Article
Endocrinology & Metabolism
Amanda Stenzel, Abigail Mumme-Monheit, Juliana Sucharov, Macie Walker, Jennyfer M. Mitchell, Bruce Appel, James T. Nichols
Summary: The Notch pathway is critical for craniofacial development, with the her9 gene essential for bone mineralization and working redundantly with her6 in certain craniofacial patterning processes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Gota Yoshida, Tsuyoshi Kawabata, Hyota Takamatsu, Shotaro Saita, Shuhei Nakamura, Keizo Nishikawa, Mari Fujiwara, Yusuke Enokidani, Tadashi Yamamuro, Keisuke Tabata, Maho Hamasaki, Masaru Ishii, Atsushi Kumanogoh, Tamotsu Yoshimori
Summary: This study found that upregulation of autophagy in osteoblasts promotes bone formation and deficiency in autophagy disrupts bone homeostasis. It identified RUBCN as a key regulator of bone homeostasis and suggested that targeting RUBCN or autophagic degradation of NICD could be used as potential treatments for osteoporosis and bone fractures.
Article
Cell Biology
Thomas H. Shin, Evangelos Theodorou, Carl Holland, Rae'e Yamin, Cathleen L. Raggio, Philip F. Giampietro, David A. Sweetser
Summary: This study identified a previously unrecognized role of Tle4 in bone calcification and investigated its impact on osteoblast function. Loss of Tle4 was shown to adversely affect bone development, potentially through regulation of key osteoblastogenic genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kyung-Ran Park, Joon Yeop Lee, Myounglae Cho, Jin Tae Hong, Hyung-Mun Yun
Summary: Paeonoside (PASI) isolated from Paeonia suffruticosa roots enhances osteoblast migration, early differentiation, and mineralized nodule formation by regulating RUNX2 expression through the BMP2 and Wnt3a pathways, suggesting a potential therapeutic role in treating bone diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Tomoka Hasegawa, Hiromi Hongo, Tomomaya Yamamoto, Miki Abe, Hirona Yoshino, Mai Haraguchi-Kitakamae, Hotaka Ishizu, Tomohiro Shimizu, Norimasa Iwasaki, Norio Amizuka
Summary: Bone mineralization involves primary and secondary mineralization phases, with osteoblasts secreting matrix vesicles during primary mineralization, and subsequent increase in bone mineral density during secondary mineralization. Osteocytes may play a key role in this process through the transport of calcium and phosphate ions and regulation of mineralization of the bone matrix.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Parisa Dashti, Jeroen van de Peppel, Roman Thaler, Christopher R. Paradise, Gary S. Stein, Martin A. Montecino, Johannes P. T. M. van Leeuwen, Bram J. van der Eerden, Amel Dudakovic, Andre J. van Wijnen
Summary: This study found that Smyd2 inhibits proliferation and mineral deposition by osteoblasts, and operates parallel to the suppressive effects of Ezh2 and H3K27 trimethylation on osteoblast differentiation.
Article
Cell Biology
Irina L. Tourkova, Quitterie C. Larrouture, Kelechi M. Onwuka, Silvia Liu, Jianhua Luo, Paul H. Schlesinger, Harry C. Blair
Summary: This study showed for the first time that specific enzymes involved in bone mineral transport and matrix synthesis proteins in the epithelial-like bone-forming cell layer are downregulated with aging. Results were compared using cells extracted from long bones of young and old mice, or in essentially uniform osteoblasts differentiated from stromal stem cells in vitro. The age effect showed memory in the stromal stem cells, which is a remarkable finding.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ke Wang, Yinshi Ren, Shuxian Lin, Yan Jing, Chi Ma, Jun Wang, X. Baozhi Yuan, Xianglong Han, Hu Zhao, Zheng Wang, Minghao Zheng, Yin Xiao, Lin Chen, Bjorn Reino Olsen, Jian Q. Feng
Summary: This study found that osteocytes, not osteoblasts, directly build mineralized bone structures, and that osteoblasts in Dmp1-null or Hyp mice produce aberrant and poorly mineralized bone. Additionally, the constitutive expression of beta-catenin in osteocytes can recapitulate a similar osteomalacia phenotype as seen in Dmp1 null or Hyp mice. Thus, the conclusion is that osteocytes directly build bone, challenging the existing dogma.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Hsiao-Cheng Tsai, Che-Hong Chen, Daria Mochly-Rosen, Yi-Chen Ethan Li, Min-Huey Chen
Summary: The research indicates that the ALDH2*2 mutation combined with alcohol consumption and chronic exposure to bacterial toxins from periodontal disease increases the risk of dental bone loss. Experimental results show that ALDH2 knockdown leads to loss of proliferation and mineralization capability in osteoblasts.
Article
Biochemistry & Molecular Biology
Norihisa Uehara, Nobuhide Shibusawa, Yoshikazu Mikami, Yukari Kyumoto-Nakamura, Soichiro Sonoda, Hiroki Kato, Takayoshi Yamaza, Toshio Kukita
Summary: The study found that extracellular vesicles (EVs) produced by breast cancer cells inhibit osteoblast differentiation and functions by regulating JNK activity, providing new insights into the pathological impact of osteolytic bone metastasis and the role of EVs in osteoblast differentiation.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Yongwei Du, Gongwen Liu, Zhen Liu, Jianwen Mo, Miao Zheng, Qi Wei, Youjia Xu
Summary: Avermectin, a widely used insecticide, has been found to have significant toxic effects on bone development in zebrafish embryos. It disrupts early bone development, causes abnormal craniofacial chondrogenesis, and reduces bone mineralization. Avermectin also decreases the expression of genes related to osteogenesis and TGF-beta and BMP signaling pathways. The study provides insight into the mechanism of avermectin-induced damage on bone development and demonstrates its toxicity.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Nutrition & Dietetics
Letizia Pelusi, Domitilla Mandatori, Nadia Di Pietrantonio, Francesco Del Pizzo, Pamela Di Tomo, Natalia Di Pietro, Roberto Buda, Salvatore Genovese, Francesco Epifano, Assunta Pandolfi, Serena Fiorito, Caterina Pipino
Summary: Bone physiology is regulated by osteoblast and osteoclast activities. This study demonstrates that the coumarin derivative umbelliferon (UF) enhances the activity of osteoblasts from osteoporotic patients through estrogen receptor 1 (ESR1) and the activation of the beta-catenin pathway. UF also reduces the multinucleated cells involved in bone resorption. These findings suggest that UF may be a potential natural compound for the prevention and treatment of bone loss diseases.
Article
Agriculture, Multidisciplinary
Gong-Jhe Wu, Kung-Yen Chen, Jr-Di Yang, Shing-Hwa Liu, Ruei-Ming Chen
Summary: The study showed that naringin, a citrus flavonoid, can enhance bone mineralization and healing in osteoporosis-related bone diseases by activating ERα and ALP expression.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Jerry Maria Sojan, Ratish Raman, Marc Muller, Oliana Carnevali, Jorg Renn
Summary: Zebrafish larvae are increasingly used to study vertebrate skeletal development and human pathologies. Recent research has discovered that probiotics can improve bone health. By using zebrafish transgenic lines, it was found that exposure to two different probiotics can increase osteoblast formation and bone matrix growth and mineralization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physiology
Joana T. Rosa, Paul Eckhard Witten, Ann Huysseune
Summary: The translation discusses the relationship between osteoblasts and odontoblasts, as well as the diverse tooth attachment modes and tissue properties in mammals and teleost fish. There is a debate about the nature of the bone of attachment tissue and the cells secreting it, with scpp5 expression helping to characterize the cells involved in the process. The findings suggest that the bone of attachment in zebrafish shares characteristics of both bone and dentin, and may be better termed as dentinous bone.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Giuseppe Banfi, Marco Diani, Paolo D. Pigatto, Eva Reali
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Clinical Neurology
Jacopo A. Vitale, Matteo Briguglio, Roberta Galentino, Bernardo Dell'Osso, Antonio Malgaroli, Giuseppe Banfi, Mauro Porta
JOURNAL OF AFFECTIVE DISORDERS
(2020)
Article
Infectious Diseases
Luca Pirisi, Federico Pennestri, Marco Vigano, Giuseppe Banfi
BMC INFECTIOUS DISEASES
(2020)
Article
Medical Laboratory Technology
Davide Ferrari, Andrea Motta, Marta Strollo, Giuseppe Banfi, Massimo Locatelli
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2020)
Article
Clinical Neurology
Andrea Luca, Riccardo Giorgino, Loreto Gesualdo, Giuseppe M. Peretti, Anas Belkhou, Giuseppe Banfi, Giovanni Grasso
WORLD NEUROSURGERY
(2020)
Article
Environmental Sciences
Francesco Magro, Paolo Perazzo, Elena Bottinelli, Francesco Possenti, Giuseppe Banfi
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2020)
Article
Physiology
Matteo Briguglio, Alberto Bona, Mauro Porta, Bernardo Dell'Osso, Fabrizio Ernesto Pregliasco, Giuseppe Banfi
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Medical Laboratory Technology
Federico Cabitza, Andrea Campagner, Davide Ferrari, Chiara Di Resta, Daniele Ceriotti, Eleonora Sabetta, Alessandra Colombini, Elena De Vecchi, Giuseppe Banfi, Massimo Locatelli, Anna Carobene
Summary: The study found that machine learning models can identify COVID-19 positive patients quickly and economically using blood test data. ML can serve as an alternative method to rRT-PCR, especially beneficial for resource-constrained countries.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Sport Sciences
Stefano Borghi, Davide Colombo, Antonio La Torre, Giuseppe Banfi, Matteo Bonato, Jacopo Antonino Vitale
Summary: The study found that full backs and wingers consistently had the highest very high-speed running distance and number of sprints in different playing formations, while all positions showed significant decreases in GPS variables in the second half of the match.
RESEARCH IN SPORTS MEDICINE
(2021)
Article
Geriatrics & Gerontology
Marta Carnovali, Giuseppe Banfi, Massimo Mariotti
Summary: The study on male zebrafish scales revealed age-dependent reduction in mineralization rate and increased TRAP activity, with prednisolone promoting bone resorption leading to senile osteoporosis. This new model could potentially help identify early mechanisms of bone aging and develop new therapeutic strategies for age-related bone alterations in humans.
Article
Medical Laboratory Technology
Martina Faraldi, Marta Gomarasca, Silvia Perego, Veronica Sansoni, Giuseppe Banfi, Giovanni Lombardi
Summary: This study investigated the effects of sample matrix, processing, and plasma storage conditions on extracellular vesicles and their miRNA content. Results showed that storage conditions and processing methods can influence the detectability of microRNAs in extracellular vesicles, with K2E samples demonstrating the highest stability.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Biology
Jacopo Antonino Vitale, Giuseppe Banfi, Valentina Tivolesi, Catia Pelosi, Stefano Borghi, Francesco Negrini
Summary: This study used actigraphy to objectively assess physical activity levels and rest-activity daily rhythm in patients undergoing hip or knee joint replacement surgery and found that patients had decreased rest-activity daily rhythm and physical activity levels on the first day after surgery, with gradual improvement from the fourth to the tenth postoperative day. Actigraphy could be a valuable tool in identifying and intervening on patients with disrupted circadian rhythm and reduced physical activity in the peri-operative period of orthopedic surgery.
CHRONOBIOLOGY INTERNATIONAL
(2021)
Article
Materials Science, Multidisciplinary
Federica Buccino, Luigi Zagra, Paolo Savadori, Alessandro Galluzzo, Chiara Colombo, Giacomo Grossi, Giuseppe Banfi, Laura M. Vergani
Summary: The complexity of bone tissue and its hierarchical multi-scale structure make the analysis of bone local mechanical characteristics highly challenging. The distribution of mechanical properties in femoral heads varies between non-osteoporotic and osteoporotic subjects, with changes in load distribution leading to deterioration in trabecular structure patterns.
Article
Health Care Sciences & Services
Federico Pennestri, Giuseppe Banfi
Summary: The Italian Government plans to invest euro15 billion of European funds to digitalize the National Health Service and enhance primary care. Telemedicine is considered a key innovation to achieve these goals, but no details are given on how digitalization and remote care technologies should be implemented. This paper focuses on the contribution of digitalization and telemedicine to primary care innovations, drawing from implemented technology-driven policies. These policies can significantly benefit from digitalization and remote care technology, provided that risks and limitations are considered.
Article
Physiology
Jacopo A. Vitale, Stefano Borghi, Maria Francesca Piacentini, Giuseppe Banfi, Antonio La Torre
Summary: This study investigated the sleep characteristics of elite track-and-field athletes and found significant differences in sleep patterns between sexes and among different disciplines. Female athletes had better sleep quality, while sleep-hygiene strategies had a positive impact on athletes' sleep parameters.
INTERNATIONAL JOURNAL OF SPORTS PHYSIOLOGY AND PERFORMANCE
(2023)