Article
Chemistry, Physical
Huijuan Xu, Ruizheng Hou, Tong Tong, Hongrong Li
Summary: This study develops a kinetic model that is quantitatively validated by experimental data, revealing the physical mechanism of kinesin stepping and its directionality regulated by a biased diffusion process. The results suggest that kinesin directionality is optimized through fulfilling a thermodynamic constraint.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Biology
Alina Goldstein-Levitin, Himanshu Pandey, Kanary Allhuzaeel, Itamar Kass, Larisa Gheber
Summary: This study analyzed intracellular functions and motile properties of neck-linker variants of the bi-directional kinesin-5 motor Cin8. The results showed that exact N-latch stabilization during NL docking is critical for the function of bi-directional kinesin-5 Cin8.
Article
Chemistry, Physical
Kornel Rortocki, Filip Formalik, Volodymyr Bon, Anna Krawczuk, Piotr Goszczycki, Bogdan Kuchta, Stefan Kaskel, Dariusz Matoga
Summary: In this study, control over the adsorption properties of a flexible platform was achieved by incorporating halogen substituents, providing valuable guidance for the further development of flexible and adaptable materials.
CHEMISTRY OF MATERIALS
(2022)
Article
Chemistry, Physical
Shu-Xia Liu, Gang Lu, Hui Zhang, Yi-Zhao Geng, Qing Jix
Summary: Kinesin-1 moves along a microtubule with two motor heads dimerized by neck linkers and a coiled-coil stalk, building up internal strain essential for coordination. The unique sequence of Kinesin-1's NCC enhances its antiunwinding ability thanks to the hydrophobic pressure, while a buckle structure formed by tyrosines provides the shearing force needed for mechanical stability.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2021)
Article
Chemistry, Physical
Michael P. Howard, Zachary M. Sherman, Adithya N. Sreenivasan, Stephanie A. Valenzuela, Eric V. Anslyn, Delia J. Milliron, Thomas M. Truskett
Summary: This study demonstrates that the flexibility of the linker plays a key role in determining the phase behavior and structure of the colloid-linker mixture. Different flexibilities of the linker lead to different mixture behaviors, with flexible and rigid linker mixtures phase-separating at low colloid volume fractions, while semiflexible linker mixtures do not. The flexibility of the linker also influences the spacing between linked colloids, suggesting strategies for designing gels with desired properties.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Multidisciplinary Sciences
Jessica E. Besaw, Jorg Reichenwallner, Paolo De Guzman, Andrejs Tucs, Anling Kuo, Takefumi Morizumi, Koji Tsuda, Adnan Sljoka, R. J. Dwayne Miller, Oliver P. Ernst
Summary: In this study, the crystal structure of Thermoplasmatales archaeon SG8-52-1 heliorhodopsin (TaHeR) was determined under low pH conditions. The structure revealed the accommodation of a chloride anion in the hydrophilic Schiff base cavity (SBC), stabilizing the protonated retinal Schiff base. Computational studies further supported an intramolecular signaling transduction pathway, with allosteric network changes propagating from the perturbed SBC to the intracellular and extracellular space. These findings suggest the potential sensory role of TaHeR as a rhodopsin.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Physical
Matthias Mendt, Mariia Maliuta, Sebastian Ehrling, Friedrich Schwotzer, Irena Senkovska, Stefan Kaskel, Andreas Poeppl
Summary: The translation describes the improved performance of a flexible pillared layer metal-organic framework in gas adsorption and separation applications, as well as experimental results revealing the regulation mechanism through electron paramagnetic resonance studies.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Chemistry, Physical
Matthias Mendt, Mariia Maliuta, Sebastian Ehrling, Friedrich Schwotzer, Irena Senkovska, Stefan Kaskel, Andreas Poeppl
Summary: This paper introduces the importance of the metal-organic framework DUT-S(Ni) as a representative of flexible pillared layer MOFs and its performance as an adsorbent in gas delivery and separation applications. The study improved the detection of DUT-8(Ni) doped with Mn2+ through EPR, concluding that the EPR signal of Ni2+-Mn2+ PWs can be used to in situ detect the phase transition induced by CO2 adsorption.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Chemistry, Multidisciplinary
Timo Rabe, Erik Svensson Grape, Tobias A. Engesser, A. Ken Inge, Jonas Stroeh, Gitta Kohlmeyer-Yilmaz, Mohammad Wahiduzzaman, Guillaume Maurin, Frank D. Soennichsen, Norbert Stock
Summary: The reaction of V-shaped linker molecule 5-hydroxyisophthalic acid with Al or Ga nitrate under similar conditions produced metal-organic frameworks with different structures compositions, identified through in situ and ex situ studies using H-1 NMR spectroscopy and Rietveld refinement. The Al-MOF contained original linker and various nitration products, while the Ga-MOF mainly incorporated a specific nitration product. Unexpectedly, Ga-MOF crystallized in a structure different from the anticipated CAU-10 type, with higher stability confirmed through Density Functional Theory calculations and agreement with water adsorption isotherms.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Oncology
Alice Nicolai, Samanta Taurone, Simone Carradori, Marco Artico, Antonio Greco, Roberta Costi, Susanna Scarpa
Summary: K858, an inhibitor of motor kinesin Eg5, exerts significant antiproliferative and apoptotic effects on breast cancer, prostatic cancer, melanoma, and glioblastoma cells. It has been demonstrated that high levels of kinesin Eg5 expression are associated with poor prognosis in laryngeal carcinoma. This study investigated the anticancer activity of K858 on head and neck squamous cell carcinomas (HNSCCs), which are known to be unresponsive to therapy. The findings suggest that K858 inhibits cell replication, blocks the cell cycle in the G2 phase, induces apoptosis, and attenuates the malignant phenotype, making it a potential therapeutic option for HNSCCs.
INVESTIGATIONAL NEW DRUGS
(2022)
Article
Chemistry, Physical
Svetlana Kucher, Christina Elsner, Mariya Safonova, Stefano Maffini, Enrica Bordignon
Summary: The study demonstrates the feasibility of using double electron-electron resonance (DEER) technique to measure distances between protein sites at very low protein concentrations, providing a new approach for studying conformational changes of biomolecules.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Chemistry, Physical
C. Blake Wilson, Mian Qi, Songi Han, Mark S. Sherwin
Summary: Favorable relaxation processes, high-field spectral properties, and biological compatibility have made spin-7/2 Gd3+-based spin labels an increasingly popular choice for protein structure studies. However, the mechanisms of high-field relaxation and decoherence in half-integer high-spin systems like Gd3+ are still not well understood. This study presents a comprehensive model of high-field, high-spin decoherence and provides insights into the design of experiments using Gd3+ as spin probes or relaxivity agents.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Inorganic & Nuclear
Xiaoyan Cheng, Lirong Guo, Hongyu Wang, Jinzhong Gu, Ying Yang, Marina V. Kirillova, Alexander M. Kirillov
Summary: In this study, 4,4'-(Pyridine-3,5-diyl)dibenzoic acid (H2pdba) was used as a versatile linker to assemble various metal coordination polymers. Among the synthesized compounds, the 3D MOF 4 exhibited the best catalytic performance for the Knoevenagel condensation reaction, resulting in high yields of substituted dinitrile products.
INORGANIC CHEMISTRY
(2022)
Article
Chemistry, Inorganic & Nuclear
Xiaoyan Cheng, Lirong Guo, Hongyu Wang, Jinzhong Gu, Ying Yang, Marina V. Kirillova, Alexander M. Kirillov
Summary: The study explores the use of H2pdba as a versatile linker for assembling various metal coordination polymers, with particular focus on the catalytic potential of the resulting polymers in Knoevenagel condensation reactions. The 3D MOF 4 showed the best catalytic performance, leading to high product yields in the condensation reaction.
INORGANIC CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Francesco Torricella, Letizia Barbieri, Virginia Bazzurro, Alberto Diaspro, Lucia Banci
Summary: This article presents a novel protocol that delivers proteins into eukaryotic cells through mild thermal stimulation, allowing for the study of biomolecules in their natural environment. The data demonstrates the effectiveness of this method in delivering proteins to mammalian cells and achieving concentrations suitable for biophysical analysis, such as double electron electron resonance (DEER) measurements for protein conformation characterization.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)