Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 102, Issue -, Pages 23-25Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2014.04.008
Keywords
Bacterial detachment; Flow cytometry; Seagrass-associated bacteria; Enumeration; Microbe; Seagrass
Categories
Funding
- University of Technology Sydney Climate Change Cluster
- CSIRO Flagship Marine and Coastal Carbon Biogeochemistry Cluster
- Marine and Coastal Carbon Biogeochemistry Cluster, CSIRO
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A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enumerating seagrass-associated bacteria with FCM. Using this approach we detected cell concentrations between 2.0 x 10(5) and 8.0 x 10(6) cells mg(-1) DW tissue. 2014 Elsevier B.V. All rights reserved.
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