Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 76, Issue 3, Pages 316-319Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2008.12.003
Keywords
Real-time PCR; qPCR; Quantification; Mismatch; Genetic instability
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Funding
- Vienna Science and Technology Fund (WWTF), Austria [LS231]
- Austrian Christian Doppler Research Society
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Comparison of a broad range of characteristics of real-time PCR amplification curves yielded only slight alterations for low numbers of mismatches in the primer binding regions, resulting in a quantification error up to 63.12%. The effects were more pronounced for mismatches in the probe binding region and resulted in a quantification error up to 33%. (C) 2009 Elsevier B.V. All rights reserved.
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