Article
Agricultural Engineering
Yafei Zhang, Jianzheng Li, Jia Meng, Kai Sun, Han Yan
Summary: The introduction of neutral red as an electron mediator and the control of the polarized electrode significantly improved the production of butanol and hydrogen in the CEF system.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Marcelo Rivas-Astroza, Ivan Paredes, Karlo Guerrero, Silvia Mau, Julian Quintero, Juan Carlos Gentina, Raul Conejeros, German Aroca
Summary: A new kinetic model of Clostridium beijerinckii's Acetone-Butanol-Ethanol fermentation, considering three cell types, accurately replicates batch culture data. The model estimates cell type-specific kinetic parameters, which can improve the operation of ABE fermentation and provide a framework for studying acidogenic and solventogenic metabolic pathways.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Materials Science, Paper & Wood
Fuangfa Srisuk, Piyawat Chinwatpaiboon, Thanaphat Atjayutpokin, Akarin Boonsombuti, Ancharida Savarajara, Apanee Luengnaruemitchai
Summary: Using pretreated vetiver grass as a carrier for immobilized Clostridium beijerinckii in ABE fermentation showed great potential, with butanol and total ABE concentrations approximately 1.3 times higher than that of the free-cell system. The pretreatment resulted in structural changes of the vetiver grass, increasing crystallinity and surface area. The vetiver grass samples were characterized using various techniques including X-ray diffraction, FTIR spectroscopy, TGA, SEM, and surface area analyses.
Article
Engineering, Chemical
Patthranit Narueworanon, Lakkana Laopaiboon, Pattana Laopaiboon
Summary: The study focused on enhancing butanol production from sugarcane molasses using immobilized Clostridium beijerinckii, with Lotus stalk pieces as carriers. Optimization of carrier size and loading led to higher butanol production levels, with the optimal conditions being a carrier size of 4 mm and a loading ratio of 1:31 (w/v). Fermentation results showed improved butanol concentration, productivity and yield compared to using free cells, with a 24-hour immobilization time found to be appropriate. Fermentation in different types of reactors showed varying butanol production efficiencies, with screw-capped bottles and stirred-tank reactors (STR) achieving the highest levels.
Article
Biotechnology & Applied Microbiology
Tinuola Olorunsogbon, Yinka Adesanya, Hasan K. Atiyeh, Christopher Chukwudi Okonkwo, Victor Chinomso Ujor, Thaddeus Chukwuemeka Ezeji
Summary: This study provides evidence on the effectiveness of metabolically engineered C. beijerinckii NCIMB 8052 for fermenting LB-derived hydrolysates to produce acetone-butanol-ethanol (ABE). The engineered strain showed higher butanol and ABE production in non-detoxified hydrolysates compared to the wildtype strain, and even higher production in detoxified hydrolysates.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Sireethorn Khaonuan, Rattana Jariyaboon, Nikannapas Usmanbaha, Benjamas Cheirsilp, Nils-Kare Birkeland, Prawit Kongjan
Summary: Marine macroalgae, specifically Gracilaria tenuistipitata, showed the highest potential for butanol production through acetone-butanol-ethanol fermentation. Low-temperature hydrothermal pretreatment of G. tenuistipitata resulted in a high reducing sugar yield rate and ABE yield.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Microbiology
Petra Patakova, Jan Kolek, Katerina Jureckova, Barbora Branska, Karel Sedlar, Maryna Vasylkivska, Ivo Provaznik
Summary: Research on the response mechanisms of clostridia to butanol shock during acetone-butanol-ethanol (ABE) fermentation revealed the importance of fatty acid cyclopropanation and plasmalogen formation in cell membrane stabilization in the presence of butanol. After butanol shock, genes related to glucose uptake, glycolysis, and acidogenesis were downregulated, while solventogenesis genes were upregulated. Additionally, upregulation of heat shock protein genes and several putative butanol efflux pumps after butanol addition were observed.
Article
Biotechnology & Applied Microbiology
Julalak Buranaprasopchai, Nassapat Boonvitthya, Chompunuch Glinwong, Warawut Chulalaksananukul
Summary: This study reports the potential of utilizing Sato wastewater (STW) for butanol production. The strain CUEA02, isolated from natural sources, was capable of growing and producing butanol from various carbon sources, and could utilize diluted STW for ABE production. Adjusting the initial pH and adding yeast extract were found to enhance the butanol yield.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Agricultural Engineering
A. Valles, M. Capilla, F. J. Alvarez-Hornos, M. Garcia-Puchol, P. San-Valero, C. Gabaldon
Summary: The research found that optimizing NaOH pretreatment conditions can effectively increase the efficiency and yield of producing biobutanol from rice straw. The optimal conditions of 5% solid loading and 0.75% NaOH led to a biomass utilization rate of 77.6 g kg RS-1.
BIOMASS & BIOENERGY
(2021)
Article
Agricultural Engineering
Yafei Zhang, Jianzheng Li, Yang-chun Yong, Zhen Fang, Han Yan, Jiuling Li, Jia Meng
Summary: In this study, a novel cathodic electro-fermentation system mediated by methyl viologen was used to achieve selective and high-yield butanol production. The addition of butyric acid as a co-substrate further improved the production and selectivity of butanol. This approach holds great promise for cost-effective bio-butanol production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Energy & Fuels
Nazlihan Tekin, Sevgi Ertugrul Karatay, Gonul Donmez
Summary: The current study evaluates the effect of industrial tea waste (ITW) on biobutanol production and optimizes critical parameters. Using ITW as a feedstock can increase the yield and productivity of biobutanol under optimized conditions.
Article
Chemistry, Physical
Md. Ebrahim Khalil, Akanksha Jain, Kun Lin Yang, Gobinath Rajagopalan
Summary: The study found that adding rice bran hydrolysate and ferrous chloride to the culture medium of Clostridium beijerinckii G117 can enhance the assimilation of native crude glycerol and hydrogen production. By optimizing the medium conditions, a higher yield of hydrogen and cellular growth was achieved.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Review
Biotechnology & Applied Microbiology
Petra Patakova, Barbora Branska, Maryna Vasylkivska, Katerina Jureckova, Jana Musilova, Ivo Provaznik, Karel Sedlar
Summary: Solventogenic clostridia, although not a strictly defined group within the genus Clostridium, share common features such as being anaerobic, non-pathogenic, non-toxinogenic, and endospore forming bacteria. Their main metabolite is typically 1-butanol, but they can also produce other metabolites depending on species and culture conditions. Despite being used for industrial production in the past, they were replaced by more efficient chemical production methods. Transcriptome analysis is a valuable tool for gaining deeper insights into clostridial metabolism and life.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Chemistry, Physical
Yu. V. Litti, N. D. Khuraseva, A. V. Vishnyakova, E. A. Zhuravleva, A. A. Kovalev, D. A. Kovalev, V. A. Panchenko, S. N. Parshina
Summary: The hydrogen-producing bacteria, Clostridium butyricum (SP4) and Clostridium beijerinckii (SP6), were isolated from compost. They showed different biohydrogen-producing activities when using different types of sugars as substrates. The efficiency of converting wastewater to hydrogen by C. butyricum SP4 was slightly lower than that of C. beijerinckii SP6. The metabolic capabilities and kinetic parameters of these bacteria provide valuable information for optimizing the technology of biohydrogen production.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Biotechnology & Applied Microbiology
Cansu Birgen, Kristin F. Degnes, Sidsel Markussen, Alexander Wentzel, Havard Sletta
Summary: The study investigated the impact of different sugar mixtures on fermentation using the BioLector(R) system, demonstrating its suitability for high-throughput anaerobic cultivation studies. The results provide valuable insights for further research in industrial fermentations of lignocellulosic biomass.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biotechnology & Applied Microbiology
Nurzhan Kuanyshev, Christopher Rao, Bruce Dien, Yong-Su Jin
Summary: This study successfully demonstrated the targeted modification of Z. bailii using a CRISPR-Cas9 system to produce lactic acid without ethanol production. The engineered strain showed promise for further applications and fundamental studies in the species.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Yalin Li, Sarang S. Bhagwat, Yoel R. Cortes-Pena, Dongwon Ki, Christopher Rao, Yong-Su Jin, Jeremy S. Guest
Summary: The study utilized an open-source platform for the design, simulation, and evaluation of lignocellulosic lactic acid biorefineries, demonstrating the potential for producing competitive lactic acid and the possibility of further enhancing performance through technological advancements and process optimization. Sensitivity analyses were conducted to quantify the impact of fermentation unit and feedstock characteristics on the sustainability of the biorefinery, highlighting the importance of agile TEA/LCA in screening promising biorefinery designs and establishing a roadmap for continued development of bioproducts and biofuels.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Review
Biochemical Research Methods
Jae Won Lee, Sangdo Yook, Hyungi Koh, Christopher Rao, Yong-Su Jin
Summary: Efforts have been concentrated on reprogramming regulatory networks to enhance xylose utilization by engineered yeast and activating and enhancing xylose metabolism in oleaginous yeasts. The potential benefits of using xylose for the production of value-added products have also been demonstrated.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Seung-Oh Seo, Ting Lu, Yong-Su Jin, Hans P. Blaschek
Summary: This study characterized the solventogenic clostridia mutant BA101 and identified potential genetic targets for rational engineering of desired phenotypes through genome sequencing and comparative genomic analysis.
JOURNAL OF BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Lahiru N. Jayakody, Yong-Su Jin
Summary: Aldehydes are highly toxic to Saccharomyces cerevisiae, with compounds containing aldehyde functional groups posing a greater threat. Researchers have developed synthetic yeast cell factories to convert lignocellulosic substrates into valuable products, including aldehyde compounds, using novel molecular approaches to overcome aldehyde toxicity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Eun Ju Yun, Sora Yu, Young-Ah Kim, Jing-Jing Liu, Nam Kang, Yong-Su Jin, Kyoung Kim
Summary: This study investigates the prebiotic and anti-colon cancer activities of various agar-derived sugars, with agarotriose showing prebiotic activity and 3,6-anhydro-l-galactose demonstrating anti-colon cancer effects. These results provide important insights into the health benefits derived from red seaweeds.
Article
Biotechnology & Applied Microbiology
Minhye Shin, Heeyoung Park, Sooah Kim, Eun Joong Oh, Deokyeol Jeong, Clarissa Florencia, Kyoung Heon Kim, Yong-Su Jin, Soo Rin Kim
Summary: The deletion of GCR2 gene has been identified to have a significant impact on xylose fermentation in Saccharomyces cerevisiae, leading to the upregulation of PPP genes and downregulation of glycolytic genes, particularly under xylose conditions. Although there was no synergistic effect between Delta pho13 and Delta gcr2 in improving xylose fermentation, the results suggest that GCR2 is a novel knockout target for enhancing lignocellulosic ethanol production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Nam Kyu Kang, Jae Won Lee, Donald R. Ort, Yong-Su Jin
Summary: In this study, engineered Saccharomyces cerevisiae was utilized to produce malic acid from xylose, with increased malic acid yield and productivity achieved through a low-level xylose fed-batch strategy. This work suggests effective strategies for using xylose in the biological production of value-added products.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Jonghyeok Shin, Yong-Su Jin, Yong-Cheol Park, Jin-Byung Park, Young-Oh Lee, Sun-Ki Kim, Dae-Hyuk Kweon
Summary: In this study, it was found that introducing the influenza A virus M2 protein into E. coli can increase intracellular pH, enhance cell survival, and improve adaptation to various weak acids. This strategy can be utilized to develop efficient whole-cell biocatalysts and improve the production of specific products that are hindered by cytosolic acidification.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Igor A. Podolsky, Susanna Seppala, Haiqing Xu, Yong-Su Jin, Michelle A. O'Malley
Summary: The study identified a novel fungal SWEET transporter, NcSWEET1, from anaerobic fungi that can enhance glucose and xylose co-utilization. By constructing single cross-over chimeras and studying protein structure and function, enhanced variants were identified, offering potential applications in improving sugar transport efficiency.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Yerin Jin, Sora Yu, Jing-Jing Liu, Eun Ju Yun, Jae Won Lee, Yong-Su Jin, Kyoung Heon Kim
Summary: In this study, the probiotic yeast S. boulardii was engineered to produce health-beneficial neoagarooligosaccharides (NAOSs) by expressing an endo-type beta-agarase, BpGH16A, derived from a human gut bacterium Bacteroides plebeius. The results suggest that engineered S. boulardii can be considered a microbial cell factory for producing useful substances in the human gut, with successful production of health-benefiting NeoDP4 from agarose.
MICROBIAL CELL FACTORIES
(2021)
Article
Multidisciplinary Sciences
Liang Sun, Jae Won Lee, Sangdo Yook, Stephan Lane, Ziqiao Sun, Soo Rin Kim, Yong-Su Jin
Summary: The authors successfully engineered Baker's yeast to co-consume xylose and acetate for triacetic acid lactone production. This metabolic re-configuration boosted the synthesis of valuable bioproducts such as TAL.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Eun Ju Yun, Sora Yu, Na Jung Park, Yoonho Cho, Na Ree Han, Yong-Su Jin, Kyoung Heon Kim
Summary: The study reveals the cooperative actions of the two human gut symbionts in degrading agarose, the main carbohydrate of red seaweed, and generating monomeric sugar possessing anti-inflammatory activity. This provides a molecular basis for agarose degradation by gut bacteria.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Ming-Hsun Cheng, Bruce Stuart Dien, Yong-Su Jin, Stephanie Thompson, Jonghyeok Shin, Patricia J. Watson Slininger, Nasib Qureshi, Vijay Singh
Summary: This study investigated the process of pretreating, enzymatic hydrolysis, and fermentation of concentrated sorghum syrup to produce lipids and ethanol. The research identified the most suitable yeast strains for lipid production and engineered yeast strains for cofermentation.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Review
Biochemical Research Methods
Liang Sun, Yong-Su Jin
Summary: The microbial conversion of plant biomass into fuels and chemicals using engineered yeast has shown progress in efficiently converting xylose and co-utilizing it with glucose. Research investments have facilitated the simultaneous fermentation of xylose and glucose, with attention now turning towards improving xylose-utilizing efficiency and achieving robust co-fermentation under industrial conditions. Harnessing the advantageous traits of yeast xylose metabolism is expected to lead to the production of diverse fuels and chemicals.
BIOTECHNOLOGY JOURNAL
(2021)