Journal
JOURNAL OF MICROSCOPY
Volume 251, Issue 1, Pages 77-83Publisher
WILEY
DOI: 10.1111/jmi.12046
Keywords
3D Fourier analysis; rat cervix; second-harmonic generation; 3D imaging
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Funding
- National Science Foundation CAREER award [DBI 09-54155]
- National Institutes of Health [R21HD058705]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0954155] Funding Source: National Science Foundation
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We present the application of Fourier transform-second-harmonic generation (FT-SHG) imaging to evaluate the arrangement of collagen fibers in five nonpregnant rat cervices. Tissue slices from the mid-cervix and near the external orifice of the cervix were analyzed in both two-dimensions (2D) and three-dimensions (3D). We validate that the cervical microstructure can be quantitatively assessed in three dimensions using FT-SHG imaging and observe collagen fibers oriented both in and out-of-plane in the outermost and the innermost layers, which cannot be observed using 2D FT-SHG analysis alone. This approach has the potential to be a clinically applicable method for measuring progressive changes in collagen organization during cervical remodeling in humans.
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