Article
Multidisciplinary Sciences
Zheng Fang Yang, Pankaj Panwar, Ciaran R. McFarlane, Wietske E. Tuinte, Marta Campiglio, Filip Van Petegem
Summary: Junctophilins (JPH) are proteins that connect the plasma membrane and the endoplasmic or sarcoplasmic reticulum, facilitating communication between proteins in different membranes. This study presents crystal structures of two JPH isoforms, revealing their importance in muscle excitation-contraction coupling. Various mutations in JPH2 and the L-type calcium channel affect their interaction and have been linked to cardiomyopathy and cardiac arrhythmia, respectively.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Sansrity Sinha, Yael Elbaz-Alon, Ori Avinoam
Summary: In the process of muscle regeneration, cytosolic calcium plays a crucial role in coordinating differentiation, fusion, and contraction, which helps to avoid adverse consequences.
Review
Physiology
Susan Wray, Sarah Arrowsmith
Summary: This article reviews the advances in the understanding of myometrial physiology, focusing on excitation and the effects of gestation on ion channels. It discusses the role of various ion channels in myometrium, their modulation by key hormones, and their integration throughout gestation.
ANNUAL REVIEW OF PHYSIOLOGY, VOL 83
(2021)
Article
Cell Biology
Blerina Ahmetaj-Shala, Isra Marei, Ryota Kawai, Stephen Rothery, Charis Pericleous, Nura A. Mohamed, Hime Gashaw, Kalliopi Bokea, Jake Samuel, Annabelle Vandenheste, Fisnik Shala, Nicholas S. Kirkby, Jane A. Mitchell
Summary: Our study demonstrates that BO-SMCs exhibit functional responses similar to VSMCs when exposed to the thromboxane mimetic U46619, providing critical evidence for their application in biomedical research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zoltan Singlar, Peter Szentesi, Janos Fodor, Agnes Angyal, Laszlo Csernoch, Monika Sztretye
Summary: This study investigated the effects of nutraceutical supplementation in aging mice and found that AX diet limited weight gain without muscle tissue mass decline, while both AX and krill oil supplementation increased force production without affecting calcium homeostasis. Additionally, krill oil supplementation improved spatial memory and learning ability in aging mice, suggesting that antioxidant nutraceuticals can be beneficial for cognition and healthy aging.
Article
Biology
Xueyong Wang, Murad Nawaz, Chris DuPont, Jessica H. Myers, Steve Ra Burke, Roger A. Bannister, Brent D. Foy, Andrew A. Voss, Mark M. Rich
Summary: Excitation-contraction coupling is the process of converting electrical excitation of muscle into force generation. Depolarization of skeletal muscle can lead to failure of ECC, specifically the failure to generate calcium transients. Different AP properties, such as AP peak and AP integral, play important roles in different steps of ECC, including AP conduction and calcium release.
Article
Multidisciplinary Sciences
Melissa J. S. MacEwen, Domnita-Valeria Rusnac, Henok Ermias, Timothy M. Locke, Hayden E. Gizinski, Joseph P. Dexter, Yasemin Sancak
Summary: This study investigates the important role of myosin light chain kinase (MLCK) in cellular functions such as smooth muscle contraction and motility, which is activated by calcium ions (Ca2+) and calmodulin (CaM). Despite controversy, this research proposes a partially ordered mechanism for the binding of CaM to MLCK based on mathematical models and experimental data. These findings contribute to a better understanding of MLCK activation and provide insights into cellular processes and diseases.
Article
Biochemistry & Molecular Biology
Enrico Pierantozzi, Peter Szentesi, Cecilia Paolini, Beatrix Dienes, Janos Fodor, Tamas Olah, Barbara Colombini, Dilson E. Rassier, Egidio Maria Rubino, Stephan Lange, Daniela Rossi, Laszlo Csernoch, Maria Angela Bagni, Carlo Reggiani, Vincenzo Sorrentino
Summary: Obscurin plays a significant role in skeletal muscle fibers, affecting muscle function and intracellular Ca2+ dynamics. The diaphragm of Obscn KO mice show prolonged TTP and HRT, decreased sarcoplasmic reticulum Ca2+ release and SERCA reuptake rates, as well as fiber damage, altered sarcomere ultrastructure and M-band disarray after intense exercise.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Malcolm J. Jackson, Natalie Pollock, Caroline Staunton, Samantha Jones, Anne McArdle
Summary: Research has shown that reactive oxygen species are generated in skeletal muscle during exercise and play important roles in muscle adaptations. This review aims to summarize the current understanding of the redox signaling roles of these species in mediating muscle responses to contractile activity, with a focus on the effects of aging. Additionally, evidence is provided suggesting that disruption of muscle mitochondria's redox status due to age-associated denervation may contribute to a weakening of muscle responses to contractile activity.
Article
Surgery
Xiao-Rong Wang, Hui-Bo Du, Huai-Huai Wang, Li-Min Zhang, Yong-Hua Si, Hong Zhang, Zi-Gang Zhao
Summary: This study investigated the impact of mesenteric lymph drainage on improving cardiac contractile dysfunction induced by post-hemorrhagic shock mesenteric lymph return. The results showed that mesenteric lymph drainage can restore cardiomyocyte calcium sensitivity and improve cardiac contractile response. These findings suggest that increased cardiac contractibility may be associated with the restoration of calcium sensitivity.
JOURNAL OF SURGICAL RESEARCH
(2021)
Article
Medicine, Research & Experimental
Jussep Salgado-Almario, Manuel Vicente, Yillcer Molina, Antonio Martinez-Sielva, Pierre Vincent, Beatriz Domingo, Juan Llopis
Summary: This study used zebrafish larvae as the research subject and employed imaging techniques to observe the contraction and calcium levels in the heart. The experimental results showed that this method does not affect cardiac function and can detect changes in calcium levels at different time points. In addition, the study validated the effects of certain drugs on cardiomyocytes. This research method can contribute to the study of pathophysiological mechanisms in heart diseases and drug screening.
Article
Cell Biology
Audrei R. Santos, Tatiana E. Koike, Alana M. Santana, Natalya C. Miranda, Rodrigo A. Dell Aquila, Thiago C. Silva, Marcelo S. Aoki, Elen H. Miyabara
Summary: The aim of this study was to investigate the effect of glutamine supplementation on the expression of HSP70 and calcium-binding proteins in recovering EDL muscle after injury. Glutamine supplementation increased myofiber size and prevented decline in muscle strength. It also upregulated myogenin expression, HSP70 expression, and attenuated the increase in pro-inflammatory cytokines and calcium-binding proteins. Overall, glutamine supplementation accelerated the recovery of muscle size and function by modulating gene expression.
HISTOCHEMISTRY AND CELL BIOLOGY
(2023)
Article
Anesthesiology
Lourdes Figueroa, Natalia Kraeva, Carlo Manno, Carlos A. Ibarra-Moreno, Eshwar R. Tammineni, Sheila Riazi, Eduardo Rios
Summary: By measuring sensitivity to halothane of myotubes and extending clinical observations and cell-level studies to a large group of patients, new insights into the pathological mechanism of malignant hyperthermia susceptibility have been obtained. Enhanced sensitivity to depolarisation and to halothane appear to be the primary, mutually reinforcing and phenotype-defining defects of halothane-hypersensitive patients with malignant hyperthermia susceptibility.
BRITISH JOURNAL OF ANAESTHESIA
(2023)
Article
Neurosciences
Valerie Biancalana, John Rendu, Annabelle Chaussenot, Helen Mecili, Eric Bieth, Melanie Fradin, Sandra Mercier, Maud Michaud, Marie-Christine Nougues, Laurent Pasquier, Sabrina Sacconi, Norma B. Romero, Pascale Marcorelles, Francois Jerome Authier, Antoinette Gelot Bernabe, Emmanuelle Uro-Coste, Claude Cances, Bertrand Isidor, Armelle Magot, Marie-Christine Minot-Myhie, Yann Pereon, Julie Perrier-Boeswillwald, Gilles Bretaudeau, Nicolas Dondaine, Alison Bouzenard, Megane Pizzimenti, Bruno Eymard, Ana Ferreiro, Jocelyn Laporte, Julien Faure, Johann Bohm
Summary: The recurrent RYR1 mutation previously classified as VUS was found in thirteen patients from nine unrelated congenital myopathy families with consistent clinical presentation, providing evidence of its pathogenicity. This mutation causes an atypical congenital myopathy with slowly improving motor function over the first decades of life, indicating a potential for directing molecular diagnosis for patients with similar clinical presentation.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Chih-Hsuan Chou, Elisabeth R. Barton
Summary: Glucose is a vital energy source for active skeletal muscles, and AMPK plays a crucial role in glucose uptake during muscle contraction. The study aimed to investigate the impact of increased muscle IGF-I on AMPK α phosphorylation and activity. The results demonstrated that high muscle IGF-I enhances glucose uptake under resting conditions but does not inhibit AMPK activity during muscle contraction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)