Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 92, Issue 1, Pages 9-10Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2012.10.013
Keywords
Digital PCR; Real-time PCR; Copy number; Bacillus anthracis; Plasmids
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Funding
- United States Department of Homeland Security Science and Technology Directorate [HSHQDC-08-X-00843]
- U.S. Department of Energy [DE-AC06-76RLO]
- DHS ST [HSHQDC-08-X-00763]
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We evaluated digital PCR (dPCR) to directly enumerate plasmid and chromosome copies in three strains of Bacillus anthracis. Copy number estimates based on conventional quantitative PCR (qPCR) highlighted the variability of using qPCR to measure copy number whereas estimates based on direct sequencing are comparable to dPCR. (C) 2012 Elsevier B.V. All rights reserved.
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