Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 11, Issue 6, Pages 5223-5227Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2011.4129
Keywords
Magnetic Nanoparticles; Gold Nanoparticles; Carboxymethyl Chitosan; HRP
Categories
Funding
- National Natural Science Foundation of China [21074152]
- 863 project [2007AA022007]
- University Creative Group Project of Hunan Education Department
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A novel enzyme support based on magnetic/polymer/nanogold complex was prepared in this study. At first, carboxymethyl chitosan (CMC) were connected onto the surface of Fe3O4 nanoparticles, and then gold nanoparticles were in situ synthesized and bound to CMC chains, resulting in Fe3O4/CMC/nanogold complex. The complex was determined by UV-vis spectroscopy, transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). Horseradish peroxidase (HRP) was immobilized on the Fe3O4/CMC/nanogold complex. Compared with free HRP, the immobilized HRP exhibits higher catalytic activity and better storage stability. This Fe3O4/CMC/nanogold complex can serve as an excellent support for enzymes.
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