Review
Biochemistry & Molecular Biology
Manuel Halte, Marc Erhardt
Summary: The T3SS utilizes proton motive force to export substrate proteins across the inner membrane of pathogenic bacteria. It consists of an export gate and a soluble ATPase complex in the cytoplasm. Recent research on T3SS focuses on energy transduction and protein transport across the cytoplasmic membrane.
Review
Microbiology
Liam J. Worrall, Dorothy D. Majewski, Natalie C. J. Strynadka
Summary: This article describes two fascinating bacterial nanomachines - the widely distributed rotary flagellum and the eukaryotic cell-puncturing injectisome. Both nanomachines utilize the type III secretion system (T3SS) to secrete components and translocate proteins. Advances in structural and functional understanding, facilitated by cryoelectron microscopy technologies, have provided insights into these nanomachines since 2015.
ANNUAL REVIEW OF MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lu-Lu He, Xin Wang, Dylan O'Neill Rothenberg, Xiaoli Xu, Hai-Hong Wang, Xin Deng, Zi-Ning Cui
Summary: In this study, a high-throughput screening reporter system was constructed to screen for imidazole, oxazole, and thiazole compounds. The screening identified three compounds that significantly inhibited the activity of hrpW and hrpL gene promoters in P. syringae without affecting its growth. These compounds were found to suppress the expression of T3SS genes and inhibit the pathogenicity of P. syringae in tomato and bean. The findings suggest the potential of novel P. syringae T3SS inhibitors for disease prevention and control.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Julie Ming Liang, Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: Protein production using bacteria is costly and inefficient. Researchers have engineered a bacterial secretion system to improve the production process by directly secreting soluble proteins into the culture media. They explored different activation methods and created strains capable of efficiently producing heterologous proteins. They also discovered a genetic relationship between T3SS regulators, hilE and hilD, further enhancing the industrial competitiveness of protein production strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Stephan Wimmi, Alexander Balinovic, Hannah Jeckel, Lisa Selinger, Dimitrios Lampaki, Emma Eisemann, Ina Meuskens, Dirk Linke, Knut Drescher, Ulrike Endesfelder, Andreas Diepold
Summary: Current research shows that in gastrointestinal pathogens, the external pH plays a crucial role in regulating the assembly of T3SS cytosolic components, preventing T3SS activity in the stomach and facilitating its reactivation in the intestine.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Marc-Andre Leblanc, Morgan R. Fink, Thomas T. Perkins, Marcelo C. Sousa
Summary: The study shows that effector proteins from certain bacteria can be injected into host cells through a type III secretion system. These proteins need to be partially unfolded to pass through the narrow channel. Mechanical unfolding of the proteins revealed that they exhibit low force and high mechanical compliance, facilitating efficient secretion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Julia Sanchez-Garrido, David Ruano-Gallego, Jyoti S. Choudhary, Gad Frankel
Summary: Recent studies have shown that T3SS effectors interact with each other in the host, revealing their interdependency and context-dependent essentiality, which is of great significance for understanding the pathogenesis of the pathogens.
TRENDS IN MICROBIOLOGY
(2022)
Article
Microbiology
Kevin Munoz Navarrete, Ladislav Bumba, Tatyana Prudnikova, Ivana Malcova, Tania Romero Allsop, Peter Sebo, Jana Kamanova
Summary: The study reveals that BopN is a gatekeeper of the Bordetella T3SS, responsible for regulating the secretion and translocation of the BteA effector into host cells. It is similar to gatekeepers that control access to the T3SS channel from the bacterial cytoplasm. Deletion of the bopN gene in B. bronchiseptica increased secretion of BteA, but decreased the translocation of BteA into host cell cytoplasm.
MICROBIOLOGY SPECTRUM
(2023)
Review
Microbiology
Elizabeth A. Rucks
Summary: Type III secretion systems (T3SS) are utilized by Gram-negative pathogens to deliver effector proteins into target eukaryotic cells for manipulating cell functions and enhancing pathogenesis. This review will discuss the history, biochemical characterization, and function of chlamydial T3SS, as well as the use of heterologous/surrogate models for studying it. The review will also cover the history of chlamydial effectors and recent advances in the field.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Microbiology
Anqun Hu, Ming Hu, Shanshan Chen, Yang Xue, Xu Tan, Jianuan Zhou
Summary: The study identified five compounds that can effectively inhibit soft rot symptoms caused by Dickeya zeae, reduce hypersensitive response on non-host plants, and downregulate the expression of T3SS, providing potential biocontrol agents for the prevention of soft rot disease.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Gastroenterology & Hepatology
Ce Zhang, Min Liu, Ying Wu, Xixi Li, Chen Zhang, Douglas R. Call, Ming Liu, Zhe Zhao
Summary: In Vibrio parahaemolyticus, the expression of T3SS1 is regulated by a complex quorum sensing phosphorelay circuit involving the hybrid histidine kinase ArcB, sensor kinase LuxQ, and response regulator LuxO. The study shows that LuxQ can directly interact with ArcB under high oxygen conditions, leading to phosphorylation of LuxO and activation of T3SS1 expression. Under hypoxic conditions, ArcB autophosphorylates and phosphorylates ArcA, but ArcA does not participate in regulating T3SS1 expression.
Article
Biochemistry & Molecular Biology
Bailey Milne-Davies, Stephan Wimmi, Andreas Diepold
Summary: The type III secretion system is the common core of two bacterial molecular machines: the flagellum and the injectisome. It is characterized by flexibility, adaptivity, and a dynamic nature.
MOLECULAR MICROBIOLOGY
(2021)
Review
Immunology
Albane Jouault, Alessandra Mattos Saliba, Lhousseine Touqui
Summary: This mini-review discusses how the type III secretion system (T3SS) of Pseudomonas aeruginosa and the bacterial effectors secreted through this system can activate and manipulate the host immune response, promoting the survival and establishment of the pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nidhi Singh, Thales Kronenberger, Andrea Eipper, Felix Weichel, Mirita Franz-Wachtel, Boris Macek, Samuel Wagner
Summary: The study found that the salt bridges in the assembly of virulence-associated T3SS are not critical, but facilitate the incorporation of the next subunit. The conserved charged residues are crucial for type III-dependent secretion and play a key role in the function of T3SS.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Electrochemistry
Mikako Saito, Shoya Hiratoko, Ikuko Fukuba, Shin-ichi Tate, Hideaki Matsuoka
Article
Microbiology
Tomoharu Inoue, Clive S. Barker, Hideyuki Matsunami, Shin-Ichi Aizawa, Fadel A. Samatey
Article
Biochemistry & Molecular Biology
Kohei Umehara, Miho Hoshikawa, Naoya Tochio, Shin-ichi Tate
Article
Biophysics
Alexandra Born, Parker J. Nichols, Morkos A. Henen, Celestine N. Chi, Dean Strotz, Peter Bayer, Shin-Ichi Tate, Jeffrey W. Peng, Beat Vogeli
BIOMOLECULAR NMR ASSIGNMENTS
(2019)
Article
Biochemistry & Molecular Biology
Hiroaki Ito, Takeshi Sugawara, Soya Shinkai, Satoshi Mizukawa, Ayaka Kondo, Hisamichi Senda, Kengo Sawai, Koki Ito, Sayaka Suzuki, Masakatsu Takaine, Satoshi Yoshida, Hiromi Imamura, Kenji Kitamura, Toshinori Namba, Shin-ichi Tate, Masaru Ueno
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Meeting Abstract
Biophysics
Shin-ichi Tate
BIOPHYSICAL JOURNAL
(2019)
Article
Biochemistry & Molecular Biology
Vladimir A. Meshcheryakov, Satoshi Shibata, Makoto Tokoro Schreiber, Alejandro Villar-Briones, Kenneth F. Jarrell, Shin-Ichi Aizawa, Matthias Wolf
Article
Microbiology
Tomohiro Kimura, Takeaki Tezuka, Daisuke Nakane, Takayuki Nishizaka, Shin-Ichi Aizawa, Yasuo Ohnishi
JOURNAL OF BACTERIOLOGY
(2019)
Article
Pharmacology & Pharmacy
Tetsuya Ohta, Risato Yamada, Satoshi Fujita, Taketoshi Takahata, Kohei Shiba, Sachiko Machida, Shin-ichi Tate
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Satoshi Shibata, Hideyuki Matsunami, Shin-Ichi Aizawa, Matthias Wolf
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Miki Kinoshita, Seina Tanaka, Yumi Inoue, Keiichi Namba, Shin-Ichi Aizawa, Tohru Minamino
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Ryosuke Kawasaki, Shin-ichi Tate
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Microbiology
Yuzuki Shimamori, Ajeng K. Pramono, Tomoe Kitao, Tohru Suzuki, Shin-ichi Aizawa, Tomoko Kubori, Hiroki Nagai, Shigeki Takeda, Hiroki Ando
Summary: A novel bacteriophage SaGU1 was characterized in this study from sewage samples in Japan, showing potential as a phage therapy agent against S. aureus infections. SaGU1 demonstrated heat stability, lytic activity, and a broad host range targeting clinical isolates of S. aureus on the skin of AD patients. It is considered a promising candidate for developing phage therapy to treat AD caused by pathogenic S. aureus.
CURRENT MICROBIOLOGY
(2021)
Article
Microbiology
Yuki Yamanaka, Shin-Ichi Aizawa, Kaneyoshi Yamamoto
Summary: Escherichia coli's acid resistance system, known as the GAD system, is linked to its flagellar system. The HdeD protein in the GAD system regulates flagellum synthesis and affects the motility and survival efficiency of the bacteria.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kyota Yasuda, Tomonobu M. Watanabe, Myeong-Gyun Kang, Jeong Kon Seo, Hyun-Woo Rhee, Shin-ichi Tate
Summary: The presence of valosin-containing protein (VCP) in FUS granules plays a role in regulating their stability, with VCP consuming ATP to reduce ATP concentrations in the granules. Initial colocalization of VCP stabilizes the granules, while prolonged colocalization destabilizes the granules.
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)