Journal
JOURNAL OF MEDICAL VIROLOGY
Volume 84, Issue 8, Pages 1267-1273Publisher
WILEY
DOI: 10.1002/jmv.23335
Keywords
parvoviruses; human bocavirus; persistence; PCR; EIA
Categories
Funding
- Finnish Academy [1122539]
- Sigrid Juselius Foundation
- Jenny and Antti Wihuri Foundation
- Medical Society of Finland (FLS)
- Eemil Aaltonen Foundation
- Maud Kuistila Foundation
- Foundation of Orion-Farmos
- Paulo Foundation
- Helsinki Biomedicum Foundation
- Helsinki University Central Hospital Research and Education Fund
- Research Funds of the University of Helsinki
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Human bocaviruses 1-4 (HBoV1-4) and parvovirus 4 (PARV4) are recently discovered human parvoviruses. HBoV1 is associated with respiratory infections of young children, while HBoV2-4 are enteric viruses. The clinical manifestations of PARV4 remain unknown. The objective of this study was to determine whether the DNAs of HBoV1-4 and PARV4 persist in human tissues long after primary infection. Biopsies of tonsillar tissue, skin, and synovia were examined for HBoV1-4 DNA and PARV4 DNA by PCR. Serum samples from the tissue donors were assayed for HBoV1 and PARV4 IgG and IgM antibodies. To obtain species-specific seroprevalences for HBoV1 and for HBoV2/3 combined, the sera were analyzed after virus-like particle (VLP) competition. While HBoV1 DNA was detected exclusively in the tonsillar tissues of 16/438 individuals (3.7%), all of them =8 years of age. HBoV2-4 and PARV4 DNAs were absent from all tissue types. HBoV1 IgG seroprevalence was 94.9%. No subject had HBoV1 or PARV4 IgM, nor did they have PARV4 IgG. The results indicate that HBoV1 DNA occurred in a small proportion of tonsils of young children after recent primary HBoV1 infection, but did not persist long in the other tissue types studied, unlike parvovirus B19 DNA. The results obtained by the PARV4 assays are in line with previous results on PARV4 epidemiology. J. Med. Virol. 84: 12671273, 2012. (C) 2012 Wiley Periodicals, Inc.
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