Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 94, Issue 3, Pages 378-380Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2013.07.011
Keywords
Tannerella forsythia; Natural competence; Transformation; Mutagenesis
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Funding
- Japan Society for Promotion of Science [KAKENHI 23592722]
- Grants-in-Aid for Scientific Research [23592722] Funding Source: KAKEN
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We report the discovery of natural competence in Tannerella forsythia and its application to targeted chromosomal mutagenesis. Keeping T. forsythia in a biofilm throughout the procedure allowed efficient DNA uptake and allelic replacement This simple method is cost-effective and reproducible compared with the conventional protocols using broth culture and electroporation. (C) 2013 Elsevier B.V. All rights reserved.
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