Article
Biotechnology & Applied Microbiology
Yuqing Niu, Massimiliano Galluzzi, Fuming Deng, Zhang Zhao, Ming Fu, Liang Su, Weitang Sun, Wei Jia, Huimin Xia
Summary: The study found that the hyaluronic acid-coating silk fibroin nanofiber scaffold can effectively regulate the epithelialization process of urethral regeneration, promoting luminal epithelialization, smooth muscle bundle structural remodeling, and capillary formation.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Chemistry, Applied
Weiguang Zhao, Shiyi Cao, Haoxin Cai, Yang Wu, Qi Pan, Heng Lin, Jun Fang, Yinyan He, Hongbing Deng, Zhihong Liu
Summary: This study addresses the challenge of reconstructing multilayered and functional smooth muscle tissues by incorporating cellulose acetate electrospun nanofibers into chitosan/silk fibroin cryogel scaffolds. The composite scaffolds showed improved mechanical properties and enhanced proliferation and infiltration of smooth muscle cells. The findings indicate the potential of these scaffolds for smooth muscle tissue engineering.
CARBOHYDRATE POLYMERS
(2022)
Review
Medicine, Research & Experimental
Augusto Zuluaga-Velez, Adrian Quintero-Martinez, Lina M. Orozco, Juan C. Sepulveda-Arias
Summary: Silk fibroin nanocomposites have great potential in tissue engineering, with the ability to enhance stability and mechanical performance by incorporating inorganic phases and influencing specific biological responses. However, challenges still exist in their application to other tissues, calling for further research on the interaction between inorganic and organic phases and the associated biological responses.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Pharmacology & Pharmacy
Zohre Montaseri, Samira Sadat Abolmaali, Ali Mohammad Tamaddon, Fakhrossadat Farvadi
Summary: Cartilage tissue engineering requires an appropriate microenvironment for chondrocyte proliferation. Composite silk fibroin hydrogels have shown strong potential and advantages for cartilage regeneration.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Xiuli Wang, Chengchen Guo, Lina Guo, Mingqi Wang, Ming Liu, Yizhe Song, Hui Jiao, Xiaoqing Wei, Zinan Zhao, David L. Kaplan
Summary: A versatile strategy was developed to fabricate biomimetic hepatic lobules in vitro, utilizing silk protein scaffolds with radially aligned lamellar sheets and interconnected channels. Coculturing human hepatocytes and primary endothelial cells on these scaffolds resulted in enhanced hepatocyte functions and physiological cell organization, replicating hepatic-like parenchymal-mesenchymal growth patterns. This biomimetic technology holds potential for applications in bioartificial liver engineering and drug metabolism investigation.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Physical
Sylwia Grabska-Zielinska, Adrianna Sosik, Anna Malkowska, Ewa Olewnik-Kruszkowska, Kerstin Steinbrink, Konrad Kleszczynski, Beata Kaczmarek-Szczepanska
Summary: In this study, two-component dialdehyde chitosan/hyaluronic acid scaffolds were developed and characterized, showing a porous structure with interconnected pores. The scaffold composition had an influence on physicochemical properties, but there were no significant differences in cell viability proliferation among all scaffolds.
Article
Engineering, Biomedical
Xiaohang Qu, Ling Yan, Shuang Liu, Yunfei Tan, Jing Xiao, Yuan Cao, Ke Chen, Wenqian Xiao, Bo Li, Xiaoling Liao
Summary: Silk fibroin (SF) from Bombyx mori is a natural polymer with excellent biocompatibility, but traditional SF hydrogels may have some drawbacks. By inducing the formation of uniform beta-sheet structures through sonication and enzymatic crosslinking, a composite hydrogel with enhanced strength and elasticity was fabricated, showing concentration-dependent stiffness and time-dependent toughness variations. This biocompatible composite hydrogel with unique mechanical properties holds great potential for tissue engineering and regenerative medicine.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2021)
Article
Biochemistry & Molecular Biology
Monika Rajput, Pritiranjan Mondal, Parul Yadav, Kaushik Chatterjee
Summary: Three-dimensional bioprinting based on digital light processing (DLP) provides opportunities for mimicking human tissues. However, the limited availability of biocompatible and biodegradable bioinks hinders progress in this field. This study presents a silk fibroin-derived bioink for DLP bioprinting, which exhibits bone-like viscoelastic behavior and favorable degradation. The bioink supports cell proliferation and osteogenesis. This work highlights the importance of silk fibroin-derived bioinks in tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Polymer Science
Ponrasu Thangavel, Hemalatha Kanniyappan, Sudip Chakraborty, Shipra Chaudhary, Aadinath Wallepure, Vignesh Muthuvijayan
Summary: Biomimetic scaffolds were prepared using konjac glucomannan (KGM) and silk fibroin (SF), which were cross-linked with citric acid and lyophilized to obtain fibrous structures. The KGM/SF scaffolds exhibited improved thermal stability, porous morphology, and biodegradable nature. They also showed enhanced compression strength and demonstrated biocompatibility, cell viability, and vascularization efficacy in tissue engineering and biomedical applications.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Lina Guo, Ziqing Zhu, Chuanzhou Gao, Kaiwen Chen, Shenzhou Lu, Hexin Yan, Wenming Liu, Mingqi Wang, Yanfang Ding, Lin Huang, Xiuli Wang
Summary: By developing SF-collagen I composite scaffolds with improved physical and chemical properties, a more biomimetic hepatic lobule-like model was successfully bioengineered, allowing iHepLPCs to differentiate into mature hepatocytes with enhanced liver functions and facilitating the arrangement of hepatocyte-endothelial cell interphase with improved polarity and bile capillary formation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Xin Liu, Yuxiang Sun, Bo Chen, Yan Li, Peng Zhu, Peng Wang, Sen Yan, Yao Li, Fang Yang, Ning Gu
Summary: A novel magnetic SF scaffold (MSFCs) was prepared by an improved infiltration method, exhibiting better crystallinity, magnetocaloric properties, and mechanical strength compared to SF scaffold (SFC). The delayed degradation of MSFCs was attributed to the formation of hydrogen bonds and complexing to tyrosine to inhibit hydrolase by internal iron atoms. Doping MNPs showed a beneficial effect on beta-crystallization of protein and did not affect the function of macrophages.
BIOACTIVE MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Weizhen Sun, David Alexander Gregory, Mhd Anas Tomeh, Xiubo Zhao
Summary: Tissue engineering involves combining cells, scaffold materials, and growth factors to regenerate or replace damaged tissues or organs. Silk fibroin has attracted attention for its outstanding properties and ability to be reconstructed into various material formats for tissue engineering applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Guangfei Li, Shan Sun
Summary: Silk fibroin scaffolds are widely used materials in tissue engineering, with excellent properties and diverse forms that can be achieved through various fabrication techniques. These scaffolds play a significant role in various tissue engineering applications.
Article
Materials Science, Textiles
Zhenyu Xu, Ke Li, Kui Zhou, Shuiyuan Li, Hongwei Chen, Jiaqi Zeng, Rugang Hu
Summary: This study investigates the effects of different concentrations of silk fibroin solutions on the physicochemical and biological properties of silk-hydroxyapatite-sodium alginate scaffolds. The results show that higher concentrations of silk fibroin can promote cell proliferation and meet the requirements for bone tissue repair, providing important reference for bone tissue engineering applications.
FIBERS AND POLYMERS
(2023)
Article
Biochemistry & Molecular Biology
Natasha Bokhari, Asif Ali, Abida Yasmeen, Hamad Khalid, Sher Zaman Safi, Faiza Sharif
Summary: Soft tissue defects can be resolved with coated silk meshes, which have antimicrobial and wound healing properties. The coating alters the pore size and fiber diameter of the mesh, while also improving its mechanical strength. Among the variants tested, MHRJ showed the best cell viability and wound healing effects. In vivo experiments with rat hernia model confirmed the non-toxic nature and wound healing characteristics of the modified mesh. This study suggests that MHRJ could be a valuable biomedical implant.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)