Journal
JOURNAL OF MICROELECTROMECHANICAL SYSTEMS
Volume 20, Issue 4, Pages 800-810Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/JMEMS.2011.2159092
Keywords
Bacteriorhodopsin (bR); molecular electronics; optical device fabrication; photocells; proton pump
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Funding
- Natural Sciences and Engineering Research Council of Canada
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The fabrication of dry ultrathin photoelectric layers is described in this paper. The self-assembled monolayer of oriented purple membrane (PM) patches from bacteriorhodopsin (bR) is created on a bio-functionalized gold (Au) surface using a biotin molecular recognition technique. The biotin enables the extracellular side of the bR PMs to be accurately labeled. During the biochemical immobilization process, the biotinylated alkylthiols modify the Au surface using HS terminals of the thiols and affix the labeled bR to the functionalized surface using streptavidin-biotin interactions. An optically transparent indium tin oxide (ITO) electrode is then placed on top of the finished assembly to complete the circuit of the photocell for testing and performance verification. An experimental study shows that the proposed self-assembly method can produce a 12.33 nm thick photoelectric layer that generates nearly 0.54 mV/(mW . cm(2)) when exposed to a 568-nm laser beam. In contrast, other immobilization techniques such as electric field sedimentation produce dry bR films that are more than 10 mu m thick. These ultrathin photoelectric layers can be used to create nanoscale light sensors and photocells.
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