Article
Medicine, General & Internal
Pei-Chin Lin, Chia-Ling Chang, Yi-Hua Chung, Chih-Chun Chang, Fang-Yeh Chu
Summary: Blood volume and number of culture sets are important factors affecting blood culture positivity rate. The study finds that total blood culture volume and diagnosis of sepsis are critical factors influencing positivity rate. However, the proportion of blood culture bottles with optimal blood volume is low.
Article
Medicine, General & Internal
Kyoungbo Kim, Sunggyun Park
Summary: Several manufacturers have developed systems to automatically measure the amount of blood in culture bottles. We compared the volumes measured automatically by the Virtuo instrument (bioMerieux, France) and those calculated by weighing the bottles. The mean height-based volume was approximately 0.67 mL greater than the weight-based volume, particularly in anaerobic culture bottles. Foam did not have a significant effect. The LOD for the automatic height-based volume of equine blood was 0.2-0.4 mL and the 5 mL cut-off was 4-4.2 mL.
Editorial Material
Microbiology
Blake W. Buchan
Summary: Automated continuous monitoring blood culture systems play a crucial role in clinical microbiology laboratories, but their core technologies and performance characteristics have not changed significantly since the 1990s. A new study found that the introduction of a blood culture system with enhanced organism recovery and faster detection time had an impact on the management of patients with S. aureus bacteremia, but did not affect patient outcomes overall.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Alina-Ioana Andrei, Gabriel-Adrian Popescu, Mona Argentina Popoiu, Alexandru Mihai, Daniela Talapan
Summary: The study found that during the COVID-19 pandemic, there was a significant increase in blood contamination rates and a decrease in the number of blood cultures collected. Although the proportion of identified pathogens remained stable, there was a decrease in invasive bacterial/fungal infections and a change in species distribution.
Article
Radiology, Nuclear Medicine & Medical Imaging
Julien Poletti, Michael Bach, Shan Yang, Raphael Sexauer, Bram Stieltjes, David C. Rotzinger, Jens Bremerich, Alexander Walter Sauter, Thomas Weikert
Summary: The purpose of this study was to develop a robust method for automated lung vessel segmentation and morphology analysis and apply it to a large chest CT dataset. The results showed that in COVID-19 and Influenza, the blood vessel volume is redistributed from small to large vessels in the lung. This study contributes to a better understanding of vascular changes in infectious lung diseases.
EUROPEAN JOURNAL OF RADIOLOGY
(2022)
Article
Infectious Diseases
Cherry Lim, Viriya Hantrakun, Nittaya Teerawattanasook, Pramot Srisamang, Prapit Teparrukkul, Nithima Sumpradit, Paul Turner, Nicholas Pj Day, Ben S. Cooper, Sharon J. Peacock, Direk Limmathurotsakul
Summary: The impact of low blood culture utilization rate on estimated proportions and incidence rates of antimicrobial-resistant infections is significant, especially for bacteria like Escherichia coli and Klebsiella pneumoniae. The timing of blood culture collection during parenteral antibiotic treatment also plays a significant role in the development of antibiotic resistance.
JOURNAL OF INFECTION
(2021)
Article
Chemistry, Analytical
Pireh Pirzada, David Morrison, Gayle Doherty, Devesh Dhasmana, David Harris-Birtill
Summary: Current methods of measuring heart rate and oxygen levels require physical contact, but this novel study proposes a system that utilizes commercial camera technology to measure these parameters without physical contact. The system measures frequencies from different wavelength regions and produces accurate results.
Article
Multidisciplinary Sciences
Mark Campbell, Roslyn Dakin, Symon Stowe, Kira Burton, Brianna Raven, Malitela Mapani, Jeff W. Dawson, Andy Adler
Summary: Restrictive asphyxia may be related to arrest-related deaths, and our study found that under weighted restraint, respiratory muscles have a greater burden, leading to changes in lung reserve volumes.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Franziska Schlenker, Peter Juelg, Jan Lueddecke, Nils Paust, Roland Zengerle, Tobias Hutzenlaub
Summary: cfDNA is a promising biomarker for cancer monitoring and infectious disease diagnostics. This study presents a new concept for automated cfDNA extraction using nanobeads in a centrifugal microfluidic LabDisk. By optimizing the incubation chamber geometry, frequency, and temperature protocols, the recovery rates of cfDNA were maximized. The LabDisk showed better reproducibility and comparable allele frequencies compared to manual approaches, making it a highly attractive solution for automated liquid biopsy cfDNA extraction.
Article
Medicine, General & Internal
Anja von Laer, Micheline Ahou N'Guessan, Fidele Sounan Toure, Kathrin Nowak, Karin Groeschner, Ralf Ignatius, Johannes Friesen, Sara Tomczyk, Fabian H. Leendertz, Tim Eckmanns, Chantal Akoua-Koffi
Summary: Implementing automated blood cultures in resource-limited settings in Africa is feasible and improves microbiological diagnostic performance. Automated blood cultures increased yield and shortened turnaround times. Training and mentorship of clinicians need to be intensified to enhance both the quantity and quality of blood cultures.
FRONTIERS IN MEDICINE
(2021)
Article
Microbiology
Salvatore Pasqua, Francesco Monaco, Francesca Cardinale, Simone Bonelli, Pier Giulio Conaldi, Danilo D'Apolito
Summary: Blood cultures of patients with invasive Aspergillosis often show negative results, indicating the lack of a good strategy for the cultivation and isolation of Aspergillus spp. However, a study found that a specific subculture approach was effective in recovering Aspergillus from positive culture bottles, suggesting it as a valid and faster method for culture and isolation.
Article
Infectious Diseases
Gauthier Pean de Ponfilly, H. Benmansour, V Manda, E. Lecorche, F. Mougari, A. L. Munier, S. Temim, R. Amarsy, H. Jacquier, E. Cambau
Summary: Implementing automatic loading of BC bottles with a 24/7 strategy not only shortened the time to diagnosis but also significantly increased the BSI diagnosis rate.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Microbiology
Ana Veronica Halperin, Juan Antonio Del Castillo Polo, Jose Luis Cortes-Cuevas, Maria Jose Cardenas Isasi, Mario Ampuero Morisaki, Robert Birch, Ana Maria Sanchez Diaz, Rafael Canton
Summary: Bloodstream infections are associated with high morbidity and mortality rates. Blood culture is the standard method for diagnosing bloodstream infections. This clinical trial compared the performances of two blood culture incubation systems and found that the Virtuo system provided shorter time to detection (TTD) and turnaround time (TAT) compared to the Bactec FX system.
MICROBIOLOGY SPECTRUM
(2022)
Article
Physiology
Alicen A. Whitaker, Stacey E. Aaron, Carolyn S. Kaufman, Brady K. Kurtz, Stephen X. Bai, Eric D. Vidoni, Robert N. Montgomery, Sandra A. Billinger
Summary: This study is the first to investigate the cerebrovascular and hemodynamic response to low-volume high-intensity interval exercise (HIIT) with 1-minute intervals in young healthy adults. The middle cerebral artery blood velocity (MCAv) did not decrease below baseline during low-volume HIIT, but it decreased below baseline immediately after HIIT and returned to resting values 30 minutes after HIIT. There were also differences in MCAv between sexes.
JOURNAL OF APPLIED PHYSIOLOGY
(2022)
Article
Engineering, Civil
Eunsoo Choi, Jongkwon Choi, Junwon Seo
Summary: This study investigated the effect of fiber content and recovery stress on the dynamic direct tensile resistances of reinforced mortar. Results showed that adding fiber content increased tensile resistances in static tests, while recovery stress enhanced post-cracking strength and reduced strain capacity. In dynamic tests, peak strength reduction was attributed to reinforced mortar sensitivity, with 1.5% fiber content specimens showing higher toughness and strain capacity compared to 1.0% fiber content specimens. Recovery stress had a greater effect on 1.0% fiber content specimens, and all resistance parameters were sensitive to strain rate, especially for the 1.5% fiber content specimens.
ENGINEERING STRUCTURES
(2022)