Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 95, Issue 2, Pages 201-206Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2013.08.016
Keywords
Beaver-specific marker; Real-time PCR; Microbial source tracking; Waterborne pathogens
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Funding
- AAFC SAGES program
- NSERC Visiting Fellowship in Government Laboratories program
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In many settings wildlife can be a significant source of fecal pathogen input into surface water. The North American beaver (Castor canadensis) is a zoonotic reservoir for several human pathogens including Cryptosporidium spp. and Giardia spp. In order to specifically detect fecal pollution by beavers, we have developed and validated a beaver-specific Bacteroidales marker, designated Beapol01, based on the 16S rRNA gene. The marker is suitable for quantifying pollution using real-time PCR. The specificity and sensitivity of the marker was excellent Beaver signal was detected in water of a mixed-activity watershed harbouring this rodent. Overall, Beapol01 will be useful for a better understanding of fecal source inputs in drainage basins inhabited by the beaver. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.
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