Article
Chemistry, Multidisciplinary
Marjon Zamani, James M. Robson, Andy Fan, Michael S. Bono, Ariel L. Furst, Catherine M. Klapperich
Summary: Sexually transmitted infections, such as HIV and HPV, have a disproportionate impact on low-resource settings. Early diagnosis is crucial for managing HIV and preventing HPV-induced cervical cancer. A novel electrochemical platform combined with loop-mediated isothermal amplification and CRISPR-based detection shows promise for rapidly and cost-effectively detecting viral infections.
ACS CENTRAL SCIENCE
(2021)
Article
Microbiology
Yongjin Qiu, Martin Simuunza, Masahiro Kajihara, Joseph Ndebe, Ngonda Saasa, Penjani Kapila, Hayato Furumoto, Alice C. C. Lau, Ryo Nakao, Ayato Takada, Hirofumi Sawa
Summary: Tick-borne diseases are of global public health importance but often overlooked in countries with fewer resources. This study investigated the presence of pathogens in ticks collected from dogs and cattle in a border town between Zambia and Angola, and identified several pathogenic species. The findings highlight the need for awareness of these pathogens in the area.
Review
Fisheries
Melbourne Rio Talactac, Emmanuel Pacia Hernandez, Takeshi Hatta, Kentaro Yoshii, Kodai Kusakisako, Naotoshi Tsuji, Tetsuya Tanaka
Summary: Ticks, as obligate hematophagous arthropods, are exposed to various blood-borne pathogens, including arboviruses, and their feeding behavior can transmit economically important viral pathogens to humans and animals. The replication and transmission of tick-borne viruses must be carefully regulated by their tick vectors to avoid negative effects on tick development. However, research on the tick-virus interface lags behind that of mosquitoes, due to the complexity of tick feeding behavior, limiting the understanding of tick immunity against tick-borne viruses.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Microbiology
Li-Zhu Fang, Si-Cong Lei, Zhi-Jian Yan, Xiao Xiao, Jian-Wei Liu, Xiao-Qing Gong, Hao Yu, Xue-Jie Yu
Summary: Tickborne intracellular bacterial pathogens such as Anaplasma, Coxiella burnetti, Ehrlichia, and Rickettsia were detected in Haemaphysalis flava ticks from hedgehogs in Central China. The prevalence of these pathogens varied among different developmental stages of ticks, with the highest infection rate observed in dead engorged females.
Article
Chemistry, Analytical
Rui Zhang, Pu Wang, Yangyang Chang, Meng Liu
Summary: Rapid and sensitive detection of ATP in bioaerosols is crucial. The strategy of interfacing split DNA aptamers with MXene-based composite materials provides a versatile approach for the ubiquitous detection of biochemical targets in bioaerosols.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Hye Kyu Choi, Jinho Yoon
Summary: The accurate and rapid diagnosis of viral diseases using biosensors has gained attention in recent years. Nanotechnology offers a promising strategy to overcome the limitations of conventional viral nucleic acid-based biosensors. By implementing nanotechnologies, such as functional nanomaterials and surface nanoengineering, the performance and range of sensing targets of biosensors can be improved. This review provides interdisciplinary information on nanotechnology-assisted biosensors, giving a valuable basis for the development of novel viral nucleic acid biosensors.
Review
Biochemistry & Molecular Biology
Sandile Phinda Songca
Summary: Nanozymes are synthetic nanoparticulate materials that mimic biological activities of enzymes, used for detection and identification of pathogens efficiently, offering cost-effectiveness and stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Yang Bu, Sheng Ni, Levent Yobas
Summary: This article investigates the mechanism of synchronous coefficient of drag alteration and finds that using a microfabricated artificial sieve can reduce the purification time of DNA from hours to minutes. An electrophoretic protocol is also proposed to facilitate the collection of purified DNA from the sieve, which can be used for rapid diagnostic applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Parasitology
Ahmet Efe Koseoglu, Huseyin Can, Mervenur Guvendi, Sedef Erkunt Alak, Cagri Kandemir, Turgay Taskin, Samiye Demir, Gulsah Akgul, Aysu Degirmenci Doskaya, Muhammet Karakavuk, Mert Doskaya, Adnan Yuksel Guruz, Cemal Un
Summary: The study aimed to investigate tick-borne pathogens in 110 tick samples collected from different hosts in Turkey, and found Hepatozoon spp., Theileria spp., Babesia spp., and Anaplasma spp. at various rates. Theileria ovis and Anaplasma ovis were detected for the first time in certain tick species in Turkey, along with the first detection of Babesia caballi in ticks in the country.
PARASITES & VECTORS
(2021)
Review
Cell Biology
Xin Yong, Lejiao Mao, Xiaofei Shen, Zhen Zhang, Daniel D. Billadeau, Da Jia
Summary: Endosomes are crucial cellular stations for protein trafficking, where proteins can be degraded or recycled to different cellular destinations. Recent studies have shown that pathogens like bacteria and viruses exploit host endosomal recycling pathways for their survival and replication. This manipulation of host signaling pathways by pathogens deepens our understanding of the molecular intricacies regulating endosomal trafficking.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Rochelle Payne Ondracek, Jianhong Chen, Beth Marosy, Sirinapa Szewczyk, Leonard Medico, Amrutha Sherly Mohan, Priya Nair, Rachel Pratt, Janise M. Roh, Thaer Khoury, John Carpten, Lawrence H. Kushi, Julie R. Palmer, Kim Doheny, Warren Davis, Michael J. Higgins, Song Yao, Christine B. Ambrosone
Summary: This study reports the results and lessons learned from processing FFPE breast tumor tissues for a large epidemiologic study. The dual DNA/RNA purification using Qiagen AllPrep FFPE extraction protocol is feasible for clinical and epidemiologic studies. The use of tissue punches as a reliable source material and the fine tuning of proteinase K digestion time based on tissue volume are critical for achieving balanced yields of DNA and RNA.
Review
Veterinary Sciences
Lucas Lannutti, Fernanda Noemi Gonzales, Maria Jose Dus Santos, Monica Florin-Christensen, Leonhard Schnittger
Summary: The honeybee Apis mellifera is highly valued for its pollination services and products, but it is also susceptible to infections caused by various pathogens. Infections can easily spread within and between beehives due to the close contact and foraging habits of bees. International trade of bees has contributed to the global spread of infections, leading to significant losses in apiculture. Molecular methods have significantly advanced the study of bee pathogens, allowing for precise identification, analysis of co-infections, description of new species, associations between genotypes and phenotypes, and population structure studies.
VETERINARY SCIENCES
(2022)
Article
Microbiology
Alexandr Morozov, Alexei Tischenkov, Cornelia Silaghi, Andrei Proka, Ion Toderas, Alexandru Movila, Hagen Frickmann, Sven Poppert
Summary: The study investigated the presence of ticks on birds in Moldova and detected various tick species, as well as DNA of pathogens in the ticks, providing new insights into the epidemiology of tick-transmitted infectious diseases in the region.
Article
Microbiology
Yannick Ngnindji-Youdje, Adama Zan Diarra, Michel Lontsi-Demano, Timoleon Tchuinkam, Philippe Parola
Summary: Ticks in Cameroon carry a variety of microorganisms and pose a risk of zoonotic diseases.
Review
Oncology
Manikandan Palrasu, Elena Zaika, Wael El-Rifai, Jianwen Que, Alexander I. Zaika
Summary: Stomach cancer is a common and deadly malignancy worldwide, with infections by Helicobacter pylori and Epstein-Barr virus being major risk factors. The complex interplay between microbial, environmental, and host genetic factors underlies gastric tumorigenesis, with many aspects still unclear. Recent studies focus on the roles of various gastric pathogens and the gastric microbiome in tumorigenesis.
