Journal
JOURNAL OF MICROSCOPY
Volume 242, Issue 2, Pages 148-156Publisher
WILEY
DOI: 10.1111/j.1365-2818.2010.03448.x
Keywords
Multiphoton fluorescence excitation microscopy; refractive index heterogeneity; refractive index mismatch; scattering; spherical aberration; two-photon microscopy
Categories
Funding
- NIH [P30 DK 079312-01]
Ask authors/readers for more resources
P>Although multiphoton fluorescence excitation microscopy has improved the depth at which useful fluorescence images can be collected in biological tissues, the reach of multiphoton fluorescence excitation microscopy is nonetheless limited by tissue scattering and spherical aberration. Scattering can be reduced in fixed samples by mounting in a medium whose refractive index closely matches that of the fixed material. Using optical 'clearing', the effects of refractive index heterogeneity on signal attenuation with depth are investigated. Quantitative measurements show that by mounting kidney tissue in a high refractive index medium, less than 50% of signal attenuates in 100 mu m of depth.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available