Journal
JOURNAL OF MOLECULAR RECOGNITION
Volume 25, Issue 3, Pages 165-173Publisher
WILEY
DOI: 10.1002/jmr.2179
Keywords
proximity energies; high concentration solutions; molecular crowding
Categories
Funding
- Biomolecular Interaction Technologies Center
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A high-concentration fluid may be defined as one in which the distance between the van der Waals surfaces of the molecules is on the same order of magnitude as the size of the molecules. Many biological fluids meet this definition of being concentrated. High concentration protein solutions are of commercial interest, too. In the biopharmaceutical industry, there is a desire to produce high concentration (>100?mg/ml) protein formulations. The goal of this paper is to provide an energetic framework for understanding the functional properties (e.g., solubility, stability and viscosity) of high concentration solutions. Using this framework not only provides a simple, clear view of protein behavior at high concentrations, it also provides insights into how modifications to the protein or the solvent affect solution behavior. Thus, the paper provides a starting point for the rational manipulation of protein solubility, viscosity and stability. Copyright (c) 2012 John Wiley & Sons, Ltd.
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