Journal
JOURNAL OF MOLECULAR HISTOLOGY
Volume 40, Issue 3, Pages 227-233Publisher
SPRINGER
DOI: 10.1007/s10735-009-9234-1
Keywords
prelid2; Embryonic development; Unmethylated; In situ hybridization; Real-time quantitative RT-PCR
Categories
Funding
- Ministry of Education Scholarship [GFEQ24403001]
- Harbin Institute of Technology Introduction of Scientific Research Talents Activation Fee [GFQQ18600015]
- Heilongjiang Province Technological Project Program Returning Foundation [LC08C05]
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Prelid2, which belongs to the PRELI domain containing family, is identified as a conserved evolution gene. The expression and regulation during embryonic development of the prelid2 gene is unknown. In this study, we investigated the prelid2 gene expression and regulation using mouse embryos model, by in situ hybridization analysis, RT-PCR and bisulfite sequencing. In situ hybridization analysis showed that prelid2 gene expression were found in midbrain, spinal cord, optic eminence, otic vesicle and tail at E9.5 and E10.5 embryos, in forebrain, hindbrain, heart, lung, liver and kidney at E13.5 and E15.5 embryos. Real-time quantitative RT-PCR results verified the expression pattern in the four major mouse organs, brain, heart, lung, and liver during organs differentiation and formation. Bisulfite sequencing illustrated the consistent result of expression and its unmethylation status in the genomic promoter region at E12.5, E18.5, and new born. Thus, the prelid2 gene is a widely-spread, persistently expressed and unmethylated gene in mouse embryonic development. Our results suggest that the PRELI domain containing 2 gene is involved in mouse embryonic development.
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