Journal
JOURNAL OF MEMBRANE BIOLOGY
Volume 246, Issue 5, Pages 391-397Publisher
SPRINGER
DOI: 10.1007/s00232-013-9545-8
Keywords
Bisphenol A; Ca2+ channel; Protein kinase C; Protein kinase A; Dorsal root ganglion neuron
Funding
- National Natural Science Foundation of China [81072329]
- Innovation Project of Graduate Education of the Higher Education Institutions of Jiangsu Province, China [CX10B_348Z]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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The endocrine-disrupting chemical bisphenol A (BPA) is used to manufacture plastics including food containers, and it may leach into these containers. Consumption of BPA that has leached out of plastics may be harmful as recent research highlighted that BPA can induce alterations in the nervous system. In the present work, we studied the effects of BPA on Ca2+ channels in dorsal root ganglion (DRG) neurons. Using whole-cell patch-clamp recordings, we found that I (Ca) could be reduced by BPA in a concentration-dependent manner. Additionally, BPA shifted the activation curve of calcium currents toward a depolarizing direction and increased the slope factor of the curve. The inactivation curve for the currents was also assessed, and the curve shifted toward the depolarizing direction, although it was not significant. Moreover, inhibitory effects of BPA on the increments of intracellular Ca2+ concentrations ([Ca2+](i)) induced by 50 mM KCl were observed in DRG neurons using a laser scanning confocal microscopy assay. Further work revealed that the PKA and PKC pathways may be involved in the inhibitory effects of BPA since the PKA antagonist GA--6983 and the PKC antagonist H-89 significantly alleviated the inhibitory effects of BPA on I (Ca). As such, the results of the present study provide direct evidence that BPA decreases I (Ca) and impairs calcium homeostasis, which may be involved in any toxic effects of BPA on DRG neurons.
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