Journal
JOURNAL OF MICROSCOPY
Volume 234, Issue 1, Pages 95-102Publisher
WILEY
DOI: 10.1111/j.1365-2818.2009.03143.x
Keywords
Cochlea; ganglion cell counting; osmium stain; synchrotron radiation; X-ray micro tomography
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The combination of osmium tetroxide staining and high-resolution tomographic imaging using monochromatic X rays allows visualizing cellular structures of the human inner ear, that is, the organ of Corti, the stria vascularis and further soft tissues of the membranous labyrinth, in three-dimensional space with isotropic micrometre resolution. This approach permits to follow the course of nerve fibre bundles in a major part of the specimen and reveals the detailed three-dimensional arrangement of individual ganglion cells with distinct nuclei by means of X-ray tomography for the first time. The non-destructive neuron cell counting in a selected volume of 125 mu m x 800 mu m x 600 mu m = 0.06 mm(3) gives rise to the estimate that 2000 ganglion cells are present along 1 mm organ of Corti.
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