Article
Chemistry, Analytical
Jun-Hao Wan, Chen Qian, Gang Wu, Xian-Wei Liu
Summary: Label-free imaging of nanoscale targets with intrinsic properties is crucial for unveiling the underlying mechanisms of chemistry, physics, and life science. Plasmonic imaging techniques provide real-time imaging and insights into nanoscale detection and nanocatalysis. In this study, a high-resolution plasmonic imaging method is presented, demonstrating its capability to image various nanomaterials and accurately track the interfacial dynamics of nanoparticles. With the simplicity and capacity for label-free and real-time imaging, this approach serves as a promising platform for characterizing nanomaterials at the single-particle level.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
M. R. Vishnuraj, G. Ajay, N. Aravind Kumar, J. Renuka, Niveditha Pollumahanti, H. Anusha Chauhan, S. Vaithiyanathan, Deepak B. Rawool, S. B. Barbuddhe
Summary: A duplex, SYBR green-based, real-time PCR assay was developed for detection and differentiation of Listeria and Listeria monocytogenes. The assay showed high sensitivity and accuracy, and was validated using standard parameters and international standards. It was successfully applied to artificially spiked and surveillance samples, making it a reliable tool for addressing Listeria contamination in food safety.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Biochemistry & Molecular Biology
Camille Kessler, Alice Brambilla, Dominique Waldvogel, Glauco Camenisch, Iris Biebach, Deborah M. Leigh, Christine Grossen, Daniel Croll
Summary: The study explores the impact of immune function polymorphism on health and reproductive success within species, as well as the significance of genetic diversity loss in extinction risk. Through the design of a microfluidics-based amplicon sequencing assay, comprehensive genetic variation in Alpine ibex was successfully captured, revealing unexpectedly high genetic differentiation at immunity-related loci within the species.
MOLECULAR ECOLOGY RESOURCES
(2022)
Article
Biochemistry & Molecular Biology
Priyanka Reddy, Aaron Elkins, Joe Panozzo, Simone J. Rochfort
Summary: A fast LC-MS method for determining amino acids has been developed, requiring only three simple steps of hydrolysis, neutralisation, and sample dilution with a borate buffer solution. The method shows excellent repeatability and reproducibility for amino acid content, peak area, and retention time. Detection levels on column are below 0.1 μM for most amino acids.
Article
Microbiology
S. A. Higgins, K. Panke-Buisse, Daniel H. Buckley
Summary: The study revealed that the diversity of Streptomyces is correlated with soil pH, mean annual temperature, and plant community richness, but not with latitude. Soil pH and plant community richness explain significant variations in Streptomyces beta diversity, with high dissimilarity and strong dominance of a few species observed at each site. These results indicate that dispersal limitation due to competitive interactions constrains the colonization success of spores in new sites.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Optics
Xin Zhang, Ke Kang, Yanfang Jiang, Jiaxue He, Yanfeng Qiao
Summary: This study proposes a novel double-layer focal plane microscope that doubles the throughput of DNA sequencing. By dividing the fluorescence channels and adjusting the camera, the information capacity of the microscope is effectively increased. Experimental results show that the throughput is doubled while the signal-to-noise ratio of the images is slightly reduced.
Article
Chemistry, Analytical
Shuaizhen Zhou, Zia Fatma, Pu Xue, Shekhar Mishra, Mingfeng Cao, Huimin Zhao, Jonathan Sweedler
Summary: To meet the increasing need for high-throughput screening in metabolic engineering, a fast and information-rich screening method is required. Liquid extraction surface analysis coupled with mass spectrometry (LESA-MS) is an efficient approach that directly samples and introduces metabolites from a surface to MS. In this study, LESA-MS combined with an acoustic liquid handler and stable isotope-labeled internal standards, enabling absolute quantitation of target chemicals and high-throughput screening for microbial metabolites.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Jianshi Jin, Reiko Yamamoto, Tadashi Takeuchi, Guangwei Cui, Eiji Miyauchi, Nozomi Hojo, Koichi Ikuta, Hiroshi Ohno, Katsuyuki Shiroguchi
Summary: The study develops a method called Barcoding Bacteria for Identification and Quantification (BarBIQ) that allows for the classification and quantification of individual bacterial cells in the microbiota. It is found that BarBIQ reveals location-dependent differences in the microbiota, which cannot be shown by conventional 16S rRNA gene-amplicon sequencing methods.
NATURE COMMUNICATIONS
(2022)
Article
Optics
Weisong Zhao, Shiqun Zhao, Zhenqian Han, Xiangyan Ding, Guangwei Hu, Liying Qu, Yuanyuan Huang, Xinwei Wang, Heng Mao, Yaming Jiu, Ying Hu, Jiubin Tan, Xumin Ding, Liangyi Chen, Changliang Guo, Haoyu Li
Summary: High-throughput super-resolution (SR) imaging is attractive for rapid and high-precision profiling in biomedical applications. The proposed SACD method reduces the number of frames required for SR imaging and enables four-dimensional imaging of live cells and events. SACD improves accessibility to SR imaging and facilitates high-throughput and low-cost biological studies.
Article
Biochemical Research Methods
Olga Bazalova, Jaromir Z. Cihlar, Zuzana Dlouha, Ladislav Bar, Vladimir Drab, Miloslava Kavkova
Summary: This study focused on the rapid identification of sourdough yeasts, utilizing a panfungal real time-PCR targeting the ITS2 region and a fragment of D1/D2 region of 26S rRNA gene, followed by high resolution melting analysis for species identification. The method showed high sensitivity and specificity in testing yeasts in reference cultures and sourdough samples.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Article
Chemistry, Applied
Yan Xu, Haixia Li, Jian Liang, Jina Ma, Juan Yang, Xiaojuan Zhao, Wenhong Zhao, Weidong Bai, Xiaofang Zeng, Hao Dong
Summary: A novel method based on HPLC-Q-Orbitrap-HRMS was developed for simultaneous determination of eighteen HAAs, which was successfully validated and applied for HAAs analysis in roasted and pan-fried meat.
Article
Automation & Control Systems
Chunxiang Wang, Mingsi Tong, Liqun Zhao, Xinghu Yu, Songlin Zhuang, Huijun Gao
Summary: The study introduces DanioSense (DS), an automatic tracker for group larval zebrafish, that overcomes challenges such as similar appearances, frequent occlusions, and highly discontinuous kinematics through the integration of a light convolutional neural network and a centerline extraction algorithm. DS is capable of localizing individuals even in occlusion cases where object identities are prone to switch, providing detailed quantitative data for a large-scale larvae group in nearly real time.
IEEE TRANSACTIONS ON AUTOMATION SCIENCE AND ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Jibin Abraham Punnoose, Kevin J. Thomas, Arun Richard Chandrasekaran, Javier Vilcapoma, Andrew Hayden, Kacey Kilpatrick, Sweta Vangaveti, Alan Chen, Thomas Banco, Ken Halvorsen
Summary: Stacking interactions between adjacent bases in DNA and RNA are important for biological processes and biotechnology applications. In this study, the authors used a centrifuge force microscope to measure stacking energies between individual bases. They found that stacking energies are strongest between purines (G|A) and weakest between pyrimidines (C|T). Phosphorylated, methylated, and RNA nucleotides had no effect on stacking energies, but a fluorophore modification reduced stacking energy.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Fang Xu, Yan Shen, Lufeng Ding, Chao-Yu Yang, Heng Tan, Hao Wang, Qingyuan Zhu, Rui Xu, Fengyi Wu, Yanyang Xiao, Cheng Xu, Qianwei Li, Peng Su, Li I. Zhang, Hong-Wei Dong, Robert Desimone, Fuqiang Xu, Xintian Hu, Pak-Ming Lau, Guo-Qiang Bi
Summary: This study introduces a new method combining primate-optimized tissue sectioning and clearing with high-speed fluorescence microscopy to achieve high-resolution imaging of the entire rhesus monkey brain. Researchers have also developed an efficient method for tracing sparse axonal fibers in large datasets, enabling connectome-scale mapping of large primate brains.
NATURE BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ting Wu, Danli Jiang, Meijuan Zou, Wei Sun, Di Wu, Jing Cui, Ian Huntress, Xinxia Peng, Gang Li
Summary: This study utilized Reel-seq and FREP-MS analysis techniques to systematically identify cis-regulatory elements (cis-REs) at high resolution in the human genome, revealing their role in regulating gene expression by recruiting specific proteins and transcription factors, thereby impacting cellular senescence.
NUCLEIC ACIDS RESEARCH
(2022)