Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 103, Issue -, Pages 18-24Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2014.05.008
Keywords
Artificial wound bed; Multi-species wound biofilm model; Anti-biofilm substances; In vitro testing
Categories
Funding
- Technology Agency of the Czech Republic [TA03011029]
- Charles University in Prague, Faculty of Medicine in Hradec Kralove [SVV-2014-260058]
Ask authors/readers for more resources
Wound infections represent a major problem, particularly in patients with chronic wounds. Bacteria in the wound exist mainly in the form of biofilms and are thus resistant to most antibiotics and antimicrobials. A simple and cost-effective in vitro model of chronic wound biofilms applied for testing treatments and solid devices, especially wound dressings, is presented in this work. The method is based on the well-established Lubbock chronic wound biofilm transferred onto an artificial agar wound bed. The biofilm formed by four bacterial species (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa) was stable for up to 48 h post-transplant. The applicability of the model was evaluated by testing two common iodine wound treatments. These observations indicate that this method enables assessing the effects of treatments on established resilient wound biofilms and is clinically highly relevant. (C) 2014 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available