Article
Microbiology
Mikhail I. Dobrikov, Elena Y. Dobrikova, Zachary P. McKay, Jonathan P. Kastan, Michael C. Brown, Matthias Gromeier
Summary: MDA5, among the RIG-I-like pattern recognition receptors, can sense long dsRNA duplexes independent of their 5' features. Our study demonstrates that prior to engaging MDA5, recombinant PV RNA is sensed upon PKR binding to the viral IRES, which interferes with viral protein synthesis.
Editorial Material
Microbiology
David Aponte-Diaz, Matthew R. Vogt, Craig E. Cameron
Summary: EV-D68 contributes significantly to respiratory and neurological diseases. It exploits cellular autophagic compartments and its viral proteins 2B and 3A have difficulty engaging membranes, leading to a delay in RNA synthesis.
Article
Biochemistry & Molecular Biology
Kangkang Niu, Xiaojuan Zhang, Qisheng Song, Qili Feng
Summary: This study demonstrates that RBM4 promotes mRNA translation of the VEGFA gene by binding to the G4 structure in the IRES.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Nana Wang, Haiwei Wang, Jiabao Shi, Chen Li, Xinran Liu, Junhao Fan, Chao Sun, Craig E. Cameron, Hong Qi, Li Yu
Summary: Senecavirus A (SVA) is a picornavirus that causes vesicular disease in swine and is the only member of its genus. The IRES in SVA plays a crucial role in cap-independent translation. Studies have shown that the stem-loop I element in SVA's IRES is essential for IRES activity and viral replication.
Review
Biochemistry & Molecular Biology
Dmitry E. Andreev, Michael Niepmann, Ivan N. Shatsky
Summary: The translation phenomenon of internal initiation was discovered on poliovirus mRNA in 1988. This article discusses the poorly characterized type I IRES elements and provides evidence for the major regulators, Poly(rC) binding protein 2 (PCBP2), Glycyl-tRNA synthetase (GARS), and Cold Shock Domain Containing E1 (CSDE1), of type I IRES activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Jessika Poliana Teixeira, Teodorico Castro Ramalho
Summary: Autism Spectrum Disorder (ASD) is a disorder characterized by changes in social and cognitive skills, but the etiology is complex and therapeutic progress is limited. Dysfunctions in synaptic protein synthesis observed in patients with ASD may be targeted through research on the mTOR pathway for potential new treatments.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Jack D. Godfrey, Daniel Hejazi, Xiaofei Du, Cenfu Wei, Eshaan Rao, Christopher M. Gomez
Summary: The HER2/neu signaling pathway is frequently mutated in human cancer and targeting this pathway is effective in treatment. However, cancer cells often develop resistance. This study shows that smaller c-terminal fragments (CTFs) of the HER2 protein, generated through internal translation, are a cause of resistance. Understanding this mechanism could lead to new therapeutic interventions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Tingyu Peng, Fayu Yang, Fan Yang, Weijun Cao, Haixue Zheng, Zixiang Zhu
Summary: The genomic arrangement of most picornavirus of the Picornaviridae family shares a similar monocistronic genomic pattern and a defining organizational feature, which includes evolutionarily conserved and highly-structured RNA elements in the untranslated regions (UTRs). However, the functional characterization and biological significance of UTRs, especially the 5' UTR, are still not fully understood due to the diversity and complexity of RNA structure and limitations of molecular biology techniques. This review summarizes the current knowledge of the 5' UTR of picornavirus, focusing on its structural characterization and biological function of RNA secondary and tertiary structures. Understanding the role of the 5' UTR of picornavirus provides insights into viral replication cycle and pathogenic mechanisms.
Article
Neurosciences
Valeria Ruggiero, Claudio Fagioli, Stefano de Pretis, Valerio Di Carlo, Nicoletta Landsberger, Daniele Zacchetti
Summary: CDKL5 is an important kinase involved in neuronal development and synapse formation. Decreased expression or activity of CDKL5 leads to severe neurodevelopmental disorders. This study investigates the translational regulation of CDKL5 and identifies important elements and factors involved. The findings provide insights into the physiological processes and pathogenesis related to CDKL5 deficiency disorder.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Review
Microbiology
Sahibzada Waheed Abdullah, Jin'en Wu, Xuefei Wang, Huichen Guo, Shiqi Sun
Summary: This review discusses the various types of IRESs found in Picornaviridae and the past and present discoveries regarding ITAFs and their mechanism of action. It also summarizes the importance of herbal extracts, drugs, and chemicals in controlling picornavirus translation and replication. The movement of ITAFs from the nucleus to viral replication factories is also discussed.
Article
Multidisciplinary Sciences
Geon-Woo Kim, Aleem Siddiqui
Summary: The m(6)A modification of the HCV RNA genome plays a crucial role in IRES-dependent translation, with the m(6)A reader protein YTHDC2 being essential for recognizing and supporting HCV translation initiation. This study highlights the functional significance of m(6)A modification and YTHDC2 in HCV IRES-dependent translation initiation, providing potential therapeutic targets for interfering with the infectious process.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Nicholas K. Clark, Meghan T. Harris, William B. Dahl, Zachary Knotts, Michael T. Marr II
Summary: IRES-mediated translation initiation requires a different set of factors compared to canonical cap-dependent translation. Cellular IRES mechanisms may resemble viral type III IRESes, allowing them to function with limited initiation factors under stress conditions.
MOLECULAR AND CELLULAR BIOLOGY
(2023)
Article
Immunology
Thomas Tipih, Mark Heise, Felicity Jane Burt
Summary: This study demonstrated the expression of CCHFV nucleoprotein by a Sindbis virus vector and its immunogenicity in mice. The induced immune response, predominantly Th1-type, was shown by the spectrum of cytokine production and antibody profile. Further studies in CCHFV-susceptible animals are needed to evaluate the protective efficacy of the vaccine.
Article
Virology
Zoltan Laszlo, Peter Pankovics, Gabor Reuter, Attila Csagola, Adam Balint, Mihaly Albert, Akos Boros
Summary: This study identified three novel picornaviruses and a potential new species Bopivirus B. Epidemiological investigation revealed that bopiviruses were most prevalent among <12-month-old ovine, caprine, and bovine. Phylogenetic analyses suggested the possibility of interspecies transmission of certain bopiviruses between ovine and caprine.
Article
Cell Biology
Kuanqing Liu, Daniel A. Santos, Jeffrey A. Hussmann, Yun Wang, Benjamin M. Sutter, Jonathan S. Weissman, Benjamin P. Tu
Summary: N-6-methyladenosine (m(6)A) is a conserved ribonucleoside modification that regulates RNA metabolism. This study found that tandem adenosines at the 3' end of 18S rRNA are also methylated, with significantly increased levels in response to sulfur starvation. Ribosomes with m(6)A were shown to have distinct translation preferences for sulfur metabolism genes.
Article
Allergy
M. Gil-Martinez, J. M. Rodrigo-Munoz, B. Sastre, J. A. Canas, R. Garcia-Latorre, N. Redondo, L. de la Fuente, P. Minguez, I Mahillo-Fernandez, J. Sastre, S. Quirce, M. L. Caballero, J. M. Olaguibel, V del Pozo
Summary: The aim of this study was to identify serum miRNAs that can phenotype asthma patients. Next-generation sequencing and RT-qPCR validation revealed that hsa-miR-26a-1-3p and hsa-miR-376a-3p were associated with eosinophilic and noneosinophilic asthma. Furthermore, the expression of these miRNAs was correlated with the pathogenesis of asthma.
JOURNAL OF INVESTIGATIONAL ALLERGOLOGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Engineering, Manufacturing
Raul Diaz, Khanh Nguyen, Francisco J. Montans, Miguel A. Sanz
Summary: Aluminum and high-strength steels are increasingly being used in sheet metal forming, but predicting springback effects accurately is more challenging than predicting plastic deformation. In this study, a novel hyperelastic-based multiplicative large strain elastoplasticity model is employed to achieve consistent and accurate predictions of springback effects.
INTERNATIONAL JOURNAL OF MATERIAL FORMING
(2022)
Article
Immunology
Lorena Forque, Mario Fernandez-Ruiz, Eliseo Albert, Estela Gimenez, Carolina Monzo, Javier Chaves, Natalia Redondo, Isabel Rodriguez-Goncer, Tamara Ruiz-Merlo, Patricia Parra, Amado Andres, Jose Maria Aguado, David Navarro
Summary: This study investigates the genetic diversity and clinical correlates of the Anelloviridae family after kidney transplantation. The dominant genus was Alphatorquevirus, followed by Betatorquevirus and Gammatorquevirus. Changes in the predominant genus were observed in some patients. Overall, the levels of Anelloviridae DNA increased after transplantation and were associated with the use of T-cell depleting agents. However, there were no associations between the dominant genus or total Anelloviridae DNA levels and clinical outcomes.
Article
Hematology
Catia Simoes, Maria-Carmen Chillon, David Martinez-Cuadron, Maria-Jose Calasanz, Maria-Belen Vridiales, Iria Vazquez, Montserrat Hernandez-Ruano, Benat Ariceta, Paula Aguirre-Ruiz, Leire Burgos, Diego Alignani, Sarai Sarvide, Sara Villar, Ana Alfonso Pierola, Felipe Prosper, Rosa Ayala, Joaquin Martinez-Lopez, Juan Miguel Bergua Burgues, Susana Vives, Jose A. Perez-Simon, Maria Garcia-Fortes, Teresa Bernal del Castillo, Mercedes Colorado, Mayte Olave, Juan I. Rodriguez-Gutierrez, Jorge Labrador, Marcos Gonzalez, Jesus F. San-Miguel, Miguel angel Sanz, Pau Montesinos, Bruno Paiva
Summary: Clonal evolution in acute myeloid leukemia (AML) can be reconstructed using integrated multidimensional flow cytometry (MFC) immunophenotyping and fluorescence-activated cell sorting (FACS) with next-generation sequencing (NGS). Mutational analysis of dysplastic cells and leukemic blasts at the onset of AML identified stable, branching, and clonal evolution patterns. Techniques other than single-cell multiomics can be used to detect measurable residual disease (MRD) and genetic traits associated with treatment resistance in patients achieving complete response (CR). Whole-exome sequencing revealed different clonal involvement in dysplastic myeloerythropoiesis, leukemic transformation, and chemoresistance.
Article
Engineering, Civil
Ismael Ben-Yelun, Miguel Diaz-Lago, Luis Saucedo-Mora, Miguel Angel Sanz, Ricardo Callado, Francisco Javier Montans
Summary: Applications of Structural Health Monitoring (SHM) combined with Machine Learning (ML) techniques enhance real-time performance tracking and increase structural integrity awareness of civil, aerospace and automotive infrastructures. In this paper, a novel ML algorithm is developed to predict fatigue damage in composite materials using the signals of Lamb Waves and other meaningful features. The proposed algorithm achieves good accuracy and computation times comparable to other ML algorithms.
ENGINEERING STRUCTURES
(2023)
Article
Virology
Mario Fernandez-Ruiz, Natalia Redondo, Patricia Parra, Tamara Ruiz-Merlo, Isabel Rodriguez-Goncer, Natalia Polanco, Esther Gonzalez, Francisco Lopez-Medrano, Rafael San Juan, David Navarro, Amado Andres, Jose Maria Aguado
Summary: This study compares two methods (intracellular cytokine staining and interferon-gamma release assay) in predicting immune protection among kidney transplant recipients. The results suggest that intracellular cytokine staining performs slightly better in predicting immune protection at the time of discontinuation of prophylaxis.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Chemistry, Physical
Ismael Ben-Yelun, Guillermo Gomez-Carano, Francisco J. San Millan, Miguel Angel Sanz, Francisco Javier Montans, Luis Saucedo-Mora
Summary: The goal of this research is to introduce a novel tunable 3D metamaterial that can reproduce a wide spectrum of 3D auxetic and non-auxetic Poisson's ratios and Young's moduli. By changing the connectivity and position of the cell's internal nodes, this metamaterial can accommodate different spatial curvatures and boundaries. This wide range of variation is achieved through simple spatial triangularization and allows for better adaptability to component design boundaries and shapes.
Article
Mathematics, Applied
Luis Saucedo-Mora, Ismael Ben-Yelun, Hugo Garcia-Modet, Miguel Angel Sanz-Gomez, Francisco Javier Montans
Summary: 3D printing is changing the design and manufacturing of high value industrial components, pursuing a macro-micro structural optimization of architected metamaterials to customize mechanical properties. Topology optimization techniques facilitate the design of microstructures and overall component topology. A novel topology optimization algorithm is proposed, aiming to homogenize the strain level by minimizing its standard deviation, without imposing constraints and reducing external parameters. The proposed technique, working directly with moduli, is suitable for material-component design using functionally graded metamaterials and can be extended to non-linear isotropic materials.
FINITE ELEMENTS IN ANALYSIS AND DESIGN
(2023)
Article
Immunology
Mario Fernandez-Ruiz, Estefani Garcia-Rios, Natalia Redondo, Isabel Rodriguez-Goncer, Tamara Ruiz-Merlo, Patricia Parra, Virginia Sandonis, Francisco Lopez-Medrano, Rafael San Juan, Esther Gonzalez, Natalia Polanco, Amado Andres, David Navarro, Jose Maria Aguado, Pilar Perez-Romero
Summary: This study investigates the impact of cytomegalovirus (CMV)-specific antibodies on infection in kidney transplant recipients. The results suggest that CMV-AbNEIs do not have a protective role in preventing CMV infection.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Immunology
Mario Fernandez-Ruiz, Beatriz Sanchez Moreno, Javier Santiago Almeda, Isabel Rodriguez-Goncer, Tamara Ruiz-Merlo, Natalia Redondo, Francisco Lopez-Medrano, Rafael San Juan, Amado Andres, Jose Maria Aguado
Summary: Previous statin therapy had no apparent protective effect on the outcome of post-transplant bloodstream infection among kidney transplant recipients. The time to clinical cure and secondary outcomes such as fever resolution, ICU admission or renal replacement therapy requirement, and 30-day all-cause mortality did not differ significantly between statin and non-statin groups.
TRANSPLANT INFECTIOUS DISEASE
(2023)
Article
Immunology
Blanca D. Lopez-Ayllon, Ana de Lucas-Rius, Laura Mendoza-Garcia, Transito Garcia-Garcia, Raul Fernandez-Rodriguez, Jose M. Suarez-Cardenas, Fatima Milhano Santos, Fernando Corrales, Natalia Redondo, Federica Pedrucci, Sara Zaldivar-Lopez, Angeles Jimenez-Marin, Juan J. Garrido, Maria Montoya
Summary: The study found that SARS-CoV-2 encodes eleven accessory proteins in its genome, and their roles during infection are not fully understood. Transcriptomics analysis revealed that WNT5A and IL11 were significantly up-regulated in A549 cells expressing certain accessory proteins from SARS-CoV-2. Bioinformatics analysis and functional assays confirmed the involvement of WNT5A and IL11 in pulmonary fibrosis, and altered profibrotic gene expression was observed in lung cell lines infected with SARS-CoV-2 and COVID-19 patients' lung biopsies. These findings suggest that targeting these accessory proteins could be a potential therapeutic approach against COVID-19.
FRONTIERS IN IMMUNOLOGY
(2023)
Letter
Surgery
Mario Fernandez-Ruiz, Patricia Parra, Tamara Ruiz-Merlo, Natalia Redondo, Isabel Rodriguez-Goncer, Amado Andres, Jose Maria Aguado
TRANSPLANT INTERNATIONAL
(2023)
Article
Transplantation
Mario Fernandez-Ruiz, Patricia Almendro-Vazquez, Natalia Redondo, Tamara Ruiz-Merlo, Sandra Abella, Adan Somoza, Francisco Lopez-Medrano, Rafael San Juan, Carmelo Loinaz, Amado Andres, Estela Paz-Artal, Jose Maria Aguado
Summary: The BA.4/BA.5-adapted bivalent booster vaccine generated strong subvariant-specific responses among SOT recipients, but the booster-induced cell-mediated immunity remained lower than in immunocompetent individuals.
TRANSPLANTATION DIRECT
(2023)
Article
Multidisciplinary Sciences
Natalia Redondo, Isabel Rodriguez-Goncer, Patricia Parra, Francisco Lopez-Medrano, Esther Gonzalez, Ana Hernandez, Hernando Trujillo, Tamara Ruiz-Merlo, Rafael San Juan, Maria Dolores Folgueira, Amado Andres, Jose Maria Aguado, Mario Fernandez-Ruiz
Summary: This study assessed the impact of different single nucleotide polymorphisms in genes related to innate immune responses on the risk of BKPyV viremia after kidney transplantation. It found a significant association between certain SNPs in TLR3, CD209, and IL10 genes and the development of BKPyV viremia.
SCIENTIFIC REPORTS
(2022)
Article
Peripheral Vascular Disease
Isabel Rodriguez-Goncer, Laura Corbella, David Lora, Natalia Redondo, Francisco Lopez-Medrano, Eduardo Gutierrez, Angel Sevillano, Ana Hernandez Vicente, Rafael San-Juan, Tamara Ruiz-Merlo, Patricia Parra, Esther Gonzalez, Maria Dolores Folgueira, Amado Andres, Jose Maria Aguado, Mario Fernandez-Ruiz
Summary: This study aimed to investigate whether cytomegalovirus (CMV) infection increases the risk of cardiovascular complications after kidney transplantation (KT). The analysis of 465 KT recipients revealed an association between CMV viremia and the incidence of post-transplant atherosclerotic (PAEs) and thrombotic events (PTEs), but this association was not significant after adjusting for various factors.
ATHEROSCLEROSIS PLUS
(2022)