μM. Among them, gymlatinosides GLF2 and gymlatinosides GLF4 exhibited particularly potent dose-dependent effects on glucose uptake. Further investigation revealed that gymlatinosides GLF2 upregulated the expression of phosphorylated AMPK (p-AMPK) at a concentration of 20 μM. These findings suggested that gymlatinosides GLF2 may enhance glucose uptake by regulating the AMPK signaling pathway.
Article
Biochemistry & Molecular Biology
Thi-Phuong Doan, Eun-Jin Park, Byeol Ryu, Hyo-Moon Cho, Sang -Jun Yoon, Gwan-Young Jung, Phuong-Thien Thuong, Won-Keun Oh
Summary: A phytochemical investigation of Alchornea rugosa leaves revealed eight undescribed guanidine-flavanol conjugates named rugonines A-H. The chemical structures of these compounds were comprehensively elucidated using NMR spectroscopy, HRESIMS, and circular dichroism (CD) analysis. In autophagosome formation experiments conducted in HEK293 cells expressing GFP-LC3, rugonines D-G showed potential autophagy inhibitory activity.
Article
Agriculture, Dairy & Animal Science
Eun Jin Park, Ba Wool Lee, Byeol Ryu, Hyo Moon Cho, Seong Kyun Kim, Seong-Sik Yoo, Eunhee Kim, Won Keun Oh
Summary: This study aimed to discover an anti-influenza herbal mixture and evaluate its antiviral activity against avian influenza. Three different medicinal plants with distinct medicinal purposes were selected and formulated into a combination, which showed potent inhibitory effects on the virus and reduced mortality and virus titers in chickens infected with avian influenza.
Article
Environmental Sciences
Hyeon-Jeong Lee, Minsik Kang, Junghyun Son, Hophil Min, Sangwon Cha, Han Bin Oh, Won Keun Oh, Jaeick Lee
Summary: Phenols are important environmental endocrine disruptors with potential adverse health effects. A comprehensive and sensitive analytical method for multiple classes of phenols was developed by investigating the influence of mobile phase compositions in LC-ESI-MS/MS analysis. This method allowed for the concurrent ionization of all phenols and significantly improved the analytical sensitivity for certain phenols.
ENVIRONMENTAL RESEARCH
(2023)
Article
Plant Sciences
Thi-Phuong Doan, Mi Zhang, Eun-Jin Park, Jorge-Eduardo Ponce-Zea, Van-Hieu Mai, Hyo-Moon Cho, Ha-Thanh-Tung Pham, Won-Keun Oh
Summary: This study investigated the chemical components of avocado seeds and discovered several new alkaloids. The bioactivities of these compounds on SIRT1 were evaluated, and one compound showed the most potent activation effect, suggesting its potential as an anti-aging agent.
JOURNAL OF NATURAL PRODUCTS
(2023)
Correction
Plant Sciences
Huong T. Pham, Thi Phuong Doan, Hyun Woo Kim, Tae Wan Kim, So-Yeon Park, Hangun Kim, Mina Lee, Ki Hyun Kim, Won Keun Oh, Young Woon Lim, Kyo Bin Kang
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Hee Ju Lee, Mi Zhang, Thi Phuong Doan, Eun-Jin Park, Duc-Trong Nghiem, Ha-Thanh-Tung Pham, Cheol-Ho Pan, Won-Keun Oh
Summary: Seven undescribed chemicals, including two syringylglycerol derivatives, two cyclopeptides, one tigliane analogue, and two chromone derivatives, were isolated from the stems of Limacia scandens during the screening of senotherapeutics from natural products. The structures of these compounds were determined through spectroscopic data analysis. Testing these compounds in replicative senescent human dermal fibroblasts revealed that one tigliane and two chromone derivatives exhibited senolytic activity, selectively eliminating senescent cells. In particular, 2-{2-[(3'-O-beta-D-glucopyranosyl) phenyl]ethyl}chromone showed potential as a senotherapeutic agent by inducing HDF death, inhibiting the activity of senescence-associated beta-galactosidase (SA-beta-gal), and expressing senescence-associated secretory phenotype (SASP) factors.
Article
Biochemistry & Molecular Biology
Byeol Ryu, Eun-Jin Park, Thi-Phuong Doan, Hyo-Moon Cho, Jorge-Eduardo Ponce-Zea, Van-Hieu Mai, Vahideh Oveissi, Won-Keun Oh
Summary: Autophagy inhibitory activity was found in unique oligomeric flavonoids derived from the roots of Lindera erythrocarpa. The structures and configurations of these compounds were determined using various techniques. The study revealed that these compounds can inhibit autophagy by blocking the fusion process between autophagosome and lysosome.
