Review
Microbiology
John E. Cronan
Summary: This review provides an update on the research of Escherichia coli acetyl-CoA carboxylase (ACC), focusing on the structures, stoichiometry, regulation of ACC activity and expression.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2021)
Article
Microbiology
Pan Wang, Xin Wang, Ying Yin, Mingliang He, Wei Tan, Wenting Gao, Jianping Wen
Summary: The study found that inactivating the signal transduction protein GlnB can increase the production of ascomycin by enhancing the supply of precursors. Protein co-precipitation experiments and coupled enzyme activity assays revealed that the interaction between GlnB protein and the alpha subunit of acetyl-CoA carboxylase and propionyl-CoA carboxylase inhibited their activities.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Andrea L. Livieri, Facundo Colaccini, Martin A. Hernandez, Gabriela Gago, Hector M. Alvarez, Hugo Gramajo, Eduardo Rodriguez
Summary: In actinomycetes, acyl-CoA carboxylases (ACCs) are biotin-dependent enzymes with diverse structures and functions. Rhodococcus jostii RHA1 was found to have multiple putative ACC domains and subunits. Mutant strains with specific genes disrupted showed the importance of these genes in lipid biosynthesis and growth.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Buli Su, Peixuan Lai, Fan Yang, Anzhang Li, Ming-Rong Deng, Honghui Zhu
Summary: This study developed a novel method to increase the supply of acetyl-CoA in Saccharomyces cerevisiae and improve the production of downstream products. By optimizing metabolic pathways and conducting genetic engineering, the production of mevalonic acid was successfully increased, and further improvement was achieved by dynamically downregulating gene expression. The final strain produced a high yield of lycopene.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Minmin Wang, Matthew G. Garneau, Arati N. Poudel, Daniel Lamm, Abraham J. Koo, Philip D. Bates, Jay J. Thelen
Summary: The limiting subunit of plant acetyl-CoA carboxylase, identified as alpha-carboxyltransferase, was found to be responsible for the increase in both fatty acid synthesis rate and seed oil content when overexpressed in Arabidopsis and Camelina.
Article
Biology
Imtiaz Ali, Abbas Khan, Zhang Fa, Taimoor Khan, Dong-Qing Wei, Jianting Zheng
Summary: Acetyl-CoA carboxylase (ACC) plays a crucial role in polyketides biosynthesis and it is a promising drug target against obesity, cancer, microbial infections, and diabetes. However, the lack of knowledge about the relationship between ACC ligand-enzyme binding has hindered the development of ACC-specific therapeutics and unnatural polyketides. In this study, the crystal structure of AccB from Streptomyces antibioticus was determined to understand the substrate specificity and the molecular mechanism of acetyl-CoA recognition and processing. Key residues for substrate recognition were identified, and the atomic-level knowledge of substrate binding was provided, which will aid in the re-design of ACCs active site for efficient substrate specificity and diverse polyketide production.
COMPUTERS IN BIOLOGY AND MEDICINE
(2022)
Article
Geriatrics & Gerontology
Amy Zheng, Seong Eun Kwak, Jesper B. Birk, Edward B. Arias, Dominic Thorley, Jorgen F. P. Wojtaszewski, Gregory D. Cartee
Summary: This study investigated the impact of caloric restriction (CR) on AMP-activated protein kinase (AMPK) and its substrates in skeletal muscle of older animals. The results revealed that CR significantly increased the phosphorylation of AMPK and several AMPK substrates, suggesting a potential role of AMPK in the antiaging effects of CR.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Article
Agronomy
Tengfei Zhan, Xin Guo, Lu Ma, Shengyong Mao, Dengpan Bu
Summary: This study evaluated the effect of biotin and leucine on the synthesis of odd- and branched-chain fatty acids (OBCFA) in ruminants. The results showed that biotin promoted dry matter degradation, while leucine increased the concentration of branched-chain volatile fatty acids and valerate. In conclusion, biotin and leucine can be effective nutrition strategies to promote OBCFA synthesis.
Article
Chemistry, Medicinal
Tonghui Huang, Xin Wu, Shirong Yan, Tianya Liu, Xiaoxing Yin
Summary: A series of spiroketopyrazole derivatives were synthesized and evaluated for their anticancer activities as ACC inhibitors. Compound 7j showed the strongest enzyme inhibitory activity, while compound 7m exhibited potent anti-proliferative effects against various cancer cell lines, indicating its potential as a therapeutic agent for hepatocellular carcinoma.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ryo Mizojiri, Daisuke Tomita, Masako Sasaki, Yoshihiko Satoh, Yukiko Yamamoto, Hiroyuki Sumi, Hironobu Maezaki
Summary: By modifying the molecular length, a new ACC1-selective inhibitor 1i was discovered. Further chemical modification produced compound 1n with potent ACC1 selective inhibition and suitable PK profile for in vivo evaluations. This novel series of potent ACC1-selective inhibitors shows promise as both research tools and potential therapeutic agents.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Oncology
Fei-Yuan Yu, Qian Xu, Qi-Yao Wei, Hai-Ying Mo, Qiu-Hua Zhong, Xiao-Yun Zhao, Andy T. Y. Lau, Yan-Ming Xu
Summary: ACC2 is under-expressed in cancerous tissue and negatively correlated with tumor size, lymph-node metastases, and clinical stage in lung adenocarcinoma. Knocking down ACC2 promotes cell proliferation and migration, and affects the expression of cell cycle-related genes. Lung adenocarcinoma patients with under-expressed ACC2 have a worse prognosis.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2022)
Article
Microbiology
Tianyu Chen, Jing Yao, Keao Quan, Jingchen Xu, Xudong Hang, Qian Tong, Genyan Liu, Peipei Luo, Liping Zeng, Ganzhu Feng, Hongkai Bi
Summary: This study investigates the antifungal efficacy of firsocostat against Candida albicans. The combination of firsocostat with other antifungal agents shows synergistic effects in drug-sensitive and drug-resistant C. albicans strains. The study also demonstrates that firsocostat directly binds to C. albicans ACC and inhibits its enzymatic activity. These findings suggest that repurposing firsocostat holds promising potential in antifungal drug development and candidiasis treatment.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Siraprapa Siritutsoontorn, Witchuda Sukjoi, Steven W. Polyak, Chareeporn Akekawatchai, Sarawut Jitrapakdee
Summary: HLCS plays a crucial role in breast cancer growth and migration by promoting the biotinylation of carboxylases. Depletion of biotin or reduced expression of HLCS inhibits cell proliferation and migration, leading to apoptosis in breast cancer cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Wenyu Wu, Ruilin Chen, Yuanda Wan, Liren Li, Jiajia Han, Qiyun Lei, Zhipeng Chen, Shuwen Liu, Xingang Yao
Summary: Dengue fever is a common viral disease transmitted by mosquitoes, and there is a need for new drugs to combat it. Research suggests that inhibiting acetyl-CoA carboxylase (ACC) can effectively decrease the proliferation of the dengue virus and improve survival rates in infected mice.
Article
Biochemistry & Molecular Biology
Xin Wu, Yongbo Yu, Tonghui Huang
Summary: A potential ACC inhibitor was identified in this study, and a series of related compounds were synthesized for investigation, among which 6g showed the strongest ACC inhibitory activity, while 6l exhibited potent cytotoxicity. Preliminary mechanistic studies suggested that these compounds may act by reducing malonyl-CoA levels, arresting the cell cycle, and inducing apoptosis.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Atanas V. Demirev, Anamika Khanal, Bhishma R. Sedai, Si Kyu Lim, Min Kyun Na, Doo Hyun Nam
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2010)
Article
Microbiology
Atanas V. Demirev, Anamika Khanal, Nguyen Phan Kieu Hanh, Kyung Tae Nam, Doo Hyun Nam
JOURNAL OF MICROBIOLOGY
(2011)
Article
Neurosciences
Ha-Lim Song, Atanas Vladimirov Demirev, Na-Young Kim, Dong-Hou Kim, Seung-Yong Yoon
MOLECULAR AND CELLULAR NEUROSCIENCE
(2019)
Article
Neurosciences
Atanas Vladimirov Demirev, Ha-Lim Song, Mi-Hyang Cho, Kwangmin Cho, Jong-Jin Peak, Hyun Ju Yoo, Dong-Hou Kim, Seung-Yong Yoon
NEUROBIOLOGY OF DISEASE
(2019)
Article
Neurosciences
Kyu-Ho Shim, Soo-Hwan Kim, Joon Hur, Dong-Hou Kim, Atanas Vladimirov Demirev, Seung-Yong Yoon
NEUROBIOLOGY OF DISEASE
(2019)
Article
Biotechnology & Applied Microbiology
Lee Ji Seon, Miglena C. Vladimirova, Atanas V. Demirev, Bo Geum Kim, Si Kyu Lim, Doo Hyun Nam
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2008)
Article
Microbiology
Anna Yoo, Atanas V. Demirev, Ji Seon Lee, Sang Dal Kim, Doo Hyun Nam
JOURNAL OF MICROBIOLOGY
(2006)
Article
Microbiology
AV Demirev, CH Lee, BP Jaishy, DH Nam, DDY Ryu
FEMS MICROBIOLOGY LETTERS
(2006)