Article
Environmental Sciences
Christian M. Bako, Timothy E. Mattes, Rachel F. Marek, Keri C. Hornbuckle, Jerald L. Schnoor
Summary: The experiments showed that the aerobic PCB-degrading bacteria Paraburkholderia xenovorans LB400 can rapidly degrade most PCB congeners when they are freely dissolved in liquid, but cannot degrade PCB congeners with three or more chlorine substituents in sediment slurry. Using GC/MS-MS triple quadrupole spectrometry, the study distinguished between physical (sorption to cells) and biological removal mechanisms, and illuminated how microorganisms like LB400 can selectively transform lower-chlorinated congeners over time.
ENVIRONMENTAL POLLUTION
(2021)
Article
Engineering, Environmental
Christian M. Bako, Andres Martinez, Jessica M. Ewald, Jason B. X. Hua, David J. Ramotowski, Qin Dong, Jerald L. Schnoor, Timothy E. Mattes
Summary: In this study, we found that bioaugmentation with aerobic, polychlorinated biphenyl (PCB) degrading microorganisms can effectively reduce PCB emissions from contaminated sediment to air. Through synthetic samples and qPCR analysis, it was observed that the total PCB mass in the bioaugmented treatment decreased by 57% on average, with a preferential biodegradation of lower-chlorinated PCBs. The use of the phytogenic biosurfactant, saponin, was also found to enhance the abundance of the degrading microorganisms and promote the biodegradation process. These findings demonstrate that bioaugmentation can be an effective approach to reduce PCB emissions and minimize exposure risks in surrounding communities.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Physical
Elif Erdem, Lenny Malihan-Yap, Leen Assil-Companioni, Hanna Grimm, Giovanni Davide Barone, Carole Serveau-Avesque, Agnes Amouric, Katia Duquesne, Veronique de Berardinis, Yagut Allahverdiyeva, Veronique Alphand, Robert Kourist
Summary: BVMOXeno is a highly efficient Baeyer-Villiger monooxygenase with higher reaction rates and lower KM value compared to currently known BVMOs, almost completely suppressing unwanted side reactions. The recombinant production of BVMO in cyanobacteria allows more efficient utilization of water and oxygen generated by photosynthesis.
Article
Immunology
Melanie Gonzales, Laure Plener, Jean Armengaud, Nicholas Armstrong, Eric Chabriere, David Daude
Summary: This study demonstrates the importance of Quorum sensing in Burkholderia thailandensis using an enzyme-based quorum quenching strategy. The disruption of quorum sensing greatly affects bacterial behavior, including motility, proteolytic activity, and antimicrobial molecule production. Furthermore, the study shows that quorum sensing disruption significantly enhances the bactericidal activity against fungi and yeast. These findings are of prime interest for understanding the virulence of Burkholderia species and developing alternative treatments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Christian M. Bako, Timothy E. Mattes, Rachel F. Marek, Keri C. Hornbuckle, Jerald L. Schnoor
Summary: The dataset details the biodegradation of PCB congeners by Paraburkholderia xenovorans LB400 in different conditions. The extraction methods, sample processing, and correction procedures ensure the accuracy and reproducibility of the data, which can inform future experimental designs.
Review
Biotechnology & Applied Microbiology
Yuanyuan Fang, Zhenxia Zhang, Wei Xu, Wenli Zhang, Cuie Guang, Wanmeng Mu
Summary: This review comprehensively presents the gene mining, characterization, molecular modification, and application of microbial-derived ZEN lactonases, aiming to elucidate the advantages and challenges of ZEN lactonases in industrial application, which also provides perspectives on obtaining innovative and promising biocatalysts for ZEN degradation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Yuanyuan Fang, Zhaolin Huang, Wei Xu, Chengfei Wang, Yurong Sun, Wenli Zhang, Cuie Guang, Wanmeng Mu
Summary: In this study, two potential hotspots were identified in the zearalenone lactonase from Gliocladium roseum. Mutants with increased thermostability were obtained and showed improved performance in high temperature conditions for efficient elimination of zearalenone.
Article
Chemistry, Physical
Xiaowu Fang, Songwei Wen, Peishen Jin, Wenjing Bao, Shanshan Liu, Hengjiang Cong, Xiao Shen
Summary: Fluorinated enol silanes are widely used in synthetic transformations, but previous methods only allowed for the synthesis of racemic products. In this study, an enantioselective reductive coupling reaction was developed to access enantioenriched fluorinated enol silanes. The practicality of this methodology was demonstrated through multistep-one-isolation processes, and the synthetic potential of enantioenriched fluorinated enol silanes in the synthesis of valuable chiral organofluorine compounds was also shown.
Article
Oncology
Marius Raica, Amalia Raluca Ceausu, Anca Maria Cimpean, Serban Comsa, Simona Sarb
Summary: The study investigated the expression of CLIC1, Ecad, and Pcad in breast cancer, revealing their association with molecular subtypes and the ability to define new high-risk subgroups. This stratification may explain variations in treatment response and aggressiveness observed by different authors within the same molecular subtype.
ANTICANCER RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Evelise Bach, Fernando Hayashi Sant'Anna, Guilherme Dubal dos Santos Seger, Luciane Maria Pereira Passaglia
Summary: This study performed pangenome analysis on Burkholderia sensu lato for the first time, and focused on the specific features of B. catarinensis in the pangenome of Burkholderia sensu stricto and Burkholderia cepacia complex (Bcc). The results showed great variability and unknown potential in these genomes. The genome of B. catarinensis contains genes related to hydmlase production and plant growth promotion.
Article
Agriculture, Multidisciplinary
Binbin Ouyang, Wenli Zhang, Cuie Guang, Wei Xu, Wanmeng Mu
Summary: This study characterized a thermostable ZEN lactonase (ZENM) from Monosporascus sp. GIB2, which exhibited high activity at 60 degree celsius and efficient hydrolysis of ZEN and its derivatives. The mutant G163S in the cap domain of ZENM demonstrated enhanced activity towards alpha-ZOL, suggesting a modified substrate specificity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Organic
Kevin Ng, Michael Khoury, Thomas Gerard Minehan
Summary: Total syntheses of sesquiterpenes (+)-sootepdienone, (-)-jambolanin C, (-)-jambolanin I, and (-)-gibberodione have been achieved in 10 steps each from R-(+)-pulegone, allowing determination of the absolute configuration of the natural products. A key step in the synthetic pathways involves the one-carbon ring expansion of a cyclic allylic phosphonate to a substituted cycloheptenone through a tandem oxidative cleavage/intramolecular Horner-Wadsworth-Emmons reaction.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Lili Pan, Chao Hong, Lai N. Chan, Gang Xiao, Parmanand Malvi, Mark E. Robinson, Huimin Geng, Srinivasa T. Reddy, Jaewoong Lee, Vishal Khairnar, Kadriye Nehir Cosgun, Liang Xu, Kohei Kume, Teresa Sadras, Shaoyuan Wang, Narendra Wajapeyee, Markus Muschen
Summary: B cell acute lymphoblastic leukemia (B-ALL) patients show aberrant expression of the lactonase PON2, bypassing metabolic gatekeeper functions. Deficiency of PON2 compromises glucose uptake and ATP production in B-ALL cells, while its lactonase activity can be used as synthetic lethality to overcome drug resistance in refractory B-ALL.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Microbiology
Ella M. Meumann, Direk Limmathurotsakul, Susanna J. Dunachie, Willem J. Wiersinga, Bart J. Currie
Summary: This review explores the global burden, epidemiology, and pathophysiology of Burkholderia pseudomallei, the causative agent of melioidosis, as well as current diagnostics, treatment recommendations, and preventive measures. Melioidosis is widely distributed in tropical and subtropical regions and has been proposed as a neglected tropical disease by the World Health Organization. Climate change and environmental disturbance are expected to increase the number of cases.
NATURE REVIEWS MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yun Heacock-Kang, Ian A. McMillan, Michael H. Norris, Zhenxin Sun, Jan Zarzycki-Siek, Andrew P. Bluhm, Darlene Cabanas, Robert E. Norton, Natkunam Ketheesan, Jeff F. Miller, Herbert P. Schweizer, Tung T. Hoang
Summary: Prokaryotic cell transcriptomics has been limited to mixed or sub-population dynamics and individual cells within heterogeneous populations. The authors develop a 'TRANSITomic' approach to profile transcriptomes of single Burkholderia pseudomallei cells as they transit through host cell infection, revealing dynamic gene-expression flux during transit in host cells and identifying genes that are required for pathogenesis. The 'TRANSITome' approach enables high-resolution understanding of host-pathogen interactions.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)