Journal
JOURNAL OF MICROSCOPY
Volume 232, Issue 3, Pages 463-475Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2818.2008.02141.x
Keywords
3d reconstruction; biopolymer network; collagen gel; confocal microscopy; image processing
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Funding
- NIH [R01 CA085139-01A2]
- Institute for Mathematics and its Applications
- Swiss National Science Foundation
- NSF
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The geometric structure of a biopolymer network impacts its mechanical and biological properties. In this paper, we develop an algorithm for extracting the network architecture of three-dimensional (3d) fluorescently labeled collagen gels, building on the initial work of Wu et al., (2003). Using artificially generated images, the network extraction algorithm is then validated for its ability to reconstruct the correct bulk properties of the network, including fiber length, persistence length, cross-link density, and shear modulus.
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