Journal
JOURNAL OF NATURAL MEDICINES
Volume 63, Issue 1, Pages 75-79Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s11418-008-0283-7
Keywords
Chloroplast DNA; C; aromatica; C; longa; C; xanthorrhiza; C; zedoaria
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Funding
- Japan Kampo Medicine Manufacturers Association
- Ministry of Education, Culture, Sports, Science and Technology
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With the goal of developing an accurate plant identification method, molecular analysis based on polymorphisms of the nucleotide sequence of chloroplast DNA (cpDNA) was performed in order to distinguish four Curcuma species: C. longa, C. aromatica, C. zedoaria, and C. xanthorrhiza. Nineteen regions of cpDNA were amplified successfully via polymerase chain reaction (PCR) using total DNA of all Curcuma plants. Using the intergenic spacer between trnS and trnfM (trnSfM), all four Curcuma plant species were correctly identified. In addition, the number of AT repeats in the trnSfM region was predictive of the curcumin content in the rhizome of C. longa.
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