Review
Immunology
Jean-Claude Weill, Sandra Weller, Claude-Agnes Reynaud
Summary: Several species generate their preimmune repertoire in gut-associated lymphoid tissues (GALT) and diversify it through gene conversion and/or somatic hypermutation, enabling immune responses against systemic antigens. Similar processes occur in T-independent B cell responses in humans.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Eugenia Rita Lauriano, Alessio Alesci, Marialuisa Aragona, Simona Pergolizzi, Anthea Miller, Kristina Zuwala, Michal Kuciel, Giacomo Zaccone, Antonino Germana, Maria Cristina Guerrera
Summary: This study characterizes the leukocytes present in the gut-associated lymphoid tissue (GALT) of H. niloticus for the first time using confocal immuno-fluorescence techniques. The results show massive aggregates of immune cells in the submucosal thickness, forming oval-shaped structures similar to isolated lymphoid follicles found in birds and mammals. This expands our understanding of intestinal immunity in this fish.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sonia Moretti, Ivan Schietroma, Giuseppe Sberna, Maria Teresa Maggiorella, Leonardo Sernicola, Stefania Farcomeni, Marta Giovanetti, Massimo Ciccozzi, Alessandra Borsetti
Summary: HIV-1 replication in the gastrointestinal tract causes CD4+ T-cell depletion and disruption of the intestinal mucosa, leading to microbial translocation, inflammation, and immune activation. The gut serves as a viral reservoir with higher levels of HIV DNA compared to the blood. The interaction between HIV and key subsets of CD4+ T cells in the gut, as well as the role of gut microbiome and its metabolites in HIV-associated inflammation and immune activation, are important in the pathogenesis of HIV infection and related comorbidities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Eirini Moysi, Perla M. Del Rio Estrada, Fernanda Torres-Ruiz, Gustavo Reyes-Teran, Richard A. Koup, Constantinos Petrovas
Summary: CD4 T cells play a key role in adaptive immune responses during infections and vaccinations, with Tfh cells providing critical help to B-cells and contributing to HIV reservoir maintenance. Understanding the tissue-specific microenvironment and immune cell subsets that affect Tfh cell differentiation is important for designing effective prevention and cure strategies. The application of multispectral confocal microscopy and immunofluorescence panels offers a comprehensive approach for analyzing immune cell targets in lymphoid tissues.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Jing Chen, Tong Zhou, Yuan Zhang, Shumin Luo, Huan Chen, Dexi Chen, Chuanyun Li, Weihua Li
Summary: The persistence of latent HIV reservoir is a major challenge in curing HIV infection. Current antiretroviral therapy is unable to eliminate the latent HIV, hindering the progress of HIV treatment. This review aims to provide a comprehensive understanding of the existence, formation, localization, detection, and removal methods of latent HIV reservoir, in order to assist future research and potential role in achieving HIV treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Peter B. Jorgensen, Thomas M. Fenton, Urs M. Morbe, Lene B. Riis, Henrik L. Jakobsen, Ole H. Nielsen, William W. Agace
Summary: The authors present a protocol for isolating and studying human gut-associated lymphoid tissue, which allows identification and isolation of all human GALT and GALT-free intestinal lamina propria. This technique involves mechanical separation of intestinal mucosa from the submucosa to identify and isolate specific types of lymphoid tissue for downstream cellular and molecular characterization. The protocol, taking 4-10 hours, could be useful for researchers interested in intestinal immune development and function in health and disease.
Article
Immunology
Lesley R. de Armas, Christina Gavegnano, Suresh Pallikkuth, Stefano Rinaldi, Li Pan, Emilie Battivelli, Eric Verdin, Ramzi T. Younis, Rajendra Pahwa, Sion L. Williams, Raymond F. Schinazi, Savita Pahwa
Summary: The study used the dual reporter virus HIVGKO to investigate latency establishment and maintenance in CD4(+) T cells, and analyzed latently infected cells using single cell technologies. The research found that JAK1/2 inhibitors could reduce HIV infection events and block HIV reactivation from latent cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Patricia Recordon-Pinson, Annie Gosselin, Petronela Ancuta, Jean-Pierre Routy, Herve Fleury
Summary: The study compared the HIV-1 proviral DNA sequences in blood and gut-associated lymphoid tissue (GALT), finding them to be similar and suggesting that the epitope analysis of HIV-1 provirus in blood is relevant to that observed in the GALT. This indicates that blood data can be used for therapeutic vaccine approaches targeting HIV-1.
Article
Microbiology
Alessandra Borgognone, Marc Noguera-Julian, Bruna Oriol, Laura Noel-Romas, Marta Ruiz-Riol, Yolanda Guillen, Mariona Parera, Maria Casadella, Clara Duran, Maria C. Puertas, Francesc Catala-Moll, Marlon De Leon, Samantha Knodel, Kenzie Birse, Christian Manzardo, Jose M. Miro, Bonaventura Clotet, Javier Martinez-Picado, Jose Molto, Beatriz Mothe, Adam Burgener, Christian Brander, Roger Paredes
Summary: The study finds that the Bacteroidales/Clostridiales ratio is associated with immune control and the size of the HIV-1 reservoir after ART interruption. The gut microbiome of controllers is enriched with pro-inflammatory bacteria and depleted in butyrate-producing bacteria and methanogenic archaea. Additionally, functional analysis shows that metabolic pathways related to fatty acid and lipid biosynthesis are increased in controllers.
Review
Immunology
Toshiaki Kogame, Kenji Kabashima, Gyohei Egawa
Summary: The article discusses the evolution and function of the acquired immune tissue MALT, and introduces the research on the inducible form iSALT of the skin-associated lymphoid tissue SALT in a mouse model. The study shows that iSALT may play an important role in various skin diseases, with similar biological significance to MALT.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Alf S. Dalum, Aurora Kraus, Shanawaz Khan, Erna Davydova, Dimitri Rigaudeau, Havard Bjorgen, Adrian Lopez-Porras, Gareth Griffiths, Geert F. Wiegertjes, Erling O. Koppang, Irene Salinas, Pierre Boudinot, Julien Resseguier
Summary: Recent research has revealed the structured organization of gill-associated lymphoid tissue (GIALT) in adult zebrafish, with at least five distinct sub-regions identified. The GIALT in zebrafish is diffuse in some areas, such as the pharyngeal part of the gill arch, interbranchial septum, and filaments/lamellae, while it is structured in others, including the interbranchial lymphoid tissue (ILT) and a newly discovered Amphibranchial Lymphoid Tissue (ALT) located along each side of the gill arch.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Geriatrics & Gerontology
Yuchen Li, Xia Li, Qian Xue, Jingtong Wang, Jiang Tan
Summary: Recent studies have linked obesity with systemic T cell senescence and thymus involution, but the influence of high-fat diet (HFD) and diet composition on premature T cell senescence remains unclear. This study showed that HFD components synergistically induce T cell senescence in the gut-associated lymphoid tissue (GALT) with dyslipidemia. The results demonstrated a shift from naive to effector-memory (EM) phenotype in T cells and up-regulation of PD1 and KLRG1 in DIO mice, highlighting the complex interplay of HFD, obesity, and intestinal immunity.
EXPERIMENTAL GERONTOLOGY
(2021)
Review
Immunology
Urs M. Morbe, Peter B. Jorgensen, Thomas M. Fenton, Nicole von Burg, Lene B. Riis, Jo Spencer, William W. Agace
Summary: Human GALT, including Peyer's patches, appendix, and ILF, plays a key role in antigen sampling and adaptive immune responses in the intestinal wall. Current understanding of human GALT is primarily based on studies in mice, and its relevance to human health remains unclear. Further research is needed to explore the structure, composition, and potential regulatory functions of human GALT in intestinal immune responses.
MUCOSAL IMMUNOLOGY
(2021)
Article
Immunology
D. Porter, David Peggs, C. McGurk, Samuel A. M. Martin
Summary: This study aimed to evaluate the suitability of primary gut-associated lymphoid tissue (GALT) cells and rainbow trout cell lines as alternative methods for testing functional feed ingredients. The results showed that GALT cells were the most effective for health screening, while rainbow trout cell lines exhibited a weaker T-cell response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Ji Taek Hong, Eun Ran Kim, Sung-Wook Park, Ji Won Kim, Sung Noh Hong, Dong Kyung Chang, Young-Ho Kim, Jun Hun Cho
Summary: The majority of rectal LLH cases resolve spontaneously, but about 10% may develop into serious diseases such as UC or MALT lymphoma. Patients with nodular or submucosal tumor-like types may have a higher risk of developing clinically significant diseases. Risk stratification and careful surveillance are essential for these lesions.
Article
Immunology
Jade Ghosn, Lambert Assoumou, Caroline Lascoux-Combe, Gilles Peytavin, Karine Amat, Audrey Gabassi, Minh P. Le, Robert Nzalakanda, Nadia Valin, Roland Landman, Marie-Laure Chaix, Constance Delaugerre
Summary: In men starting dolutegravir-based regimen at primary HIV infection, achieving first undetectable HIV-1 RNA in seminal plasma was significantly faster compared to blood plasma, with a higher proportion reaching undetectable levels in seminal plasma.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Biochemical Research Methods
Assaf Mizrahi, Jean-Claude Nguyen Van, Najoua El Helali, Julie Lourtet-Hascoet, Ines Jabnoune, Marie Liesse Pacreau, Yasmina Talb, Jacques Fourgeaud, Marianne Leruez-Ville, Benoit Pilmis, Veronique Avettand-Fenoel, Alban Le Monnier
Summary: This study described the implementation of the Coris BioConcept COVID-19 Ag Respi-Strip test in a clinical microbiology laboratory for COVID-19 diagnosis, with performance evaluation against a RT-PCR kit as the gold standard. The 30-minute readings slightly improved the sensitivity compared to the 15-minute readings, but the overall sensitivity was still relatively low.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Multidisciplinary Sciences
Chris Wymant, Daniela Bezemer, Francois Blanquart, Luca Ferretti, Astrid Gall, Matthew Hall, Tanya Golubchik, Margreet Bakker, Swee Hoe Ong, Lele Zhao, David Bonsall, Mariateresa de Cesare, George MacIntyre-Cockett, Lucie Abeler-Doerner, Jan Albert, Norbert Bannert, Jacques Fellay, M. Kate Grabowski, Barbara Gunsenheimer-Bartmeyer, Huldrych F. Gunthard, Pia Kivela, Roger D. Kouyos, Oliver Laeyendecker, Laurence Meyer, Kholoud Porter, Matti Ristola, Ard van Sighem, Ben Berkhout, Paul Kellam, Marion Cornelissen, Peter Reiss, Christophe Fraser
Summary: A highly virulent variant of subtype-B HIV-1 was discovered in the Netherlands. Infected individuals with this variant had significantly higher viral loads and faster decline in CD4 cells compared to other subtype-B strains. The increased virulence is attributed to the viral strain, and the variant emerged in the 1990s with increased transmissibility and an unfamiliar molecular mechanism of virulence.
Article
Multidisciplinary Sciences
Mathieu Claireaux, Remy Robinot, Jerome Kervevan, Mandar Patgaonkar, Isabelle Staropoli, Anne Brelot, Alexandre Nouel, Stacy Gellenoncourt, Xian Tang, Melanie Hery, Stevenn Volant, Emeline Perthame, Veronique Avettand-Fenoel, Julian Buchrieser, Thomas Cokelaer, Christiane Bouchier, Laurence Ma, Faroudy Boufassa, Samia Hendou, Valentina Libri, Milena Hasan, David Zucman, Pierre de Truchis, Olivier Schwartz, Olivier Lambotte, Lisa A. Chakrabarti
Summary: Individuals who can naturally control HIV infection have lower levels of the viral co-receptor CCR5 in specific CD4 (+) T cells, which is due to mutations or receptor internalization. These individuals also maintain CD4 + T cells with high avidity for Gag antigens and potent effector functions. The downregulation of CCR5 in specific CD4 + T cells contributes to decreased susceptibility to CCR5-dependent HIV entry in these individuals.
NATURE COMMUNICATIONS
(2022)
Article
Infectious Diseases
Pierre Frange, Veronique Avettand-Fenoel, Florence Veber, Stephane Blanche
Summary: This study provides long-term data on changes in body mass index (BMI) in French children and adolescents receiving dolutegravir. The results show that there was no evidence of change in BMIz after initiating dolutegravir therapy for pediatric patients.
Article
Environmental Sciences
S. Wurtzer, P. Waldman, M. Levert, N. Cluzel, J. L. Almayrac, C. Charpentier, S. Masnada, M. Gillon-Ritz, J. M. Mouchel, Y. Maday, M. Boni, V. Marechal, L. Moulin, S. Le Guyader, I. Bertrand, C. Gantzer, Diane Descamps, Charlotte Charpentier, Nadhira Houhou, Benoit Visseaux, Vincent Calvez, Anne-Genevieve Marcelin, Stephane Marot, Aude Jary, Marie-Laure Chaix, Constance Delaugerre, Maud Salmona, Jerome Legoff, Elyanne Gault, Marie-Anne Rameix-Welti, Anne-Sophie L'honneur, Alice Andree Mariaggi, Flore Rozenberg, Anne-Marie Roque, Lina Mouna, Honorine Fenaux, Marianne Leruez-Ville, Laurence Morand-Joubert, Sidonie Lambert-Niclot, Aurelie Schnuriger, Claire Dubois, Nadege Bourgeois-Nicolaos, Segolene Brichler, Heloise Delagreverie, Magali Bouvier-Alias, Slim Fourati, Christophe Rodriguez, Jean-Michel Pawlotsky
Summary: The study emphasizes the importance of detecting SARS-CoV-2 in wastewater for monitoring the COVID-19 pandemic. The correlation between wastewater monitoring indicators and health data, as well as the predominant role played by specific variants in outbreak dynamics, have been observed and highlighted.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Infectious Diseases
Tangui Barre, Patrick Mercie, Caroline Lions, Patrick Miailhes, David Zucman, Hugues Aumaitre, Laure Esterle, Philippe Sogni, Patrizia Carrieri, Dominique Salmon-Ceron, Fabienne Marcellin
Summary: According to a study, following HCV cure, reduction in cannabis use is associated with reduction in tobacco use, improved well-being, and adoption of healthy behaviors.
AIDS RESEARCH AND THERAPY
(2022)
Article
Medicine, General & Internal
Roxane Verdikt, Maryam Bendoumou, Sophie Bouchat, Lorena Nestola, Alexander O. Pasternak, Gilles Darcis, Veronique Avettand-Fenoel, Caroline Vanhulle, Amina Ait-Ammar, Marion Santangelo, Estelle Plant, Valentin Le Douce, Nadege Delacourt, Coca Necsoi, Francis Corazza, Caroline Pereira Bittencourt Passaes, Christian Schwartz, Martin Bizet, Francois Fuks, Asier Saez-Cirion, Christine Rouzioux, Stephane De Wit, Ben Berkhout, Virginie Gautier, Olivier Rohr, Carine Van Lint
Summary: This study uncovers specific demethylation CpG signatures induced by 5-AzadC in the HIV-1 promoter and reveals the recruitment of UHRF1 to the HIV-1 promoter. It demonstrates the role of UHRF1 in the epigenetic repression of the latent viral promoter.
Article
Immunology
Jeanne Sibiude, Jerome Le Chenadec, Laurent Mandelbrot, Alexandre Hoctin, Catherine Dollfus, Albert Faye, Eida Bui, Emmanuelle Pannier, Jade Ghosn, Valerie Garrait, Veronique Avettand-Fenoel, Pierre Frange, Josiane Warszawski, Roland Tubiana
Summary: Antiretroviral therapy initiated before pregnancy and continued throughout pregnancy can significantly reduce perinatal transmission of HIV. Factors such as not breastfeeding, infant prophylaxis, and maternal viral suppression near delivery play important roles in preventing transmission.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Microbiology
Pauline Tremeaux, Frederic Lemoine, Adeline Melard, Marine Gousset, Faroudy Boufassa, Sylvie Orr, Valerie Monceaux, Olivier Gascuel, Olivier Lambotte, Laurent Hocqueloux, Asier Saez-Cirion, Christine Rouzioux, Veronique Avettand-Fenoel
Summary: In the search for ways to control HIV-1 infection without antiretroviral therapy, posttreatment controllers (PTCs) are models of HIV remission. We characterized the HIV blood reservoirs of PTCs in comparison with other groups and found that PTCs had lower proviral diversity and smaller amounts of defective proviruses than CHIs. These findings suggest potential mechanisms of control in PTCs and highlight the importance of further research in this area. Rating: 7/10.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Laurent Mandelbrot, Roland Tubiana, Pierre Frange, Gilles Peytavin, Jerome Le Chenadec, Ana Canestri, Philippe Morlat, Cecile Brunet-Cartier, Jeanne Sibiude, Delphine Peretti, Veronique Chambrin, Amelie Chabrol, Eida Bul, Caroline Simon-Toulza, Lucie Marchand, Christelle Paul, Sandrine Delmas, Veronique Avettand-Fenoel, Josiane Warszawski, Monogest Study Grp
Summary: This study evaluated a perinatal NRTI-sparing strategy to prevent perinatal HIV transmission. The primary objective was to determine the proportion of patients maintaining a viral load (VL) of <50 copies/mL on darunavir/ritonavir monotherapy without needing treatment intensification. The results showed that nearly 10% of cases required treatment intensification, limiting the widespread use of darunavir/ritonavir maintenance monotherapy.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Virology
Marie-Laure Chaix, Nicolas Leturque, Audrey Gabassi, Isabelle Charreau, Marine Minier, Gilles Pialoux, Eric Cua, Christian Chidiac, Francois Raffi, Cecile Tremblay, Laurence Meyer, Jean-Michel Molina, Constance Delaugerre
Summary: The seroprevalence and incidence of hepatitis E virus (HEV) infection were investigated in 416 men who have sex with men (MSM). Older age was the only factor associated with testing seropositive for HEV. Factors such as geographical origin, use of recreational drugs, number of sexual partners, and other sexually transmitted infections were not associated. Among the HEV-seronegative patients, 9 individuals seroconverted during the follow-up period.
JOURNAL OF CLINICAL VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Helene M. Roux, Amira Marouf, Jacques Dutrieux, Benedicte Charmeteau-De Muylder, Suzanne Figueiredo-Morgado, Veronique Avettand-Fenoel, Pelagia Cuvelier, Cecile Naudin, Fatma Bouaziz, Guillaume Geri, Anne Couedel-Courteille, Pierre Squara, Stefano Marullo, Remi Cheynier
Summary: Thymic activation improves the outcome of COVID-19 patients with severe pneumonia. Genetic polymorphism that affects thymic output can modify SARS-CoV-2 immunity and disease severity. GG genotype is associated with stronger and long-lasting immune response and less severe lung involvement.
Article
Gastroenterology & Hepatology
Fabienne Marcellin, Sylvie Bregigeon-Ronot, Clemence Ramier, Camelia Protopopescu, Camille Gilbert, Vincent Di Beo, Claudine Duvivier, Morgane Bureau-Stoltmann, Eric Rosenthal, Linda Wittkop, Dominique Salmon-Ceron, Patrizia Carrieri, Philippe Sogni, Tangui Barre
Summary: This study aimed to investigate the prevalence of moderate-to-severe depression in people living with HIV and HCV after successful HCV treatment, and identify associated socio-behavioral factors. Through descriptive and logistic regression analyses of data from 398 participants, it was found that 23.9% of HCV-cured individuals had moderate-to-severe depression. Female sex, unhealthy alcohol use, sedentary lifestyle, and unhealthy eating behaviors were associated with increased odds of moderate-to-severe depression.
Article
Infectious Diseases
A. Maisa, G. Spaccaferri, L. Fournier, J. Schaeffer, J. Deniau, P. Rolland, B. Coignard
Summary: This study investigated the first cases of Omicron detected in France and found that most cases presented with mild symptoms, including young individuals and those who were vaccinated. The findings provide important support for the national COVID-19 surveillance system.
INFECTIOUS DISEASES NOW
(2022)