Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 23, Issue 4, Pages 555-559Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1208.08029
Keywords
Detection; diagnosis; quantitation; outer membrane lipoprotein lolB; Vibrio cholera
Categories
Funding
- National Institute of Environmental Research
- National Academy of Agricultural Science, Rural Development Administration, Republic of Korea [PJ006651]
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Vibrio cholerae O1 and O139 are the major serotypes associated with illness, and some V. cholera non-O1 and non-O139 isolates produce cholera toxin. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the species-specific detection and quantitation of V: cholera using a primer pair based on an outer membrane lipoprotein lolB gene for the amplification of a 195 bp DNA fragment. The qPCR primer set for the accurate diagnosis of V cholera was developed from publically available genome sequences. This quantitative PCR-based method will potentially simplify and facilitate the diagnosis of this pathogen and guide disease management.
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