Article
Biology
Iris Wagner, Linda Grigoraki, Peter Enevoldson, Michael Clarkson, Sam Jones, Jane L. L. Hurst, Robert J. J. Beynon, Hilary Ranson
Summary: The rapid evaporative ionisation mass spectrometry (REIMS) method can accurately identify the species and age of mosquitoes, with high accuracy in both laboratory-reared and wild populations.
Article
Chemistry, Analytical
Samira Beyramysoltan, Megan I. Chambers, Amy M. Osborne, Monica I. Ventura, Rabi A. Musah
Summary: The misuse of legal high psychoactive plants is a global concern due to the negative impacts on public health and safety. However, the development of methods to rapidly identify these substances is a major challenge. In this study, a user-friendly tool called Database of Psychoactive Plants (DoPP) was developed for the identification of unknown plants and quantification of psychoactive compounds. The tool utilizes real-time high-resolution mass spectrometry analysis to reveal species-specific chemical signatures of terrestrial plants, allowing for rapid discrimination and species identification.
ANALYTICAL CHEMISTRY
(2022)
Article
Optics
Geon Kim, Daewoong Ahn, Minhee Kang, Jinho Park, DongHun Ryu, YoungJu Jo, Jinyeop Song, Jea Sung Ryu, Gunho Choi, Hyun Jung Chung, Kyuseok Kim, Doo Ryeon Chung, In Young Yoo, Hee Jae Huh, Hyun-seok Min, Nam Yong Lee, YongKeun Park
Summary: The healthcare industry urgently needs rapid microbial identification techniques for treating microbial infections. This study proposes a microscopy-based framework that can identify pathogens from single cells or clusters, providing an effective tool for clinical applications.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Agriculture, Multidisciplinary
Teng Yang, Zhen Li, Jinian Shu, Longfa Fang, Xiaojuan He, Qirun Li, Guoxia Jiang, Siwei Chen, Bo Yang
Summary: This study identified potential natural food preservatives using single-photon ionization time-of-flight mass spectrometry (SPI-TOF-MS) from herbs. The random forest algorithm was used to distinguish different Artemisia species by identifying characteristic peaks of volatile terpenoids (VTPs). The study revealed an expanded terpenoid synthase gene family in Artemisia species, which potentially contributes to increased production of VTPs, serving as natural preservatives and specifically identifying these species. The use of SPI-TOF-MS allowed for low detection limits (22-39 pptv) of principal VTPs in Artemisia species. This study highlights the potential of headspace mass spectrometry in developing natural preservatives and identifying plant species.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Marianna Manninen, Maarit Karonen, Juha-Pekka Salminen
Summary: In this study, a chemotaxonomic tool based on UHPLC-MS was developed to identify common Finnish deciduous trees and shrubs from their leaf bud metabolites. The study found that various plant metabolites can serve as good chemotaxonomic markers, with species-specific markers being superior in creating fingerprints.
Article
Environmental Sciences
Alina T. Roman-Hubers, Thomas J. McDonald, Erin S. Baker, Weihsueh A. Chiu, Ivan Rusyn
Summary: This study examined the potential of IMS-MS as a high-throughput method for the chemical characterization of crude oils, finding it to be either equal or better than GC-MS in classifying the origins of crude oils and greatly increasing sample analysis throughput. The study demonstrated the utility of IMS-MS for rapid fingerprinting of complex samples and showed its advantages over traditional GC-MS-based analyses in emergency decision-making situations.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2021)
Article
Chemistry, Analytical
Haiyan Gao, Jihong Lin, Xiaofei Jia, Yang Zhao, Songying Wang, Hua Bai, Qiang Ma
Summary: In this study, a simplified workflow utilizing REIMS and an electric soldering iron was developed for authenticating leather samples. A chemometric model based on authentic leather samples was created to classify commercial leather samples in real-time, with scanning electron microscopy used to characterize leather microstructures. This study is expected to provide a high-throughput tool for authenticating leather and other biogenic consumer products with superior efficiency and accuracy.
Article
Chemistry, Analytical
Jonathan Martens, Rianne E. van Outersterp, Jitse Oosterhout, Christoph R. Gebhardt, Giel Berden, Udo F. H. Engelke, Ron A. Wevers, Filip Cuyckens, Jos Oomens
Summary: Infrared ion spectroscopy (IRIS) is a useful tool for identifying molecular structures detected in mass spectrometry (MS) experiments. However, coupling LC and IRIS is challenging due to the different timescales. To address this, we propose a heartcutting LC-IRIS approach using two switching valves and two sample loops as an alternative to direct coupling.
ANALYTICAL CHEMISTRY
(2023)
Article
Forestry
Dominika Szadkowska, Janusz Zawadzki, Pawel Kozakiewicz, Andrzej Radomski
Summary: The chemical composition of extractives in five species of poplar wood was investigated, with a focus on their potential as raw material for biofuel manufacturing. Identification of the extractives is challenging due to the variety of compounds and the large number of individual ones present. Gas chromatography coupled with mass spectrometer was used for the analysis of extractives.
Article
Microbiology
Simon Robinne, Jamal Saad, Madjid Morsli, Zelika Harouna Hamidou, Fatah Tazerart, Michel Drancourt, Sophie Alexandra Baron
Summary: This study reports a method using MALDI-TOF-MS technology to discriminate Mycobacterium tuberculosis complex mycobacteria, which can be easily implemented in clinical microbiology laboratories.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
T. Garrigos, C. Neuwirth, A. Chapuis, J. Bador, L. Amoureux
Summary: The study aimed to establish a MALDI-TOF/MS Bruker database including 18 Achromobacter species and evaluate its suitability in laboratory settings. The new database accurately identified 99.4% of isolates, significantly outperforming the Bruker default database at 50.9%. This highlights the potential of using MALDI-TOF/MS with a local database for rapid and accurate identification.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Chemistry, Applied
Seong Yeon Choi, Birang Jeong, Eunyoung Mok, Yongsoo Kwon, Heejung Yang
Summary: Ion-fragmentation information from HRMS is used as chemometric data for analyzing molecular distribution and changes in food-ingredient metabolites. In this study, molecular networking (MN) methods combined with in silico annotation methods and multivariate analysis were used to identify six discriminative markers for four Citrus samples. These markers were experimentally verified and quantitatively monitored based on their species and maturity.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Gastroenterology & Hepatology
Josef Ecker, Elisa Benedetti, Alida S. D. Kindt, Marcus Hoering, Markus Perl, Andrea Christel Machmueller, Anna Sichler, Johannes Plagge, Yuting Wang, Sebastian Zeissig, Andrej Shevchenko, Ralph Burkhardt, Jan Krumsiek, Gerhard Liebisch, Klaus-Peter Janssen
Summary: Analysis of the CRC lipidome revealed a robust TG-species signature with prognostic potential, shedding light on the importance of lipid metabolism abnormalities in CRC.
Article
Chemistry, Analytical
Rianne E. van Outersterp, Udo F. H. Engelke, Jona Merx, Giel Berden, Mathias Paul, Thomas Thomulka, Albrecht Berkessel, Marleen C. D. G. Huigen, Leo A. J. Kluijtmans, Jasmin Mecinovic, Floris P. J. T. Rutjes, Clara D. M. van Karnebeek, Ron A. Wevers, Thomas J. Boltje, Karlien L. M. Coene, Jonathan Martens, Jos Oomens
Summary: This study utilized untargeted metabolomics strategies and infrared ion spectroscopy to identify novel biomarkers for pyridoxine-dependent epilepsy, providing feasible diagnostic analysis for newborn screening.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Swati Baskiyar, Chang Ren, Kabre L. Heck, Audrey M. Hall, Muhammad Gulfam, Sadaira Packer, Cheryl D. Seals, Angela I. Calderon
Summary: This study presents an automated workflow combining multiple computational tools to quickly create bioactive molecular networks, greatly improving the efficiency of discovering bioactive compounds from natural products.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Virology
Philippe Colson, Philippe Gautret, Jeremy Delerce, Herve Chaudet, Pierre Pontarotti, Patrick Forterre, Raphael Tola, Marielle Bedotto, Lea Delorme, Wahiba Bader, Anthony Levasseur, Jean-Christophe Lagier, Matthieu Million, Nouara Yahi, Jacques Fantini, Bernard La Scola, Pierre-Edouard Fournier, Didier Raoult
Summary: This study investigated the dynamics of the French variant Marseille-4 of SARS-CoV-2 over a 10-month period, identifying two subvariants and analyzing their mutations and lifetimes. The findings suggest that beneficial mutations can initiate new outbreaks, while the accumulation of other mutations can result in the disappearance of lineages.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Microbiology
Reham Magdy Wasfy, Malak Zoaiter, Melhem Bilen, Maryam Tidjani Alou, Cheikh Ibrahima Lo, Sara Bellali, Aurelia Caputo, Stephane Alibar, Claudia Andrieu, Didier Raoult, Pierre Edouard Fournier, Matthieu Million
Summary: This article reports a novel bacterial strain Marseille-P4005(T) isolated from a stool sample of a healthy donor. It is a Gram-stain negative, non-motile, non-spore-forming rod that grows optimally at 37 degrees C and at pH 7.0. Genomic analysis revealed it as a new species within the genus Agathobaculum, named Agathobaculum massiliense sp. nov. The strain showed resistance to several antibiotics and exhibited specific metabolic capabilities.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2023)
Review
Infectious Diseases
Myrto Koutantou, Konstantinos Kambas, Sofia Makka, Pierre-Edouard Fournier, Didier Raoult, Emmanouil Angelakis
Summary: Cat scratch disease (CSD) is difficult to diagnose, and serological assays have limitations in terms of sensitivity and specificity. Molecular assays should be considered as the gold standard for confirming CSD, and lymph node biopsy may be necessary in suspicious cases.
CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY
(2023)
Article
Microbiology
Sory Ibrahima Traore, Cheikh Ibrahima Lo, Maaloum Mossaab, Guillaume Durand, Jean Christophe Lagier, Didier Raoult, Pierre-Edouard Fournier, Florence Fenollar
Summary: Bacterial strain Marseille-P3954 was isolated from a stool sample of a 35-year-old male patient living in France. It belongs to a new bacterial genus, classified in a new family, based on its genomic, phenotypic, and phylogenetic evidences. The proposed classification is Maliibacterium massiliense gen. nov., sp. nov. with the type strain Marseille-P3954 (CSUR P3954 = CECT 9568).
CURRENT MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Nasserdine Papa Mze, Idir Kacel, Mamadou Beye, Raphael Tola, Mariema Sarr, Leonardo Basco, Herve Bogreau, Philippe Colson, Pierre-Edouard Fournier
Summary: A rapid and cost-effective sequencing protocol is proposed to validate its reliability by comparing the SARS-CoV-2 genome sequences obtained using the modified Illumina COVIDSeq protocol with the standard protocol. The modified protocol saves time and costs, providing high-quality results comparable to the standard protocol.
Article
Microbiology
Younes Laidoudi, Elodie Rousset, Anne-Sophie Dessimoulie, Myriam Prigent, Alizee Raptopoulo, Quentin Huteau, Elisabeth Chabbert, Catherine Navarro, Pierre-Edouard Fournier, Bernard Davoust
Summary: Thirteen cases of Q fever in a area in southern France were likely linked to a sheepfold, which had widespread bacterial contamination. This highlights the need for stronger and improved surveillance of Q fever.
Article
Infectious Diseases
Juan Ambrosioni, Marta Hernandez-Meneses, Emanuele Durante-Mangoni, Pierre Tattevin, Lars Olaison, Tomas Freiberger, John Hurley, Margaret M. Hannan, Vivian Chu, Bruno Hoen, Asuncion Moreno, Guillermo Cuervo, Jaume Llopis, Jose M. Miro
Summary: This study compared the epidemiological features and outcomes of infective endocarditis (IE) in different European regions and time periods in the 21st century. The results showed that despite an increase in the complexity of IE cases, prognosis has improved in recent years with a significant decrease in 6-month mortality. The source of infection differed between regions, but the outcomes were not different.
INFECTIOUS DISEASES AND THERAPY
(2023)
Expression of Concern
Immunology
S. Meconi, C. Capo, M. Remacle-Bonnet, G. Pommier, D. Raoult, J. L. Mege
INFECTION AND IMMUNITY
(2023)
Correction
Infectious Diseases
Thi Loi Dao, Naomie Canard, Van Thuan Hoang, Tran Duc Anh Ly, Tassadit Drali, Laetitia Ninove, Florence Fenollar, Didier Raoult, Philippe Parola, Pierre Marty, Philippe Gautret
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Expression of Concern
Microbiology
T. Adekambi, M. Reynaud-Gaubert, G. Greub, M-J Gevaudan, B. La Scola, D. Raoult, D. Drancourt
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Expression of Concern
Microbiology
W. Xu, D. Raoult
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Immunology
Babacar Mbaye, Reham Magdy Wasfy, Maryam Tidjani Alou, Patrick Borentain, Claudia Andrieu, Aurelia Caputo, Didier Raoult, Rene Gerolami, Matthieu Million
Summary: This study analyzed the microbial composition of NASH patients and controls using 16S rRNA sequencing and qPCR. The results showed an association between Lactobacillus and Lactoccocus with NASH, and an association between Methanobrevibacter, Faecalibacterium, and Romboutsia with controls. At the species level, ethanol-producing species of Lactobacillus and an already associated species of dysbiosis were associated with NASH. This study highlights the potential role of ethanol-producing gut microbes in NASH.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Farah Azouzi, Louis Olagne, Sophie Edouard, Serge Cammilleri, Pierre-Edouard Magnan, Pierre-Edouard Fournier, Matthieu Million
Summary: Cardiovascular infections caused by Coxiella burnetii are the most severe and potentially lethal among persistent localized infections. The use of 18-FDG PET scans has allowed for the detection of non-aortic vascular infections, expanding clinicians' understanding of these infections beyond the heart and aorta.
Article
Microbiology
Fatou Samba Diouf, Mamadou Beye, Mapenda Gaye, Babacar Mbaye, Stephane Alibar, Mariema Sarr, Gregory Dubourg, Jean-Christophe Lagier, Cheikh Sokhna, Florence Fenollar, Pierre-Edouard Fournier, Cheikh Ibrahima Lo
Summary: Blood is normally sterile, but bacteria in the bloodstream indicate an anomaly. By performing a blood culture on a patient with thalassemia major, a new strain of Streptococcus was isolated and identified through phenotypic, phylogenetic, and genomic characteristics analysis. Named Streptococcus thalassemiae sp. nov., this strain showed similarity to Streptococcus mitis but had significant differences based on DNA-DNA hybridization and average nucleotide identity analysis. This research demonstrates the importance of studying bacterial strains in blood cultures for accurate diagnosis.
INTERNATIONAL JOURNAL OF MICROBIOLOGY
(2023)
Correction
Microbiology
Cheikh Ibrahima Lo, Niokhor Dione, Aminata Mbaye, Patricia Fernandez-Mellado Gomez, Issa Isaac Ngom, Camille Valles, Stephane Alibar, Jean-Christophe Lagier, Florence Fenollar, Pierre-Edouard Fournier, Didier Raoult, Seydina M. Diene
FEMS MICROBIOLOGY LETTERS
(2023)
