Burkholderia denitrificans sp nov., Isolated from the Soil of Dokdo Island, Korea

A novel, Gram-negative, bacterial strain KIS30-44(T) was identified from wet forest soil collected on the Korean island of Dokdo. Growth of the strain was observed at 15-30 degrees C, pH 5-9, 0-3% NaCl, and 950 mM KNO3. KIS30-44(T) reduced nitrate to nitrogen gas. Analysis of the 16S rRNA gene sequence showed that KIS30-44(T) was phylogenetically related to Burkholderia sacchari, Burkholderia mimosarum, and Burkholderia oxyphila (98.1%, 98.0%, and 98.0% sequence similarity, respectively). The genomic G+C content was 63.5 mol%. KIS30-44(T) exhibited less than 52% DNA-DNA relatedness with the type strains of 9 closely related Burkholderia species. The major isoprenoid quinone was Q-8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminolipids. The major fatty acids in KIS30-44(T) were C-16:0, C-18:1 omega 7c and summed feature 3 (iso-C-15:0 2-OH and C-16:1 w7c), and the strain contained half the amount of C-17:0 cyclo found in the 9 closely related Burkholderia species. The results of these phenotypic, 16S rRNA gene sequence, DNA-DNA hybridization, and chemotaxonoinic data indicate that KIS30-44(T) represents a novel species within the genus Burkholderia, for which the name Burkholderia denitrificans (Type strain KIS30-44(T) = KACC 12733(T) = DSM 24336(T)) is proposed.