Journal
JOURNAL OF MEDICAL VIROLOGY
Volume 85, Issue 2, Pages 219-227Publisher
WILEY
DOI: 10.1002/jmv.23461
Keywords
hepatitis B virus; hepatitis B surface antigen; covalently closed circular DNA; total HBV DNA; hepatocellular carcinoma; chronic hepatitis B
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Funding
- Major Science and Technology Special Project of China Twelfth Five-year Plan [2013ZX10002004, 2012ZX10002003]
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The aim of the study was to investigate correlations between intrahepatic hepatitis B virus total DNA, covalently closed circular DNA (cccDNA), and serum HBsAg in treatment-naive chronic hepatitis B and HBV related hepatocellular carcinoma (HCC). Liver tissues were taken from 42 HBV related HCC and 36 patients with chronic hepatitis B. A fraction of DNA extracted from liver tissue was digested with a plasmid-safe ATP-dependent DNase and used for HBV cccDNA detection. The remaining DNA was used for the detection of HBV total DNA and beta-globin, the latter of which is a housekeeping gene and quantified for normalization by real-time PCR. Quantitation of serum HBsAg was performed by a chemiluminescence assay. Serum HBsAg had positive correlations with serum HBV DNA (r?=?0.636, P?
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