Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 86, Issue 3, Pages 376-378Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2011.05.020
Keywords
Active bacteria; BrdU incorporation; Replicating bacteria; Soil bacteria; Thymidine analog
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Funding
- The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning
- Villum Kann Rasmussen Foundation
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Incorporation of 5-Bromo-2'-Deoxyuridine (BrdU) into DNA can be used to target replicating bacteria in the environment, but differential uptake capacity is a potential bias. Among 23 bacterial isolates commonly found in soils, most took up BrdU, but at up to 10-fold different cell-specific rates. Combined with results from an in silico analysis of 1000 BrdU-labeled 16S rRNA gene sequences, our results demonstrate a BrdU uptake bias with no apparent relationship between taxa affiliation and ability to incorporate BrdU. (C) 2011 Elsevier B.V. All rights reserved.
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