Review
Microbiology
Xintong Peng, Yanling Zhou, Yongguang Tao, Shuang Liu
Summary: This article summarizes the relationship between EBV, EGFR, and LMP1 in NPC, highlighting the importance of EGFR in the occurrence and development of NPC. EGFR-targeted drugs are discussed as potential newly developed anti-tumor drugs for NPC patients, as activation of EGFR signaling affects various aspects of NPC cell behavior and contributes to poor outcomes.
Article
Microbiology
Kina Kase, Satoru Kondo, Naohiro Wakisaka, Hirotomo Dochi, Harue Mizokami, Eiji Kobayashi, Makoto Kano, Takeshi Komori, Nobuyuki Hirai, Takayoshi Ueno, Yosuke Nakanishi, Miyako Hatano, Kazuhira Endo, Makiko Moriyama-Kita, Hisashi Sugimoto, Tomokazu Yoshizaki
Summary: The study found that LMP1 can induce the expression of sPD-L1 and PD-L1 in vitro and that the expression of LMP1 is significantly correlated with PD-L1 in NPC patients. Additionally, the serum level of sPD-L1 in NPC patients was higher than in controls, particularly in advanced stage patients. However, the expression of LMP1, PD-L1, and sPD-L1 levels were not associated with prognosis.
Article
Biotechnology & Applied Microbiology
Saidu Kamara, Yanru Guo, Shanshan Mao, Xiaoxian Ye, Qingfeng Li, Maolin Zheng, Jinshun Zhu, Jing Zhang, Wangqi Du, Jun Chen, Shanli Zhu, Lifang Zhang
Summary: Novel EBV LMP1 C-terminal domain affibody molecules were selected and demonstrated high affinity and specificity for EBV LMP1 protein. In tumor-bearing nude mice, these affibody molecules rapidly accumulated at the tumor site, indicating their potential as specific molecular imaging agents for EBV-associated NPC.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
San Xu, Zhuan Zhou, Xingzhi Peng, Xuxiu Tao, Peijun Zhou, Kun Zhang, Jinwu Peng, Dan Li, Liangfang Shen, Lifang Yang
Summary: Studies have shown that LMP1 enhances autophagy by upregulating BNIP3 expression, leading to increased radioresistance in nasopharyngeal carcinoma cells. Through the ERK/HIF1α signaling axis, LMP1 promotes the binding of BNIP3 to Beclin1 and competitively reduces the binding of Bcl-2 to Beclin1, thereby upregulating autophagy. Knockdown of BNIP3 reduces radioresistance mediated by protective autophagy, highlighting its crucial role in LMP1-positive nasopharyngeal carcinoma cells' protection against irradiation.
CELL DEATH & DISEASE
(2021)
Review
Oncology
Xishan Chen, Renba Liang, Lin Lai, Kaihua Chen, Xiaodong Zhu
Summary: The meta-analysis showed that high expression of EGFR was significantly associated with poor overall survival (OS) and disease-free survival (DFS) in patients with nasopharyngeal carcinoma. However, it was not significantly associated with other survival indicators. High expression of p-EGFR did not show significant correlation with OS and distant metastasis-free survival (DMFS).
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Haiwen Chen, Xiaobing Duan, Xiaohong Deng, Yingping Huang, Xiang Zhou, Shanshan Zhang, Xiao Zhang, Pingjuan Liu, Chaopin Yang, Guojun Liu, Qinqin Ren, Yan Xiong, Bo Zhu, Jiexia Zhang, Tong Xiang
Summary: Nasopharyngeal carcinoma is an EBV-associated epithelial malignancy characterized by infiltration of lymphocytes, including NK cells. EBV-positive NPC cells often develop mechanisms to evade NK cell immune surveillance. Understanding the mechanisms involved in EBV-induced NK cell dysfunction may lead to the development of novel immunotherapies for NPC.
CANCER IMMUNOLOGY RESEARCH
(2023)
Article
Immunology
Yanru Guo, Saidu Kamara, Jing Zhang, He Wen, Maolin Zheng, Ying Liu, Luqi Zhou, Jun Chen, Shanli Zhu, Lifang Zhang
Summary: Three potential Z(LMP1-C) affibody molecules (Z(LMP1-C)15, Z(LMP1-C)114 and Z(LMP1-C)277) were found to have specific binding interactions with recombinant and native EBV LMP1, showing high antitumor effects on EBV-positive NPC cell lines without cytotoxicity towards EBV-negative NPC cell line. Among them, Z(LMP1-C)277 exhibited the highest antitumor efficacy and could decrease the phosphorylation levels of up-stream activators, leading to downstream suppression of phospho-p90RSK and c-Fos. Treatment with Z(LMP1-C)277 reduced tumor growth in mice without apparent toxicity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Saidu Kamara, Yanru Guo, He Wen, Ying Liu, Lei Liu, Maolin Zheng, Jing Zhang, Luqi Zhou, Jun Chen, Shanli Zhu, Lifang Zhang
Summary: Antibodies are important therapeutic agents in cancer treatment, with bispecific antibodies (BsAbs) being a next-generation strategy. However, their large size leads to poor tumor penetration. On the other hand, affibody molecules have shown promise in molecular imaging diagnostics and targeted tumor therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Ana Banko, Danijela Miljanovic, Ivana Lazarevic, Andja Cirkovic
Summary: This study investigated the connection between LMP1 gene variability and NPC development, finding an association between 30bp deletion and Xhol loss with NPC susceptibility, but 30bp deletion could not distinguish NPC from EBV-associated non-NPC tumors. Certain EBV variants were rare in NPC individuals, signaling the need for further research to verify the biological significance of these differences.
Article
Virology
Nannan Zhu, Qian Wang, Zhidong Wu, Yan Wang, Mu-Sheng Zeng, Yan Yuan
Summary: Epstein-Barr virus (EBV) is associated with various malignant diseases, including nasopharyngeal carcinoma (NPC). The virus-encoded oncoprotein LMP1 promotes the development of cancer stem cells (CSCs) in NPC by activating the PI3K/mTOR/Akt signaling pathway. Different mTOR pathways, mTORC1 and mTORC2, contribute to the acquisition of distinct CSC characteristics and cooperate to regulate NPC metastasis.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Nannan Zhu, Xiaoting Xu, Yan Wang, Musheng Zeng, Yan Yuan
Summary: The coordinated action of EBV membrane proteins LMP1 and LMP2A generates various intermediate subpopulations in the EMT spectrum in nasopharyngeal carcinoma, which are responsible for distinct tumorigenic features such as tumor initiation, vasculogenesis, and metastasis.
Review
Medicine, General & Internal
Xiaohua Hong, Guangyao Wang, Guanglan Xu, Wei Shi, Tongbiao Wang, Zhen Rong, Chunmei Mo
Summary: The article evaluated the prognostic effect and clinical significance of epidermal growth factor receptor and its phosphorylated form (EGFR/p-EGFR) in nasopharyngeal carcinoma through a systematic review and meta-analysis. The results showed that EGFR overexpression could be used as a biomarker for poor overall survival (OS) and disease-free survival (DFS) in nasopharyngeal carcinoma, but not for progression-free survival (PFS) and distant metastasis-free survival (DMFS). The overexpression of p-EGFR was not associated with the prognosis of nasopharyngeal carcinoma patients.
Article
Biology
Qi Yuan, Bing-Hong Chen, Dai-jia Huang, Rong Zhang
Summary: RNA interference (RNAi) treatment is an important therapeutic approach in cancer by downregulating target-oncogenes, but its clinical efficacy still requires further investigation. The mechanism of LMP1 as a proto-oncogene in nasopharyngeal carcinoma is still unclear.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2023)
Article
Oncology
Renba Liang, Xiaodong Zhu
Summary: UC2288 inhibited the growth of NPC by suppressing the EGFR/ERK pathway and may be a promising therapeutic option for NPC.
Article
Biotechnology & Applied Microbiology
Chenjing Ma, Xian Li, Weifeng Ding, Xin Zhang, Hang Chen, Ying Feng
Summary: In this study, the effects of introducing exogenous hTERT on Periplaneta americana cells were investigated. It was found that hTERT expression promoted cell growth and extended cell lifespan by increasing telomerase activity and elongating telomeres. This suggests that introducing hTERT into insect cells could be an efficient method to establish certain insect cell lines.
Article
Oncology
Josephine Mun-Yee Ko, Kay Hiu-Ki Tsang, Wei Dai, Sheyne Sta Ana Choi, Merrin Man-Long Leong, Roger Kai-Cheong Ngan, Dora Lai-Wan Kwong, Ashley Cheng, Anne Wing-Mui Lee, Wai Tong Ng, Stewart Tung, Victor Ho-Fun Lee, Ka-On Lam, Candy King-Chi Chan, Maria Li Lung
INTERNATIONAL JOURNAL OF CANCER
(2018)
Article
Oncology
Chen Jiang, Jinna Chen, Shanghang Xie, Lifang Zhang, Yanqun Xiang, Maria Lung, Ngar-Woon Kam, Dora Lai-wan Kwong, Sumei Cao, Xin-Yuan Guan
INTERNATIONAL JOURNAL OF CANCER
(2018)
Article
Oncology
Wai-Tong Ng, Roger K. C. Ngan, Dora L. W. Kwong, Stewart Y. Tung, Kam-Tong Yuen, Michael K. M. Kam, Henry C. K. Sze, Harry H. Y. Yiu, Lucy L. K. Chan, Maria L. Lung, Anne W. M. Lee
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2018)
Article
Medicine, Research & Experimental
Yim Ling Yip, Weitao Lin, Wen Deng, Lin Jia, Kwok Wai Lo, Pierre Busson, Benjamin Verillaud, Xuefeng Liu, Chi Man Tsang, Maria Li Lung, Sai Wah Tsao
LABORATORY INVESTIGATION
(2018)
Article
Multidisciplinary Sciences
Zhi-Ming Mai, Jia-Huang Lin, Shing-Chun Chiang, Roger Kai-Cheong Ngan, Dora Lai-Wan Kwong, Wai-Tong Ng, Alice Wan-Ying Ng, Kam-Tong Yuen, Kai-Ming Ip, Yap-Hang Chan, Anne Wing-Mui Lee, Sai-Yin Ho, Maria Li Lung, Tai-Hing Lam
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Merrin Man Long Leong, Arthur Kwok Leung Cheung, Wei Dai, Sai Wah Tsao, Chi Man Tsang, Christopher W. Dawson, Josephine Mun Yee Ko, Maria Li Lung
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Oncology
Josephine Mun Yee Ko, Hoi Yan Ng, Ka On Lam, Keith Wan Hang Chiu, Dora Lai Wan Kwong, Anthony Wing Ip Lo, Jean Chrysei Wong, Roger Chia Wei Lin, Henry Chun Hung Fong, Jason Ying Ki Li, Wei Dai, Simon Law, Maria Li Lung
Article
Biochemistry & Molecular Biology
Lai-Sheung Chan, Hong-Lok Lung, Roger Kai-Cheong Ngan, Anne Wing-Mui Lee, Sai Wah Tsao, Kwok-Wai Lo, Michael Kahn, Maria Li Lung, Rotraud Wieser, Nai-Ki Mak
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Oncology
Mingdan Deng, Wei Dai, Valen Zhuoyou Yu, Lihua Tao, Maria Li Lung
Article
Oncology
Wei Dai, Dittman Lai-Shun Chung, Larry Ka-Yue Chow, Valen Zhuoyou Yu, Lisa Chan Lei, Merrin Man-Long Leong, Candy King-Chi Chan, Josephine Mun-Yee Ko, Maria Li Lung
CLINICAL CANCER RESEARCH
(2020)
Article
Oncology
Lisa Chan Lei, Valen Zhuoyou Yu, Josephine Mun Yee Ko, Lvwen Ning, Maria Li Lung
Article
Oncology
Josephine Mun Yee Ko, Shiu Yeung Lam, Lvwen Ning, Annie Wai Yeeng Chai, Lisa Chan Lei, Sheyne Sta Ana Choi, Carissa Wing Yan Wong, Maria Li Lung
Summary: Unbiased whole-exome sequencing identified RAD50 as a potential cancer predisposition gene in familial esophageal squamous cell carcinoma (ESCC). Two pathogenic RAD50 protein truncation variants were found to significantly increase familial ESCC risk, with functional characterization suggesting a dominant-negative effect on DNA repair and sensitization to cell death. The study provides novel insights into potential therapeutic options for RAD50 variants and CHK1 inhibitors in ESCC.
Article
Oncology
Jian Li, Josephine Mun-Yee Ko, Wei Dai, Valen Zhuoyou Yu, Hoi Yan Ng, Jean-Sebastien Hoffmann, Maria Li Lung
Summary: High expression of POLQ in esophageal squamous cell carcinoma is associated with unfavorable prognosis, contributing to malignant phenotypes through promoting genome stability, suggesting it as a potential therapeutic target.
Article
Oncology
Josephine Mun Yee Ko, Ka On Lam, Dora Lai Wan Kwong, Ian Yu-Hong Wong, Fion Siu-Yin Chan, Claudia Lai-Yin Wong, Kwan Kit Chan, Tsz Ting Law, Keith Wan Hang Chiu, Candy Chi Shan Lam, Jean Chrysei Wong, Henry Chun Hung Fong, Faith Sin Fai Choy, Andy Lo, Simon Law, Maria Li Lung
Summary: Esophageal cancer is a aggressive disease with low survival rates. Circulating tumor cells (CTCs) can be used as predictive and prognostic biomarkers for therapeutic responses and disease progression. Longitudinal CTC monitoring can provide useful information for prediction of treatment efficacies and disease progression, and it can be used as a non-invasive liquid biopsy for supplementary information with clinical imaging.
Article
Oncology
Luo Chen, Wai Yin Chau, Hei Tung Yuen, Xiao Han Liu, Robert Zhong Qi, Maria Li Lung, Hong Lok Lung
Summary: This study showed that THY1 suppresses tumor invasion in NPC through the formation of adherens junctions and inhibition of SRC and canonical Wnt signaling. PTPN22 was identified as a key factor in THY1-mediated inhibition of cell invasion and SRC activity. PDGF-Rb and SRC were identified as potential drug targets for suppressing NPC metastasis.