Journal
JOURNAL OF NANOPARTICLE RESEARCH
Volume 14, Issue 2, Pages -Publisher
SPRINGER
DOI: 10.1007/s11051-011-0680-9
Keywords
Silica; Alexa dyes; Fluorescent particles; Quantum yields; Nanoparticles; Protective shell; Nanobiotechnology
Categories
Funding
- Federal Institute for Materials Research and Testing (BAM)
- Federal Ministry of Economics and Technology [BMWI-22/06]
Ask authors/readers for more resources
Current and future developments in the emerging field of nanobiotechnology are closely linked to the rational design of novel fluorescent nanomaterials, e. g. for biosensing and imaging applications. Here, the synthesis of bright near infrared (NIR)-emissive nanoparticles based on the grafting of silica nanoparticles (SNPs) with 3-aminopropyl triethoxysilane (APTES) followed by covalent attachment of Alexa dyes and their subsequent shielding by an additional silica shell are presented. These nanoparticles were investigated by dynamic light scattering (DLS), transmission electron microscopy (TEM) and fluorescence spectroscopy. TEM studies revealed the monodispersity of the initially prepared and fluorophore-labelled silica particles and the subsequent formation of raspberry-like structures after addition of a silica precursor. Measurements of absolute fluorescence quantum yields of these scattering particle suspensions with an integrating sphere setup demonstrated the influence of dye labelling density-dependent fluorophore aggregation on the signaling behaviour of such nanoparticles.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available