Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 59, Issue 4, Pages 297-301Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2008.11.013
Keywords
beta-Glucosidase; Isoflavone; Aglycone; Aspergillus oryzae; Superoxide dismutase (sodM) promoter
Funding
- Research and Development Program for New Bio-industry Initiatives
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beta-Glucosidase (BGL1) from Aspergillus oryzae was efficiently produced in recombinant A. oryzae using sodM promoter-mediated expression system. The yield of BGL1 was 960 mg/l in liquid culture, which is 20-fold higher than the yield of BGL1 produced using the yeast Saccharomyces cerevisiae. Recombinant BGL1 converted isoflavone glycosides into isoflavone aglycones more efficiently than P-glucosidase from almond. In addition, BGL1 produced isoflavone aglycones even in the presence of the insoluble form of isoflavone glycosides. (C) 2008 Elsevier B.V. All rights reserved.
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