Article
Chemistry, Analytical
Furui Jin, Danke Xu
Summary: The mi-CHA method detects miRNAs by changing the conformation of hairpin structured DNA probes on the chip surface, showing high sensitivity and specificity for clinical diagnosis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Dario Sanchez Martin, Reinier Oropesa-Nunez, Teresa Zardan Gomez de la Torre
Summary: Pathogen detection is of increasing importance, and nucleic acid detection techniques provide a reliable method to identify and detect pathogens. Magnetic sensors and magnetic labels have gained interest due to their simplicity, low cost, and versatility. In this study, we used the rolling circle amplification (RCA) technique combined with oligo-functionalized magnetic nanoparticles to detect RCA products by measuring the relaxation frequency change of the nanoparticles. By optimizing the use of micrometer-sized beads, we reduced the RCA time for faster detection and increased the detection sensitivity. Furthermore, our assay exhibited high specificity by only binding to amplified DNA that matched their labels. Overall, this research paves the way for a simple bioassay that can be used in outbreak settings and clinics worldwide without expensive laboratory equipment.
Article
Biotechnology & Applied Microbiology
Shijie Xu, Jian Chen, Fang Yang, Zhihao Yang, Jianrong Xu, Lanyue Wang, Lina Bian, Lihua Liu, Xiaoyu Zhao, Yunshan Zhang
Summary: This study proposes a powerful and reliable SNP detection platform that combines DNA walking reaction and magnetic bead separation. It can accurately and sensitively detect the relationship between individual genetic variations and carcinogenesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Luisa Galati, Jean-Damien Combes, Florence Le Calvez-Kelm, Sandrine McKay-Chopin, Nathalie Forey, Mathis Ratel, James McKay, Tim Waterboer, Lea Schroeder, Gary Clifford, Massimo Tommasino, Tarik Gheit
Summary: Human papillomavirus (HPV) circulating tumor DNA (HPV ctDNA) has been proposed as a biomarker for the detection and monitoring of HPV-related cancers. In this study, a bead-based HPV genotyping assay (E7-MPG) was evaluated for its performance in detecting HPV16 ctDNA in plasma samples from women diagnosed with HPV16-positive cervical cancer and premalignant lesions. The sensitivity of the E7-MPG test for HPV16-positive cervical cancer detection was found to be higher than that of droplet digital PCR (ddPCR), and the combination of HPV16 ctDNA and E6 antibodies increased the sensitivity for detection. These results suggest that E7-MPG can serve as a sensitive tool for ctDNA detection of HPV16-positive cervical cancer, and the addition of E6 antibodies improves the detection of cancer.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Analytical
Catarina R. F. Caneira, Denis R. Santos, Virginia Chu, Joao P. Conde
Summary: Recently, there has been a growing demand for rapid diagnosis of diseases, which has led to the development of new DNA detection platforms. However, the potential risk of misdiagnosis has hindered their market acceptance. In this study, a regenerable bead-based microfluidic device with integrated thin-film photodiodes was developed for real-time monitoring of DNA molecular recognition. The system demonstrated high sensitivity and the potential for reusability, which can reduce costs and improve the accuracy of diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Yuhe Wang, Wuzhen Qi, Lei Wang, Jianhan Lin, Yuanjie Liu
Summary: A new magnetic extraction method combined with microfluidic PCR was developed for detecting Salmonella, with an extraction efficiency of over 90% and a detection limit of 10(2) CFU/mL, promising rapid, sensitive, and simultaneous detection of multiple foodborne pathogens.
Article
Biochemical Research Methods
Hamid Aghamohammadi, Kathryn E. Thomas, Sanjana Srikant, Jason Deglint, Alexander Wong, Mahla Poudineh
Summary: To address the need for rapid detection of cyanotoxins in water resources, an easy-to-use and miniaturized system was developed. A bead-based, competitive fluorescence assay was presented for multiplexed detection of microcystin-LR (MC-LR) and okadaic acid (OA). A reusable microfluidic device, called toxin-chip, was designed for automated detection, which included a micromixer for efficient mixing and a detection chamber for magnetic retainment of beads. Quantum dots (QDs) were used as reporter molecules to enhance sensitivity, and the emitted fluorescence signal was inversely proportional to the toxin concentration. The device showed a low detection limit for both MC-LR and OA, and demonstrated chemical specificity against potential interfering toxins. The toxin-chip holds promise as a versatile and simple tool for cyanotoxin detection.
Article
Chemistry, Multidisciplinary
Jeonghun Kim, So Yeon Ahn, Soong Ho Um
Summary: This study presents a novel DNA nanostructure for detecting cancer miRNA variants with temperature insensitivity and high selectivity. By optimizing parameters and considering thermodynamic stability, design guidelines for new sensing systems of miRNA variation were provided.
Article
Biophysics
Tomoyuki Taguchi, Machi Ishikawa, Momoko Ichikawa, Takashi Tadenuma, Yuko Hirakawa, Tomoko Yoshino, Yoshiaki Maeda, Hiyori Takeuchi, Daisuke Nojima, Takeo Tanaami, Tadashi Matsunaga, Tsuyoshi Tanaka
Summary: In this study, a novel DNA microarray system was developed that eliminates the need for labeling, amplification, or washing of target nucleic acid fragments. By using specially designed DNA probes, the system allows for quick and specific detection of DNA and RNA within a short period of time.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Computer Science, Artificial Intelligence
Xin Shen, Xiangjuan Yao, Huijie Tu, Dunwei Gong
Summary: A method of dynamic community detection based on parallel multi-objective evolutionary optimization is proposed in this paper, which can timely and accurately detect the evolution of communities in a software ecosystem.
KNOWLEDGE-BASED SYSTEMS
(2022)
Article
Chemistry, Analytical
Min Ling, Na Luo, Lanyu Cui, Yongqiang Cao, Xueping Ning, Jian Sun, Xiaoping Xu, Shengbin He
Summary: This article introduces an on-bead DNA synthesis system for amplifying protein signals, which can sensitively quantify protein biomarkers and has great potential for clinical applications.
Article
Chemistry, Multidisciplinary
Zhun Lin, Jie Zhang, Zhengyu Zou, Gen Lu, Minhao Wu, Li Niu, Yuanqing Zhang
Summary: This article introduces a new method for serological assay in COVID-19 patients, which achieves high throughput and sensitivity by integrating color-size dual-encoded beads and rolling circle amplification technology. The method can quantitatively detect COVID-19 antibodies and also detect inflammatory biomarkers. In addition, the detection range of this method is tunable and can be used to simultaneously measure more COVID-19 serological molecules differing by orders of magnitude.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Lydia Lehniger, Anne Rudloff, Sibyll Pollok, Norman Grosse, Kristin Wessel, Monique Brendel, Juergen Popp, Karina Weber
Summary: An innovative approach combining direct PCR, viability PCR, and nested PCR was established for rapid and reliable identification of Escherichia coli. The use of propidium monoazide (PMA) allowed discrimination between viable and heat-killed bacterial cells, enhancing sensitivity. The inclusion of microarray-based DNA hybridization assay provided quick and species-specific DNA detection of E. coli.
Article
Multidisciplinary Sciences
Samla Gauri Balakrishnan, Mohd Ridzuan Ahmad, Seyed Saeid Rahimian Koloor, Michal Petru
Summary: This study aims to simulate the extraction and separation of ctDNA from the blood plasma of cancer patients for early and effective cancer detection. The results showed efficient separation of ctDNA for early cancer management, with a high sensitivity and specificity in tracing ctDNA using a microfluidics platform and SPM bead particles.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Multidisciplinary Sciences
Cheng-Ping Kuan, Chia-Hsin Tsai, Ching-Shan Tseng, Tso-Chi Yang
Summary: A rapid, sensitive, and simultaneous detection method for BSV, BBTV, and CMV using a bead-based multiplex assay was developed in this study. The method was ten times more sensitive than traditional RT-PCR and could be applied to field collected leaf samples for banana virus detection.
Article
Engineering, Electrical & Electronic
Jihee Lee, Kyoung-Rog Lee, Benjamin E. Eovino, Jeong Hoan Park, Luna Yue Liang, Liwei Lin, Hoi-Jun Yoo, Jerald Yoo
Summary: The ASIC presented in this study is a high-efficiency solution for a miniaturized 3D ultrasound system, featuring a charge-recycling high-voltage transmitter and a dynamic-bit-shared analog-to-digital converter. It achieves low power consumption, calibration function, and adaptive gain control, making it suitable for applications like 3D ultrasound imaging.
IEEE JOURNAL OF SOLID-STATE CIRCUITS
(2021)
Article
Chemistry, Multidisciplinary
Renxiao Xu, Peisheng He, Guangchen Lan, Kamyar Behrouzi, Yande Peng, Dongkai Wang, Tao Jiang, Ashley Lee, Yu Long, Liwei Lin
Summary: A time- and cost-effective fabrication methodology for multilayer stretchable electronics has been developed using a two-mode mechanical cutting process. This method allows for the creation of complex patterns without using conventional photolithography-based processes, resulting in excellent electronic performances of the devices even after repetitive large deformations.
Article
Chemistry, Multidisciplinary
Tao Jiang, Wenying Qiu, Zhaoyang Li, Xing Ye, Yuhan Liu, Yushi Li, Xiaohao Wang, Junwen Zhong, Xiang Qian, Liwei Lin
Summary: The study utilized a flexible actuator system to achieve basic tactile feedback functions and generate output forces, developed programmable tactile sensation patterns, and demonstrated tactile-assisted navigation and tactile-based braille prototypes, showcasing potential applications in cognitive assistance through tactile feedbacks.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Engineering, Multidisciplinary
Zhongbao Wang, Yigen Wu, Dezhi Wu, Daoheng Sun, Liwei Lin
Summary: The study utilized an advanced printing process to deposit soft magnetic composites in an orderly manner, creating deformable actuators with fast response, untethered control, and harmless human-machine interactions under low-strength magnetic fields. By controlling the magnetic polarities of microparticles and utilizing the microscale Weissenberg effect (MWE) through a rotating needle, the research effectively delivered the high viscosity magnetic composites ink.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Chemistry, Analytical
Georgii Grigorev, Nikolay O. Nikitin, Alexander Hvatov, Anna Kalyuzhnaya, Alexander Lebedev, Xiaohao Wang, Xiang Qian, Georgii Maksimov, Liwei Lin
Summary: This paper presents a generative design methodology for a micro hydrodynamic single-RBC trap, aiming to improve the through-slit flowrates for effective cell trapping in microfluidics-based single-cell analysis. The design, validated with experimental data, utilizes an evolutionary algorithm to generate optimized L-shaped trapping slits, leading to increased flow velocities and improved trapping efficiency for cells of various sizes.
Article
Nanoscience & Nanotechnology
Liuyang Han, Wei Zeng, Ying Dong, Xiaohao Wang, Liwei Lin
Summary: This study presents a high-density and large-scale flexible pressure sensor array for mapping and detecting arterial and venous pulses simultaneously. The experimental results show that this system can acquire detailed information about both arterial and venous pulses simultaneously, indicating its wide range of potential applications.
ADVANCED ELECTRONIC MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Ruiqi Guo, Fanping Sui, Wei Yue, Zekai Wang, Sedat Pala, Kunying Li, Renxiao Xu, Liwei Lin
Summary: The geometric designs of MEMS devices play a crucial role in their physical properties and performance. This paper proposes the use of deep learning techniques to accelerate the MEMS design cycle by accurately predicting the physical properties of multiple design candidates with different geometric features. By representing the design candidates using pixelated black-and-white images and training a deep neural network, the physical properties of interest can be quickly calculated without traditional numerical tools. This approach significantly reduces computation time and promotes experience-free and data-driven MEMS structural designs.
MICROSYSTEMS & NANOENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Zhaoyang Li, Yuan Ma, Kaijun Zhang, Jun Wan, Dazhe Zhao, Yucong Pi, Gangjin Chen, Jiangfeng Zhang, Wei Tang, Liwei Lin, Junwen Zhong
Summary: The paper introduces a wearable vibrotactile actuator that can generate mechanical stimulation on the human skin for various human-machine interaction applications. The actuator is characterized by excellent air permeability, low preload requirements, high output sensitivity, and good mechanical durability. Demonstrations include a wireless haptic feedback glove and large size actuators providing tactile feedback for English characters and parts of the human body. The proposed system opens up new possibilities for applications in metaverse, teleoperation, smart textiles, and robotics.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Electrical & Electronic
Liuhao Wu, Jiaqi Guo, Rucheng Jiang, Yande Peng, Han Wu, Jiamin Li, Yang Luo, Liwei Lin, Jerald Yoo
Summary: The article discusses the solution to the 3D depth sensing problem in drone vision and navigation. By developing a UIS ASIC with a specific image reconstruction scheme and design, real-time wireless 3D image streaming under different conditions has been successfully achieved.
IEEE JOURNAL OF SOLID-STATE CIRCUITS
(2022)
Proceedings Paper
Engineering, Electrical & Electronic
Yu Long, Peisheng He, Yande Peng, Liwei Lin
Summary: In this research, a thermoelectric energy harvester based on an ion-conductive ionogel is presented, which exhibits self-healing capability, ultrahigh Seebeck coefficient, and stable performance. It has the potential to convert the temperature differences between the human body/skin and the environment into electricity.
2022 IEEE 35TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS CONFERENCE (MEMS)
(2022)
Article
Materials Science, Multidisciplinary
Fanping Sui, Ruiqi Guo, Zhizhou Zhang, Grace X. Gu, Liwei Lin
Summary: This paper introduces the concept of digital materials and their application in composite material design. Through a deep reinforcement learning scheme, an automated process for digital material design is achieved, resulting in improved design quality and significant computational advantages.
ACS MATERIALS LETTERS
(2021)
Proceedings Paper
Engineering, Electrical & Electronic
Ruiqi Guo, Renxiao Xu, Zekai Wang, Fanping Sui, Liwei Lin
Summary: Machine learning is used to accelerate the MEMS design process by training neural networks on pixelated binary 2D images of candidate designs. The neural network can quickly and accurately identify vibrational modes and calculate frequencies, outperforming common FEA software by a factor of 4000 with a high accuracy of around 98%. The method is demonstrated using circular disk resonators as an example, showcasing the efficiency and effectiveness of the approach.
2021 34TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2021)
(2021)
Proceedings Paper
Engineering, Electrical & Electronic
Peisheng He, Yande Peng, Liwei Lin
Summary: This study presents a self-healable, flexible, and multimodal sweat sensor capable of detecting important indicators for skin and metabolic disorders. It maintains sensitivity under large strain and can recover conductivity and sensitivity through self-healing, making it suitable for wearable health monitoring systems that often encounter accidental damages.
2021 34TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2021)
(2021)
Proceedings Paper
Engineering, Electrical & Electronic
Sedat Pala, Zhichun Shao, Yande Peng, Liwei Lin
Summary: A phase-shift cancellation scheme has been proposed to reduce the ring down period of ultrasonic transducers and increase axial resolutions. Experimental results demonstrate that this scheme effectively shortens the ring down period and improves axial distance sensing resolution for both single elements and arrays.
2021 34TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2021)
(2021)
Proceedings Paper
Engineering, Electrical & Electronic
Sedat Pala, Zhichun Shao, Yande Peng, Liwei Lin
Summary: This study demonstrates the use of non-contact ultrasonic waves for delivering tactile sensations through a dual-electrode bimorph piezoelectric micromachined transducer. Experimental results show that the best tactile sensation on human fingers is achieved when simulating 100 Hz signals with high frequency ultrasonic waves, and strong haptic sensations are reported up to 10 cm away from the transducers.
2021 34TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2021)
(2021)
