Journal
JOURNAL OF NATURAL MEDICINES
Volume 69, Issue 2, Pages 171-177Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s11418-014-0874-4
Keywords
Anti-complement; Entada phaseoloides; Intestinal bacterial; Metabolites
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Funding
- National Project in Significant Creation of New Drugs [2012ZX09103201-038]
- National Natural Science Foundation of China [81173551, 81274062]
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The present study aimed to investigate the metabolism of Entadae Semen by human fecal bacteria to clarify the relationship between its pharmacological activities and intestinal metabolism. Three major components (phaseoloidin, entadamide A-beta-d-glucopyranoside and entadamide A) were isolated and identified from Entadae Semen and then incubated with human fecal microflora in vitro to investigate the metabolic processes. The metabolites were analyzed with high-performance liquid chromatography (HPLC). The anti-complement activities of the three components and their metabolites produced by human fecal microflora were evaluated in vitro using a hemolysis assay. Phaseoloidin and entadamide A-beta-d-glucopyranoside were metabolized into their respective aglycones during the incubation process, which enhanced their anti-complement effects. These results indicated that the presence of intestinal bacteria likely plays an important role and that the pharmacological effects of Entadae Semen may be dependent on intestinal bacterial metabolism.
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