Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 13, Issue 1, Pages 603-606Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2013.6926
Keywords
Magnetic Nanoparticles (MNPs); Fe-AuNPs/PHEMA; ATRP; Protein Immobilization
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Funding
- Joint Program of Cooperation in Science and Technology through NRF
- MEST [2011-0025680]
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Biocompatible magnetic nanoconnposites of Fe-AuNPs and poly(2-hydroxylethyl methacrylate) (PHEMA) were employed as a strategic protein immobilization platform. The hybrid magnetic nanocomposites were prepared by applying a 'grafting to' ATRP protocol. Fe-AuNPs having Fe core and Au shell were initially prepared by the inverse micelle method. Disulfide-containing PHEMA (DT-PHEMA) was grafted to the Fe-AuNPs surface by taking the advantages of the thiol chemistry. The grafting of DT-PHEMA to the Fe-AuNPs was confirmed by relevant spectroscopic analyses. The superparamagnetic property, a basic requirement for facile protein immobilization, of the magnetic nanocomposites was measured by the SQUID analysis. Lysozyme, gamma-globulins and bovine serum albumin (BSA) were immobilized onto magnetic nanocomposites via the adsorption strategy. The absorption intensity of lysozyme, gamma-globulins and BSA on the PHEMA grafted Fe-AuNPs were observed to be higher than that of bare Fe-AuNPs.
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