Review
Biochemistry & Molecular Biology
Hadla Hariri, Rene St-Arnaud
Summary: This review highlights recent research on the regulatory functions of ubiquitin-specific peptidases (USPs) in osteoblasts and provides insight into the molecular mechanisms governing their actions. It also discusses the role of USP53 in osteoblasts and the complex layers of regulation exerted by USPs on osteoblast signaling. Further studies using knockout mouse models are necessary for a full understanding of the mechanisms underpinning USPs actions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Alessandra Guasto, Valerie Cormier-Daire
Summary: Bone development is regulated by multiple signaling pathways, and their dysregulation can lead to skeletal dysplasia, making it difficult to precisely explain the pathophysiological mechanisms of bone disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Endocrinology & Metabolism
Pauline Raynaud, Camille Gauthier, Vinesh Jugnarain, Frederic Jean-Alphonse, Eric Reiter, Gilles Bruneau, Pascale Crepieux
Summary: Single-domain antibody fragments (VHHs or nanobodies) have shown promising applications in therapeutics and exploration of intracellular processes by reaching cryptic regions in cognate antigens. While most intracellular VHHs/antibodies target cytosolic proteins or transcription factors, there are few that target membrane proteins or G protein-coupled receptors. Understanding their pharmacology has been challenging due to their conformational complexity, but intracellular VHHs have helped to stabilize GPCRs in active conformations for structural study.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Sport Sciences
Ryan N. Marshall, Paul T. Morgan, Benoit Smeuninx, Jonathan I. Quinlan, Matthew S. Brook, Philip J. Atherton, Kenneth Smith, Daniel J. Wilkinson, Leigh Breen
Summary: This study compared the effects of machine resistance training and elastic band resistance training on muscle adaptive remodeling. It found that elastic band resistance training increased myofibrillar protein synthesis in young males, but had limited effects in older males. Further research may be needed to optimize training programs for both age groups.
MEDICINE & SCIENCE IN SPORTS & EXERCISE
(2023)
Article
Physiology
Yue Gao, Nan Chen, Xiangle Zhang, Emma Y. Li, Wei Luo, Jie Zhang, Wenqiang Zhang, Sheng Li, Jian Wang, Suning Liu
Summary: Juvenile hormone (JH) regulates insect development and reproduction through intracellular and membrane signaling. Recent studies have shown that JH membrane signaling induces phosphorylation of Met, enhancing its transcriptional activity. JH also induces phosphorylation of Hsp83, promoting Met nuclear import and transcription. Furthermore, 14-3-3 proteins are negative regulators of Met nuclear import through physical interaction with Hsp83.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell Biology
Bowen Lai, Heng Jiang, Yuan Gao, Xuhui Zhou
Summary: Cilia are conserved structures in eukaryotic cells and their dysfunction can lead to various diseases classified as ciliopathies. Many skeletal phenotypes have been discovered in ciliopathies, including abnormal bone and cartilage development. Mutations in genes encoding cilia core components have been found in skeletal ciliopathies. Signaling pathways associated with cilia and skeleton development are important for these diseases. This review summarizes the structure of cilia, skeletal ciliopathies, and the relevant signaling pathways, with the aim of developing potential therapies.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Carmen Costas-Ferreira, Lilian R. F. Faro
Summary: Pesticides from different chemical classes exert toxic effects on the nervous system by altering calcium homeostasis, leading to oxidative stress, neuroinflammation, and ultimately neuronal death. Proposed strategies to counteract these effects are also discussed in the review.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ariane Zaloszyc, Philippe Choquet, Amira Sayeh, Maria Bartosova, Betti Schaefer, Ulrike Huegel, Gaelle Aubertin-Kirch, Christopher Healy, Francois Severac, Sebastien Rizzo, Georges Boivin, Franz Schaefer, Michel Fischbach, Justine Bacchetta, Seiamak Bahram, Claus Peter Schmitt
Summary: Chronic kidney disease often leads to bone disorders and cardiovascular mortality, and the G alpha(q/11)/PKC pathway in osteoblasts plays an important role in CKD-MBD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Endocrinology & Metabolism
Gongsheng Yuan, Shuying Yang
Summary: This review summarizes the role of RGS proteins in bone homeostasis and diseases, focusing on their regulation of osteoblasts, chondrocytes, and osteoclasts. The impacts of RGS proteins on bone development and related diseases are highlighted, suggesting their potential as drug targets.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Doron Cohn-Schwartz, Yeshai Schary, Eran Yalon, Zoe Krut, Xiaoyu Da, Edward M. Schwarz, Dan Gazit, Gadi Pelled, Zulma Gazit
Summary: The endothelial PTH receptor 1 (PTHR1) signaling plays a key role in PTH-induced osteogenesis and has implications in angiogenesis.
Article
Biochemical Research Methods
Brian D. Young, Jihui Sha, Ajay A. Vashisht, James A. Wohlschlegel
Summary: This study identified novel regulators of G beta gamma signaling in human cells using mass spectrometry, with KCTD2 and KCTD5 shown to specifically recognize G beta gamma and be involved in downstream signal transduction. These KCTD proteins act as substrate adaptors for a multisubunit CUL3-RING ubiquitin ligase, promoting monoubiquitination of G beta(1/2) lysine-23 and regulating downstream signaling. Depletion of these adaptors impairs downstream signaling in cancer cell lines.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biology
Shumin Wang, Eri O. Maruyama, John Martinez, Justin Lopes, Trunee Hsu, Wencheng Wu, Wei Hsu, Takamitsu Maruyama, Ritu Trivedi
Summary: miRNA is an ideal master regulator for organogenesis due to its ability to simultaneously modulate a set of genes for lineage-specific development. However, the lack of obvious phenotypic defects in most miRNA deletions may be attributed to functional redundancy. Our study identifies MIR27a as a specific miRNA essential for bone homeostasis, as its loss causes severe osteoporosis in mice. MIR27a affects bone resorption by osteoclasts and not bone formation by osteoblasts, and its effects are mediated through the regulation of Squstm1/p62.
Review
Cell Biology
Karina A. Pena
Summary: The canonical model for GPCR activation assumes that activation occurs only at the cell surface and is turned off by receptor internalization. However, recent research has shown that some internalized GPCRs can continue to signal from endosomes, which has physiological implications.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Byung-Chul Oh
Summary: Intracellular calcium and phosphoinositides are vital for regulating cellular activities, and their interplay is linked to cancer, metabolic diseases, and insulin resistance. Dysregulation of PIP signaling in cancer cells can lead to membrane localization and activation of PIP-binding proteins. Excessive intracellular calcium levels negatively impact the membrane localization of PIP-binding proteins, contributing to insulin resistance and metabolic diseases.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Biology
Tsz Long Chu, Peikai Chen, Anna Xiaodan Yu, Mingpeng Kong, Zhijia Tan, Kwok Yeung Tsang, Zhongjun Zhou, Kathryn Song Eng Cheah
Summary: This study reveals that MMP14 plays a crucial role in the regulation of PTH signaling, thus contributing to bone homeostasis. During the development of osteoblasts from HC-descendent cells, MMP14 and the PTH pathway are activated. MMP14 cleaves PTH1R to dampen PTH signaling, thereby regulating bone synthesis.
Article
Endocrinology & Metabolism
Maryam Iravani, Marie K. Lagerquist, Elham Karimian, Andrei S. Chagin, Claes OhIsson, Lars Savendahl
ENDOCRINE CONNECTIONS
(2019)
Article
Anatomy & Morphology
Yi Zhang, Polina Kameneva, Karl Annusver, Maria Kasper, Andrei S. Chagin, Igor Adameyko, Meng Xie
DEVELOPMENTAL DYNAMICS
(2020)
Article
Immunology
Yin-Huai Chen, Giedre Grigelioniene, Phillip T. Newton, Jacob Gullander, Maria Elfving, Anna Hammarsjo, Dominyka Batkovskyte, Hessa S. Alsaif, Wesam I. Y. Kurdi, Firdous Abdulwahab, Veerabahu Shanmugasundaram, Luke Devey, Severine Bacrot, Jana Brodszki, Celine Huber, Ben Hamel, David Gisselsson, Nikos Papadogiannakis, Katarina Jedrycha, Barbara Gurtl-Lackner, Andrei S. Chagin, Gen Nishimura, Dominik Aschenbrenner, Fowzan S. Alkuraya, Arian Laurence, Valerie Cormier-Daire, Holm H. Uhlig
JOURNAL OF EXPERIMENTAL MEDICINE
(2020)
Review
Pediatrics
Andrei S. Chagin, Phillip T. Newton
PEDIATRIC RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
M. Peleli, D. M. S. Ferreira, L. Tarnawski, S. McCann Haworth, L. Xuechen, Z. Zhuge, P. T. Newton, J. Massart, A. S. Chagin, P. S. Olofsson, J. L. Ruas, E. Weitzberg, J. O. Lundberg, M. Carlstrom
Article
Cell Biology
Yi Zhang, Karl Annusver, Kazunori Sunadome, Polina Kameneva, Steven Edwards, Guanghua Lei, Maria Kasper, Andrei S. Chagin, Igor Adameyko, Meng Xie
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Review
Cell Biology
Anastasiia D. Kurenkova, Ekaterina V. Medvedeva, Phillip T. Newton, Andrei S. Chagin
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Endocrinology & Metabolism
Meng Xie, Andrei S. Chagin
Summary: Bone age is a widely used method by pediatricians to assess skeletal maturity and predict growth potential in children. It involves measuring the size of secondary ossification centers that develop in the ends of long bones as a child ages. However, little is currently known about the developmental biology, evolution, and functional role of these skeletal elements.
Article
Biology
Meng Xie, Pavel Gol'din, Anna Nele Herdina, Jordi Estefa, Ekaterina V. Medvedeva, Lei Li, Phillip T. Newton, Svetlana Kotova, Boris Shavkuta, Aditya Saxena, Lauren T. Shumate, Brian D. Metscher, Karl Grossschmidt, Shigeki Nishimori, Anastasia Akovantseva, Anna P. Usanova, Anastasiia D. Kurenkova, Anoop Kumar, Irene Linares Arregui, Paul Tafforeau, Kaj Fried, Mattias Carlstrom, Andras Simon, Christian Gasser, Henry M. Kronenberg, Murat Bastepe, Kimberly L. Cooper, Peter Timashev, Sophie Sanchez, Igor Adameyko, Anders Eriksson, Andrei S. Chagin
Article
Biochemistry & Molecular Biology
Shabnam I. Ibragimova, Ekaterina V. Medvedeva, Irina A. Romanova, Leonid P. Istranov, Elena V. Istranova, Aleksey V. Lychagin, Andrey A. Nedorubov, Peter S. Timashev, Vladimir I. Telpukhov, Andrei S. Chagin
Summary: Articular cartilage repair remains a challenging task and tissue engineering has been widely employed in joint tissue reconstruction. However, the optimal scaffolds for cartilage repair are still unknown. This study compared five different scaffolds and found that decellularized cartilage and collagen type II-based scaffold almost fully restored the congruence of the articular surface. However, the formation of hyaline cartilage was not observed in any group. It was concluded that for restoration of hyaline cartilage, scaffolds need to be combined with cellular therapy or morphogens promoting chondrogenic differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Marketa Kaucka, Alberto Joven Araus, Marketa Tesarova, Joshua D. Currie, Johan Bostrom, Michaela Kavkova, Julian Petersen, Zeyu Yao, Anass Bouchnita, Andreas Hellander, Tomas Zikmund, Ahmed Elewa, Phillip T. Newton, Ji-Feng Fei, Andrei S. Chagin, Kaj Fried, Elly M. Tanaka, Jozef Kaiser, Andras Simon, Igor Adameyko
Summary: The study analyzes limb development and regeneration in amphibians, and reveals that regeneration is based on cartilage expansion and ossification follows after the final size of regenerating cartilage is reached, leading to bulkier bones. The process of ossification starts from the cortical bone and proceeds inwards in both regenerative and developmental processes, showing the diversity of schemes for the synchrony of cortical and endochondral ossification among vertebrates.
NATURE COMMUNICATIONS
(2022)
Article
Endocrinology & Metabolism
Dominyka Batkovskyte, Fiona McKenzie, Fulya Taylan, Pelin Ozlem Simsek-Kiper, Sarah M. Nikkel, Hirofumi Ohashi, Roger E. Stevenson, Thuong Ha, Denise P. Cavalcanti, Hiroyuki Miyahara, Steven A. Skinner, Miguel A. Aguirre, Zuehal Akcoeren, Gulen Eda Utine, Tillie Chiu, Kenji Shimizu, Anna Hammarsjoe, Koray Boduroglu, Hannah W. Moore, Raymond J. Louie, Peer Arts, Allie N. Merrihew, Milena Babic, Matilda R. Jackson, Nikos Papadogiannakis, Anna Lindstrand, Ann Nordgren, Christopher P. Barnett, Hamish S. Scott, Andrei S. Chagin, Gen Nishimura, Giedre Grigelioniene
Summary: Lethal short-limb skeletal dysplasia Al-Gazali type is a rare disorder with unknown genetic etiology. A study involving nine patients with clinical features consistent with this disorder identified disease-causing variants in ADAMTSL2, shedding light on the genetic cause and highlighting the importance of analyzing the pseudogene region. This study enhances our understanding of Al-Gazali skeletal dysplasia and its association with ADAMTSL2-related disorders.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Anastasiia D. Kurenkova, Lei Li, Anna P. Usanova, Xiaogang Feng, Baoyi Zhou, Andrei A. Nedorubov, Alexey Lychagin, Andrei S. Chagin
Summary: Articular cartilage has limited ability to self-repair, and clinical approaches to cartilage regeneration are necessary. The current method involves expansion of autologous chondrocytes followed by reimplantation, but the outcome is often unsatisfactory due to dedifferentiation. In recent years, articular stem/progenitor cells (artSPCs) have been discovered, which have the potential to repair damaged cartilage. This study found that modulation of the Notch pathway during expansion facilitated chondrogenic potential of artSPCs and chondrocytes, while activation of the pathway abrogated this potential.
Meeting Abstract
Endocrinology & Metabolism
Baoyi Zhou, Ameya Bendre, Lars Savendahl, Ola Nilsson, Andrei Chagin
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Dominyka Batkovskyte, Fiona McKenzie, Fulya Taylan, Pelin Ozlem Simsek-Kiper, Sarah M. Nikkel, Hirofumi Ohashi, Hiroyuki Miyahara, Gustaw Eriksson, Thuong Ha, Gulen Eda Utine, Tillie Chiu, Kenji Shimizu, Anna Hammarsjo, Koray Boduroglu, Peer Arts, Milena Babic, Matilda R. Jackson, Nikos Papadogiannakis, Anna Lindstrand, Ann Nordgren, Christopher P. Barnett, Hamish S. Scott, Andrei S. Chagin, Gen Nishimura, Giedre Grigelioniene
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
